A5051498754- Nanoparticles: synthesis and applications
- RNA Interference and Gene Delivery
- Nanoparticle-Based Drug Delivery
- Advanced biosensing and bioanalysis techniques
- Antioxidant Activity and Oxidative Stress
- Molecular Junctions and Nanostructures
- Gold and Silver Nanoparticles Synthesis and Applications
- Neuroscience and Neural Engineering
- Monoclonal and Polyclonal Antibodies Research
- Phytochemicals and Antioxidant Activities
- Biosensors and Analytical Detection
- Graphene and Nanomaterials Applications
- 3D Printing in Biomedical Research
- Free Radicals and Antioxidants
- Neuroendocrine Tumor Research Advances
- Neuroblastoma Research and Treatments
- Phytoplasmas and Hemiptera pathogens
- Atherosclerosis and Cardiovascular Diseases
- Edible Oils Quality and Analysis
- Viral Infectious Diseases and Gene Expression in Insects
- Nanomaterials and Printing Technologies
- Nanofabrication and Lithography Techniques
- Lipid Membrane Structure and Behavior
- Field-Flow Fractionation Techniques
- Biotin and Related Studies
Istituto di Nanotecnologia
2020-2024
National Research Council
2018-2023
Sapienza University of Rome
2022
University of Salento
2022
Division of Undergraduate Education
2022
Cloud Computing Center
2022
Institute of Sciences of Food Production
2017-2021
Italian Institute of Technology
2010-2017
Center for Biomolecular Nanotechnologies
2010-2016
Istituto Nanoscienze
2009
The development of appropriate in vitro protocols to assess the potential toxicity ever expanding range nanoparticles represents a challenging issue, because rapid changes their intrinsic physicochemical properties (size, shape, reactivity, surface area, etc.) upon dispersion biological fluids. Dynamic formation protein coating around is key molecular event, which may strongly impact response nanotoxicological tests. In this work, by using citrate-capped gold (AuNPs) different sizes as...
The interaction between cells and nanostructured materials is attracting increasing interest, because of the possibility to open up novel concepts for design smart nanobiomaterials with active biological functionalities. In this frame we investigated response human neuroblastoma cell line (SH-SY5Y) gold surfaces different levels nanoroughness. To achieve a precise control nanoroughness nanometer resolution, exploited wet chemistry approach based on spontaneous galvanic displacement reaction....
Point mutations in the Kirsten rat sarcoma viral oncogene homologue (KRAS) gene are being increasingly recognized as important diagnostic and prognostic markers cancer. In this work, we describe a rapid low-cost method for naked-eye detection of cancer-related point KRAS based on gold nanoparticles. This simple colorimetric assay is sensitive (limit low picomolar range), instrument-free, employs nonstringent room temperature conditions due to combination DNA-conjugated nanoparticles, probe...
Anthocyanins, the naturally occurring pigments responsible for most red to blue colours of flowers, fruits and vegetables, have also attracted interest because their potential health effects. With aim contributing major insights into structure-activity relationship (SAR), we evaluated radical scavenging biological activities selected purified anthocyanin samples (PASs) from various anthocyanin-rich plant materials: two (mahaleb cherry blackcurrant) vegetables (black carrot "Sun Black"...
A novel seed-mediated synthetic route to produce multibranched gold nanoparticles is reported, in which it possible precisely tune both their size and nanostructuration, while maintaining an accurate level of monodispersion. The nanoscale control surface nanoroughness/branching, ranging from small bud-like features elongated spikes, allows obtain fine tuning the nanoparticle optical properties, up red near-IR region spectrum. Such anisotropic nanostructures were demonstrated be excellent...
The expected potential benefits promised by nanotechnology in various fields have led to a rapid increase of the presence engineered nanomaterials high number commercial goods. This is generating increasing questions about possible risks for human health and environment, due lack an in-depth assessment physical/chemical factors responsible their toxic effects. In this work, we evaluated toxicity monodisperse citrate-capped gold nanoparticles (AuNPs) different sizes (5, 15, 40, 80 nm) model...
Tomato (Solanum lycopersicum L.) is one of the most cultivated vegetable in world and it represents a large source bioactive compounds, including carotenoids polyphenols (phenolic acids flavonoids). However, concentration flavonoids tomato considered sub-optimal, particularly because anthocyanins are not generally present. Therefore, this crop has been object an intense metabolic engineering order to obtain anthocyanin-enriched tomatoes by using either breeding or transgenic strategies. Some...
The carrot is one of the most cultivated vegetables in world. Black or purple carrots contain acylated anthocyanins which are special interest to food industry for their stability and nutraceutical characteristics. Anthocyanin-rich fruits have gained popularity last ten years, due health benefits they provide. In this paper, characterizations bioactive compounds antioxidant capacities different anthocyanin-containing (a black carrot—BC, a local carrot, “Polignano” carrot—PC), compared...
In this work, we propose a systematic and reproducible evaluation of nanoparticles (NPs) toxicology in living systems, based on physical assessment quantification the toxic effects NPs by experimental determination key parameter affecting toxicity outcome (i.e., number NPs) "toxicity factor". Such strategy was applied to well determined scenario, i.e., ingestion citrate-capped gold (AuNPs) different sizes model system Drosophila melanogaster. Using these AuNPs as reference standard, were...
The existing manufacturing protocols for CAR-T cell therapies pose notable challenges, particularly in attaining a transient transfection that endures significant duration. To address this gap, study aims to formulate protocol utilizing multiple lipid-based nanoparticles (LNPs) administrations enhance efficiency (TE) clinically relevant levels. By systematically fine-tuning and optimizing our through series of iterative refinements, we have accomplished remarkable one-order-of-magnitude...
Nano-delivery systems represent one of the most studied fields, thanks to associated improvement in treatment human diseases. The functionality nanostructures is a crucial point, which effectiveness nanodrugs depends on. A hybrid approach strategy using synthetic nanoparticles (NPs) and erythrocytes offers an optimal blend natural materials. This, turn, allows medical practitioners exploit combined advantages NPs. Erythrocyte-based drug delivery have been investigated for their...
Olive quick decline syndrome (OQDS) causes severe damages to the olive trees in Salento (Apulia, Italy) and poses a threat for agriculture of Mediterranean countries. DNA-based typing methods have pointed out that OQDS is caused by single outbreak strain Xylella fastidiosa subsp. pauca referred as CoDiRO or ST53. Since no effective control measures are currently available, objective this study was evaluate vitro antimicrobial activities different classes compounds against Salento-1 isolated...
For centuries, macroalgae, or seaweeds, have been a significant part of East Asian diets. In Europe, seaweeds are not considered traditional foods, even though they increasingly popular in Western diets human food applications. this study, biological processing method based on semi-solid fermentation was optimized for the treatment seaweed Gracilaria gracilis. first time, selected lactic acid bacteria and non-conventional coagulase-negative staphylococci were used as starter preparations...
We show the development of a new class highly efficient, biocompatible fiducial markers for X-ray imaging and radiosurgery, based on polymer shells encapsulating engineered gold nanoparticle (AuNP) suspensions. Our smart fabrication strategy enables wide tunability size, shape, attenuation performance, up to record values >20 000 Hounsfield units (HU), i.e. comparable or even higher than bulk gold. that NP fiducials allow superior both in vitro vivo (yet requiring 2 orders magnitude less...
Inkjet printing is considered a key technology in the fabrication of organic light-emitting diodes (OLEDs), but achieving fully inkjet-printable OLED structure still challenge. Here, we propose OLEDs with an inkjet-printed multilayer (i.e. anode/hole injection layer (HIL)/electron blocking (EBL)/emitting (EML)) by properly developing new HIL and EBL inks to achieve uniform homogeneous printed thin films. In fabricated device, dissolution process EBL, which occurs during EML, creates blurred...