A5053105927- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Virus-based gene therapy research
- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- Immune cells in cancer
- Cutaneous Melanoma Detection and Management
- Esophageal Cancer Research and Treatment
- Prostate Cancer Treatment and Research
- HER2/EGFR in Cancer Research
- vaccines and immunoinformatics approaches
- Cervical Cancer and HPV Research
- Gastric Cancer Management and Outcomes
- Cancer Cells and Metastasis
- Microbial Inactivation Methods
- Cell Adhesion Molecules Research
- Gastrointestinal Tumor Research and Treatment
- Endometrial and Cervical Cancer Treatments
- interferon and immune responses
- Reproductive System and Pregnancy
- Respiratory viral infections research
- Cancer Research and Treatments
Vrije Universiteit Amsterdam
2012-2025
Amsterdam University Medical Centers
2018-2025
Cancer Center Amsterdam
2015-2024
Amsterdam UMC Location Vrije Universiteit Amsterdam
2014-2024
University Medical Center
2006-2024
Oncode Institute
1999-2024
Amsterdam Institute for Addiction Research
2023
John Wiley & Sons (United States)
2018-2021
Christ University
2018-2021
Hudson Institute
2018-2021
Noncoding regulatory microRNAs (miRNAs) of cellular and viral origin control gene expression by repressing the translation mRNAs into protein. Interestingly, miRNAs are secreted actively through small vesicles called "exosomes" that protect them from degradation RNases, suggesting these may function outside cell in which they were produced. Here we demonstrate EBV-infected cells transferred to act uninfected recipient cells. Using a quantitative RT-PCR approach, mature EBV-encoded B...
Abstract PD-L1 immunohistochemistry correlates only moderately with patient survival and response to PD-(L)1 treatment. Heterogeneity of tumor expression might limit the predictive value small biopsies. Here we show that PD-1 can be quantified non-invasively using PET-CT in patients non-small-cell lung cancer. Whole body reveals significant tracer uptake heterogeneity both between patients, as well within different lesions.
Programmed death-ligand 1 (PD-L1) is expressed in various immune cells and tumor cells, able to bind PD-1 on T lymphocytes, thereby inhibiting their function. At present, the PD-1/PD-L1 axis a major immunotherapeutic target for checkpoint inhibition cancer types, but information clinical significance of PD-L1 expression cervical largely lacking. Here, we studied paraffin-embedded samples from two cohorts patients with cancer: primary cohort I (squamous cell carcinoma, n=156 adenocarcinoma,...
The CROSS trial established neoadjuvant chemoradiotherapy (nCRT) for patients with resectable esophageal adenocarcinoma (rEAC). In the PERFECT trial, we investigated feasibility and efficacy of nCRT combined programmed-death ligand-1 (PD-L1) inhibition rEAC.Patients rEAC received according to regimen five cycles atezolizumab (1,200 mg). primary endpoint was administering in ≥75% patients. A propensity score-matched cohort used compare pathologic response, overall survival, progression-free...
Stereotactic ablative radiotherapy (SABR) has emerged as a new treatment option for patients with oligometastatic disease. SABR delivers precise, high-dose, hypofractionated radiotherapy, and achieves excellent rates of local control primary tumors or metastases. A recent randomized phase II trial evaluated in group small burden disease (mostly 1–3 metastatic lesions), found that was associated benefits progression-free survival overall survival. The goal this III is to assess the impact...
Production of immunosuppressive factors is one the mechanisms by which tumors evade immunosurveillance. Soluble hampering dendritic cell (DC) development have recently been identified in culture supernatants derived from tumor lines. In this study, we investigated presence such 24-h freshly excised solid human (colon, breast, renal carcinoma, and melanoma). While primary tumor-derived supernatant (TDSN) profoundly hampered vitro DC differentiation CD14(+) plastic-adherent monocytes or...
Regulatory T cell (Treg)-mediated immunosuppression is considered a major obstacle for successful cancer immunotherapy. The association between clinical outcome and Tregs being studied extensively in trials, but unfortunately, no consensus has been reached about (a) the markers (b) gating strategy required to define human this context, making it difficult draw final conclusions. Therefore, we have organized an international workshop on detection functional testing of with leading experts...
Abstract Purpose: Human osteosarcoma is a genetically heterogeneous bone malignancy with poor prognosis despite the employment of aggressive chemotherapy regimens. Because druggable driver mutations have not been established, dissecting interactions between cells and supporting stroma may provide insights into novel therapeutic targets. Experimental Design: By using bioluminescent orthotopic xenograft mouse model osteosarcoma, we evaluated effect tumor extracellular vesicle (EV)–educated...
Complex interactions between DNA herpesviruses and host factors determine the establishment of a life-long asymptomatic latent infection. The lymphotropic Epstein-Barr virus (EBV) seems to avoid recognition by innate sensors despite massive transcription immunostimulatory small RNAs (EBV-EBERs). Here we demonstrate that in latently infected B cells, EBER1 transcripts interact with lupus antigen (La) ribonucleoprotein, avoiding cytoplasmic RNA sensors. However, coculture experiments observed...
Purpose To (a) investigate the safety of percutaneous irreversible electroporation (IRE) for locally advanced pancreatic cancer and (b) evaluate quality life (QOL), pain perception, efficacy in terms time to local progression, event-free survival, overall survival (OS). Materials Methods The study was approved by review board (NL42888.029.13). All patients provided written informed consent participation, ablation procedure, data usage. Between January 2014 June 2015, 25 with histologically...
Background Patients with locally advanced pancreatic cancer have a dismal prognosis, median overall survival (OS) of 12–14 months systemic therapies. Irreversible electroporation (IRE), nonthermal ablative technique, may prolong patients cancer. Purpose To investigate the safety and efficacy percutaneous IRE for recurring in prospective phase II trial. Materials Methods Between December 2012 September 2017, participants or postresection local recurrence were prospectively treated CT-guided...
Abstract A better understanding of the microenvironment in relation to lymph node metastasis is essential for development effective immunotherapeutic strategies against cervical cancer. In present study, we investigated tumor-draining nodes patients with cancer by comprehensive flow cytometry–based phenotyping and enumeration immune-cell subsets tumor-negative (LN−, n = 20) versus tumor-positive (LN+, 8), study cytokine release profiles (n 4 both LN− LN+). We found significantly lower CD4+...
Abstract Background A recent randomized phase II trial evaluated stereotactic ablative radiotherapy (SABR) in a group of patients with small burden oligometastatic disease (mostly 1–3 metastatic lesions), and found that SABR was associated significant improvement progression-free survival trend to an overall benefit, supporting progression III trials. Methods Two hundred ninety-seven will be 1:2 ratio between the control arm (consisting standard care [SOC] palliative-intent treatments), SOC...