A5005119540- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Immune Response and Inflammation
- CAR-T cell therapy research
- Glycosylation and Glycoproteins Research
- Diabetes and associated disorders
- Cell Adhesion Molecules Research
- Immunodeficiency and Autoimmune Disorders
- Cancer Immunotherapy and Biomarkers
- Cytokine Signaling Pathways and Interactions
- Atherosclerosis and Cardiovascular Diseases
- IL-33, ST2, and ILC Pathways
- RNA Interference and Gene Delivery
- Galectins and Cancer Biology
- Toxin Mechanisms and Immunotoxins
- Neuroinflammation and Neurodegeneration Mechanisms
- Helicobacter pylori-related gastroenterology studies
- Systemic Lupus Erythematosus Research
- Macrophage Migration Inhibitory Factor
- Immune cells in cancer
- Transgenic Plants and Applications
- Cytomegalovirus and herpesvirus research
- T-cell and Retrovirus Studies
National Institute of Allergy and Infectious Diseases
2016-2025
National Institutes of Health
2016-2025
The Ohio State University
2023
Office of Extramural Research
2009-2015
National Institute of General Medical Sciences
2013
University of Oklahoma Health Sciences Center
2012
Mayo Clinic
2012
New Huadu Business School
2012
National Heart Lung and Blood Institute
1986-2009
Laboratory of Molecular Genetics
1988-2008
Peripheral tolerance may be maintained by a population of regulatory/suppressor T cells that prevent the activation autoreactive recognizing tissue-specific antigens. We have previously shown CD4+CD25+ represent unique suppressor can both initiation organ-specific autoimmune disease after day 3 thymectomy and effector function cloned autoantigen-specific CD4+ cells. To analyze mechanism action these cells, we established an in vitro model system mimics vivo. Purified failed to proliferate...
Helios, a member of the Ikaros transcription factor family, is preferentially expressed at mRNA level by regulatory T cells (Treg cells). We evaluated Helios protein expression using newly generated mAb and demonstrated that it in all thymocytes double negative 2 stage thymic development. Although was 100% CD4(+)CD8(-)Foxp3(+) thymocytes, its peripheral lymphoid tissues restricted to subpopulation ( approximately 70%) Foxp3(+) mice humans. Neither mouse nor human naive induced express Foxp3...
Abstract CD4+CD25+ T cells represent a unique population of “professional” suppressor that prevent induction organ-specific autoimmune disease. In vitro, were anergic to simulation via the TCR and when cultured with CD4+CD25− cells, markedly suppressed polyclonal cell proliferation by specifically inhibiting production IL-2. Suppression was cytokine independent, contact dependent, required activation suppressors their TCR. Further characterization demonstrated they do not contain memory or...
Antigen activation of DNA synthesis in immune thymus-derived lymphocytes guinea pigs requires the cooperation macrophages and lymphocytes. We have investigated role histocompatibility determinants this macrophage-lymphocyte interaction using cells from inbred strain 2 13 pigs. The data demonstrate that efficient presentation macrophage-associated antigen to lymphocyte identity between macrophage at some portion major complex. failure allogeneic effectively initiate proliferation was not...
Thymectomy of susceptible strains mice on day 3 life results in a spectrum organ-specific autoimmunity that can be prevented by reconstitution the thymectomized animals early with normal adult lymphocytes. The effectors and suppressors this model have been convincingly shown to CD4+ T cells. It has demonstrated recently regulatory cells prevent disease coexpress CD25. We further characterized population CD4+CD25+ immunoregulatory they suppress not only induction post-thymectomy, but also...
We have previously demonstrated that the inhibitory effects of IL-10 on ConA-induced T cell proliferation or IL-2 production by resting murine cells were only observed when macrophages, but not activated B cells, dendritic L used as accessory cells. To further elucidate mechanism action inhibition macrophage costimulatory activity, we a system in which macrophages can develop into effective costimulator and effect this process be studied absence After fixation, no activity for soluble...
CD4(+)CD25(+) regulatory T cells inhibit organ-specific autoimmune diseases induced by CD4(+)CD25(-) and are potent suppressors of cell activation in vitro. We demonstrate that also suppress both proliferation IFN-gamma production CD8(+) either polyclonal or Ag-specific stimuli. the responders inhibiting IL-2 up-regulation IL-2Ralpha-chain (CD25) expression. Suppression is mediated via a T-T interaction as activated responses TCR-transgenic stimulated with soluble peptide-MHC class I...
Abstract Langerhans cells (LC) constitute a morphologically well-characterized minor subpopulation of the mammalian epidermis whose functional role is still matter conjecture. The hypothesis that LC represent an epidermal equivalent to monocyte-macrophage-histiocyte series supported by recent observations in humans and guinea pigs are only express Fc-IgG receptors, C3 Ia antigens. Using inbred strain 2 13 pigs, we investigated this study whether can mediate same immunologic functions as...
CD4(+)CD25(+) regulatory T cells inhibit organ-specific autoimmune diseases induced by CD4(+)CD25(-) and are potent suppressors of cell activation in vitro. Their mechanism suppression remains unknown, but most vitro studies suggest that it is contact-dependent cytokine independent. The role TGF-beta1 suppressor function unclear. While have failed to reverse with anti-transforming growth factor (TGF)-beta1 vitro, one recent study has reported express surface can be completely abrogated high...
A number of recent studies have suggested that the main functional role product immune response (Ir) genes is in process antigen recognition by T lymphocyte. The observation accompanying report interaction macrophage-associated with lymphocytes requires both cells share histocompatibility antigens raised question as to whether macrophage played a genetic control or even if were primary cell which Ir gene expressed. In current study, parental macrophages pulsed an antigen, controlled lacking...
The properties and outcome of an immune response are best predicted by the lymphokine phenotype responding T cells. Cytokines produced CD4+ helper type 1 (Th1) cells mediate delayed hypersensitivity (DTH) inflammatory responses, whereas cytokines Th2 cell functions for antibody production. To determine whether induction Th2-like would modulate response, interleukin 4 (IL-4) was administered to animals with experimental allergic encephalomyelitis (EAE), a prototypic autoimmune disease...
CD4(+)CD25(+) T cells are potent immunoregulatory that suppress TCR-induced proliferation of CD4 and CD8 in vitro by a cell contact-dependent mechanism. Addition IL-2 or anti-CD28 abrogates CD4(+)CD25(+)-mediated suppression has been assumed to "break suppression." We examined mRNA quantitative PCR cocultures mouse CD4(+)CD25(-) cells. Although gene transcription was inhibited the presence absence exogenous IL-2, addition stimulated endogenous production. Surprisingly, also restored...