A5019338245- Nanoparticles: synthesis and applications
- Immunotherapy and Immune Responses
- Contact Dermatitis and Allergies
- Air Quality and Health Impacts
- Protease and Inhibitor Mechanisms
- Occupational exposure and asthma
- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
- Indoor Air Quality and Microbial Exposure
- Inhalation and Respiratory Drug Delivery
- MicroRNA in disease regulation
- Cell Adhesion Molecules Research
- Nanoparticle-Based Drug Delivery
- Animal testing and alternatives
- Electrochemical Analysis and Applications
- Glycosylation and Glycoproteins Research
- Gold and Silver Nanoparticles Synthesis and Applications
- Heavy Metal Exposure and Toxicity
- Systemic Lupus Erythematosus Research
- Immune Response and Inflammation
- Digital Imaging for Blood Diseases
- Advanced biosensing and bioanalysis techniques
- Asthma and respiratory diseases
- Dermatology and Skin Diseases
- Systemic Sclerosis and Related Diseases
Flemish Institute for Technological Research
2016-2025
Hasselt University
2022
Friedrich-Alexander-Universität Erlangen-Nürnberg
2021
Freie Universität Berlin
2021
Luxembourg Institute of Science and Technology
2021
Fraunhofer Institute for Ceramic Technologies and Systems
2021
Province of Antwerp
2017
University of Milan
2011
Rega Institute for Medical Research
1999-2006
KU Leuven
1999-2003
Matrix metalloproteinases (MMPs) form a family of enzymes with major actions in the remodeling extracellular matrix (ECM) components. Gelatinase B (MMP-9) is most complex member terms domain structure and regulation its activity. activity under strict control at various levels: transcription gene by cytokines cellular interactions; activation pro-enzyme cascade comprising serine proteases other MMPs; specific tissue inhibitors MMPs (TIMPs) or unspecific inhibitors, such as...
Summary The phytoalexin‐deficient Arabidopsis mutant pad3‐1 , which is affected in the production of indole‐type phytoalexin camalexin, has previously been shown not to display altered susceptibility either bacterium Pseudomonas syringae ( Glazebrook & Ausubel 1994 ; Proc. Natl. Acad. Sci. USA 91: 8955–8959) or biotrophic fungi Peronospora parasitica et al . 1997 Genetics 146: 381–392) and Erysiphe orontii Reuber 1998 Plant J. 16: 473–485). We now show that this markedly more susceptible...
Nanosized objects, such as nanoparticles and other drug carriers used in nanomedicine, once contact with biological environments are modified by adsorption of biomolecules on their surface. The presence this corona strongly affects the following interactions at cell organism levels. It has been shown that proteins can be recognized receptors. However, it is not known whether composition acquired layer also affect mechanisms use to enter cells. This particular importance when considering same...
With the increasing use of nanomaterials, need for methods and assays to examine their immunosafety is becoming urgent, in particular nanomaterials that are deliberately administered human subjects (as case nanomedicines). To obtain reliable results, standardised vitro immunotoxicological tests should be used determine effects engineered nanoparticles on immune responses. However, before can standardised, it important suitable established validated. In a collaborative work between European...
Blood vessel formation or angiogenesis is a key process for successful tooth regeneration. Bone marrow-derived mesenchymal stromal cells (BM-MSCs) possess paracrine proangiogenic properties, which are, at least partially, induced by their extracellular vesicles (EVs). However, the isolation of BM-MSCs associated with several drawbacks, could be overcome MSC-like teeth, called dental pulp (DPSCs). This study aims to compare angiogenic content and functions DPSC BM-MSC EVs conditioned medium...
Abstract Cell culture‐conditioned medium (CCM) is a valuable source of extracellular vesicles (EVs) for basic scientific, therapeutic and diagnostic applications. culturing parameters affect the biochemical composition, release possibly function CCM‐derived EVs (CCM‐EV). The CCM‐EV task force Rigor Standardization Subcommittee International Society Extracellular Vesicles aims to identify relevant cell parameters, describe their effects based on current knowledge, recommend reporting...
Suitable assays and test strategies are needed to analyze potential genotoxic immunotoxic health effects caused by nanoparticle exposure. The development validation of such methods is challenging because nanoparticles may show unexpected behavior, like aggregation or interference with optical measurements, when routine in vitro performed. In our interdisciplinary study, the inorganic gold (4.5 nm) iron oxide (7.3 a narrow size distribution were tested on human cells using different assay...
Background Dendritic cells (DCs) comprise heterogeneous populations of cells, which act as central orchestrators the immune response. Applicability primary DCs is restricted due to their scarcity and therefore DC models are commonly employed in DC-based immunotherapy strategies vitro tests assessing function. However, interrelationship between individual relative resemblance specific remain elusive. Objective To describe assess functionality applicability available by using a genome-wide...
A central challenge for the safe design of nanomaterials (NMs) is inherent variability NM properties, both as produced and they interact with evolve in, their surroundings. This has led to uncertainty in literature regarding whether biological toxicological effects reported NMs are related specific properties themselves, or rather presence impurities physical such agglomeration particles. Thus, there a strong need systematic evaluation synthesis processing parameters that lead potential...
Titanium dioxide (TiO2) is widely used in pharmaceuticals preparations, cosmetics, and as a food additive (E171). It contains microparticles fraction of nanoparticles (NPs) which can be absorbed systemically by humans after ingestion. Increasing concern has been aroused about the impact oral exposure to TiO2 NPs from dietary non-dietary sources on human health. In spite several toxicological studies conducted recent years, solid risk assessment E171 not satisfactorily achieved. We...
During the last 250 years, level of exposure to combustion-derived particles raised dramatically in western countries, leading increased particle loads ambient air. Among environmental particles, diesel exhaust particulate matter (DEPM) plays a special role because its omnipresence and reported effects on human health. recent possible link between air pollution progression atherosclerosis is recognized. A central effect DEPM their impact endothelium, especially alveolar barrier. In present...
Extracellular vesicles (EVs) are of interest for a wide variety biomedical applications. A major limitation the clinical use EVs is lack standardized methods fast and reproducible separation subsequent detection EV subpopulations from biofluids, as well their storage. To advance this application area, fluorescence-based characterization technologies with single-EV resolution, such high-sensitivity flow cytometry (HS-FCM), powerful to allow assessment fractionation storage conditions....
To characterise a biorelevant simulated lung fluid (SLF) based on the composition of human respiratory tract lining fluid. SLF was compared to other media which have been utilized as simulants in terms structure, biocompatibility and performance inhalation biopharmaceutical assays. The structure investigated using cryo-transmission electron microscopy, photon correlation spectroscopy Langmuir isotherms. Biocompatibility with A549 alveolar epithelial cells determined by MTT assay,...
Food-grade TiO2 (E171 in the EU) is widely used as a coloring agent foodstuffs, including sweets. Chronic dietary exposure raises concerns for human health due to proinflammatory properties and ability induce promote preneoplastic lesions rodent gut. Characterization of intestinal uptake essential assessing risk humans. We studied vivo gut absorption kinetics fasted mice orally given single dose (40 mg/kg) assess apical surfaces absorb particles when available without entrapment bolus. The...
The present study aimed to evaluate the potential differences in biological effects of two types spherical silver particles 20 and 200 nm (Ag20 Ag200), PVP-coated nanowires (AgNWs) with a diameter 50 length up μm, using complex 3D model representative for alveolar barrier cultured at air-liquid interface (ALI). was exposed 0.05, 0.5 5 μg/cm2 test compounds ALI state-of-the-art exposure system (Vitrocell™Cloud System). Endpoints related oxidative stress induction, anti-oxidant defence...