A5045420537- Phytochemicals and Antioxidant Activities
- Mitochondrial Function and Pathology
- Antioxidant Activity and Oxidative Stress
- Photosynthetic Processes and Mechanisms
- Adipose Tissue and Metabolism
- Plant Stress Responses and Tolerance
- Heavy Metals in Plants
- Free Radicals and Antioxidants
- Crop Yield and Soil Fertility
- Genomics, phytochemicals, and oxidative stress
- Pomegranate: compositions and health benefits
- Biochemical and biochemical processes
- Lipid metabolism and biosynthesis
- GABA and Rice Research
- Cardiac Ischemia and Reperfusion
- ATP Synthase and ATPases Research
- Consumer Attitudes and Food Labeling
- Advanced Glycation End Products research
- Seed and Plant Biochemistry
- Plant Micronutrient Interactions and Effects
- Food composition and properties
- Bee Products Chemical Analysis
- Wheat and Barley Genetics and Pathology
- Cassava research and cyanide
- Plant responses to water stress
University of Foggia
2015-2025
Ambiente Italia (Italy)
2016
Background and objectives Mutations in the TRPC6 gene have been recently identified as cause of late-onset autosomal-dominant focal segmental glomerulosclerosis (FSGS). To extend screening, we analyzed 33 Italian children with sporadic early-onset SRNS three families adult-onset FSGS. Design, setting, participants, & measurements mutation analysis was performed through PCR sequencing. The effects detected amino acid substitutions were by bioinformatics tools functional vitro studies....
The carrot is one of the most cultivated vegetables in world. Black or purple carrots contain acylated anthocyanins which are special interest to food industry for their stability and nutraceutical characteristics. Anthocyanin-rich fruits have gained popularity last ten years, due health benefits they provide. In this paper, characterizations bioactive compounds antioxidant capacities different anthocyanin-containing (a black carrot—BC, a local carrot, “Polignano” carrot—PC), compared...
Abstract: Antioxidant activity (AA) of quinoa ( Chenopodium Willd.) seeds, as well durum wheat Triticum turgidum L. ssp. Desf.) and emmer T . dicoccum Schübler) grains, was evaluated by studying hydrophilic (H), lipophilic (L), free‐soluble (FSP) insoluble‐bound (IBP) phenolic extracts using the new lipoxygenase/4‐nitroso‐ N,N‐ dimethylaniline (LOX/RNO) method, able to simultaneously detect different antioxidant mechanisms, Oxygen Radical Absorbance Capacity (ORAC) Trolox Equivalent (TEAC)...
The effect of free fatty acids (FFAs) and acyl-CoA esters on K+ uptake was studied in mitochondria isolated from durum wheat (Triticum Desf.), a species that has adapted well to the semi-arid Mediterranean area possessing highly active mitochondrial ATP-sensitive channel (PmitoKATP), may confer resistance environmental stresses. This made by swelling experiments KCl solution under experimental conditions which PmitoKATP activity monitored. Linoleate other FFAs (laurate, palmitate, stearate,...
Abstract Current in vitro methodologies neglect or subestimate the contribution of betalains to antioxidant capacity foods because they do not reflect their vivo biological mechanisms. In this study, we assessed sensibility lipoxygenase-fluorescein (LOX-FL) method towards betalains, phenolic compounds and ascorbic acid from Opuntia spp. fruits; (ii) peel pulp extracts ficus-indica L. Mill (var. Fresa, Colorada Blanco) stricta var. Dillenii ; by comparing LOX-FL traditional methods (ORAC...
To date, the existence of plant inner membrane anion channel (PIMAC) has been shown only in potato mitochondria, but its physiological role remains unclear. In this study, by means swelling experiments K+ and ammonium salts, we characterize a PIMAC-like anion-conducting pathway mitochondria from durum wheat (DWM), monocotyledonous species phylogenetically far potato. DWM were investigated since they possess very active potassium (PmitoKATP), so implying matching uniport and, possibly,...
The important role of plant mitochondria in the adaptation to environmental stresses at subcellular level has recently emerged. In particular, an mitochondrial mechanism involved resistance is inhibition proline oxidation. order study this physiological mechanism, we used both washed and purified durum wheat ( Triticum ) (DWM) isolated from early seedlings germinated two different NaCl solutions leading either moderate or severe damage growth. To assess contribution osmotic component stress,...
The health-promoting properties of natural plant bioactive compounds are mainly attributable to their ability counteract oxidative stress. This is considered a major causative factor in aging and aging-related human diseases, which causal role also ascribed dicarbonyl due accumulation methylglyoxal (MG) other reactive species, leading macromolecule glycation cell/tissue dysfunction. glyoxalase (GLYI) enzyme, catalyzing the rate-limiting step GSH-dependent MG detoxification pathway, plays key...
Indirect evidence points to the presence of K+ channels in plant mitochondria. In present study, we report results first patch clamp experiments on Single-channel recordings 150 mM potassium gluconate have allowed biophysical characterization a channel with conductance pS inner mitochondrial membrane mitoplasts obtained from wheat (Triticum durum Desf.). The displayed sharp voltage sensitivity, permeability and cation selectivity. ATP concentration range completely abolished activity. We...
Mitigation practices for cereal systems, including conservation agriculture and low emission fertilization, are required to face global challenges of food security climate change. The combination these climate-smart approaches was investigated durum wheat in a dry region the Mediterranean basin two crop seasons. experimental design consisted different genotypes, Marco Aurelio (high protein content) Saragolla (higher adaptability), subjected no tillage (NT) vs. conventional (CT) fertilization...
In this study the 4-nitroso-N,N-dimethylaniline (RNO) bleaching associated with linoleic acid hydroperoxidation catalyzed by soybean lipoxygenase (LOX)-1 isoenzyme (LOX/RNO reaction) was used to determine antioxidant activity (AA) of hydrophilic and lipophilic pure compounds mixtures antioxidants extracted from durum wheat whole flour (DWWF). By means a simple rapid experimental protocol (about 3 min/assay), LOX/RNO reaction may simultaneously detect many functions (scavenging some...
Durum wheat mitochondria (DWM) possess an ATP-inhibited K(+) channel, the plant mitoK(ATP) (PmitoK(ATP) ), which is activated under environmental stress to control mitochondrial ROS production. To do this, PmitoK(ATP) collapses membrane potential (ΔΨ), thus suggesting uncoupling. We tested this point by studying oxidative phosphorylation (OXPHOS) in DWM purified from seedlings and subjected both severe mannitol NaCl stress. In severely-stressed DWM, ATP synthesis via OXPHOS, continuously...
The new LOX-FL method highlights improvement of serum antioxidant capacity after intake better than widely used methods.