A5083692791- Extracellular vesicles in disease
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Protease and Inhibitor Mechanisms
- Immunotherapy and Immune Responses
- Bacterial Infections and Vaccines
- Molecular Biology Techniques and Applications
- RNA Research and Splicing
- Asthma and respiratory diseases
- Mast cells and histamine
- Nanopore and Nanochannel Transport Studies
- Cell Adhesion Molecules Research
- Signaling Pathways in Disease
- Ocular Oncology and Treatments
- Machine Learning and ELM
- Cellular transport and secretion
- Amoebic Infections and Treatments
- Click Chemistry and Applications
- Synthesis and biological activity
- Tissue Engineering and Regenerative Medicine
- Parvovirus B19 Infection Studies
- Cell death mechanisms and regulation
- Ubiquitin and proteasome pathways
National Institutes of Health
2021-2023
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2021-2023
University of Gothenburg
2014-2021
Sahlgrenska University Hospital
2019-2021
National Centre for Cell Science
2010-2017
Savitribai Phule Pune University
2017
To dissect therapeutic mechanisms of transplanted stem cells and develop exosome-based nanotherapeutics in treating autoimmune neurodegenerative diseases, we assessed the effect exosomes secreted from human mesenchymal (MSCs) multiple sclerosis using an experimental encephalomyelitis (EAE) mouse model. We found that intravenous administration produced by MSCs stimulated IFNγ (IFNγ-Exo) (i) reduced mean clinical score EAE mice compared to PBS control, (ii) demyelination, (iii) decreased...
Extracellular vesicles (EVs), including the nano-sized exosomes, have capacity to transfer multiple functional molecules between cells. In cell culture experiments, fetal bovine serum (FBS) is often used supplement medium as a nutrient, but it important know that FBS also contain significant quantities of EVs. The aim current study was determine whether EVs can influence cultured phenotype, and secondly efficiency FBS-EV elimination protocols. Firstly, had not been depleted induced migratory...
Extracellular vesicles have the capacity to transfer lipids, proteins, and nucleic acids between cells, thereby influencing recipient cell's phenotype. While role of RNAs in EVs has been extensively studied, function DNA remains elusive. Here, we distinguished novel heterogeneous subpopulations small extracellular (sEVs) based on their content topology. Low- high-density sEV subsets from a human mast cell line (HMC-1) an erythroleukemic (TF-1) were separated using high-resolution iodixanol...
Cells secrete extracellular RNA (exRNA) to their surrounding environment and exRNA has been found in many body fluids such as blood, breast milk cerebrospinal fluid. However, there are conflicting results regarding the nature of exRNA. Here, we have separated 2 distinct profiles released by mast cells, here termed high-density (HD) low-density (LD) The both fractions was characterized microarray next-generation sequencing. Both contained mRNA miRNA, mRNAs LD correlated closely with cellular...
Extracellular vesicles such as exosomes convey biological messages between cells, either by surface-to-surface interaction or shuttling of bioactive molecules to a recipient cell's cytoplasm. Here we show that released mast cells harbour both active and latent transforming growth factor β-1 (TGFβ-1) on their surfaces. The form TGFβ-1 is associated with the via heparinase-II pH-sensitive elements. These traffic endocytic compartment human mesenchymal stem (MSCs) within 60 min exposure....
Abstract Metastatic uveal melanoma is less well understood than its primary counterpart, has a distinct biology compared to skin melanoma, and lacks effective treatments. Here we genomically profile metastatic tumors infiltrating lymphocytes. BAP1 alterations are overrepresented found in 29/32 of cases. Reintroducing functional allele into deficient patient-derived cell line, reveals broad shift towards transcriptomic subtype previously associated with better prognosis the disease. One...
Exosomes are nano-sized extracellular vesicles participating in cell-to-cell communication both health and disease. However, the knowledge about functions molecular composition of exosomes upper airways is limited. The aim current study was therefore to determine whether nasal can influence inflammatory cells establish proteome lavage fluid-derived healthy subjects, as well its alterations individuals with chronic airway diseases [asthma rhinosinusitis (CRS)].Nasal fluid collected from 14 15...
Human cells release nano-sized vesicles called exosomes, containing mRNA, miRNA and specific proteins. Exosomes from one cell can be taken up by another cell, which is a recently discovered cell-to-cell communication mechanism. Also, exosomes different types of cancer cells, but the potential functional effects mast on tumor remain unknown. were isolated human line, HMC-1, uptake PKH67-labelled lung epithelial A549, was examined using flow cytometry fluorescence microscopy. The RNA cargo...
Sepsis remains a source of high mortality in hospitalized patients despite proper antibiotic approaches. Encouragingly, mesenchymal stromal cells (MSCs) and their produced extracellular vesicles (EVs) have been shown to elicit anti-inflammatory effects multiple inflammatory conditions including sepsis. However, EVs are generally released from mammalian relatively low amounts, high-yield isolation is still challenging due complicated procedure. To get over these limitations, very similar can...
Abstract Cell culture‐conditioned medium (CCM) is a valuable source of extracellular vesicles (EVs) for basic scientific, therapeutic and diagnostic applications. culturing parameters affect the biochemical composition, release possibly function CCM‐derived EVs (CCM‐EV). The CCM‐EV task force Rigor Standardization Subcommittee International Society Extracellular Vesicles aims to identify relevant cell parameters, describe their effects based on current knowledge, recommend reporting...
Accurate concentration determination of subpopulations extracellular vesicles (EVs), such as exosomes, is importance both in the context understanding their fundamental biological role and potentially using them disease biomarkers. In principle, this can be achieved by measuring rate diffusion-limited mass uptake to a sensor surface modified with receptor designed only bind subpopulation interest. However, significant error introduced if targeted EV has size, thus hydrodynamic diffusion...
Exosomes are small vesicles that secreted from cells to dispose of undegraded materials and mediate intercellular communication. A major source exosomes is intraluminal within multivesicular endosomes undergo exocytic fusion with the plasma membrane. An alternative fate lysosomes, resulting in degradation vesicles. The factors determine whether fuse membrane or lysosomes unknown. In this study, we show impairment endolysosomal by disruption a pathway involving BLOC-one-related complex...
Sepsis induced cardiac dysfunction (SIC) is a severe complication to sepsis which significantly worsens patient outcomes. It known that bacteria have the capacity release outer membrane vesicles (OMVs), are nano-sized bilayered composed of lipids and proteins, can induce fatal inflammatory response. The aim this study was determine whether OMVs from uropathogenic Escherichia coli strain dysfunction, elucidate any mechanisms involved. irregular Ca2+ oscillations with decreased frequency in...
Abstract Background In the airways, mast cells are present in close vicinity to epithelial cells, and they can interact with each other via multiple factors, including extracellular vesicles (EVs). Mast cell-derived EVs have a large repertoire of cargos, proteins RNA, as well surface DNA. this study, we hypothesized that these induce mesenchymal transition (EMT) airway cells. Methods in-vitro study systematically determined effects on A549 We changes induced by at both RNA protein levels....
Extracellular vesicles (EVs) carry multiple bioactive molecules, including proteins and nucleic acids. Cell-free, extracellular DNA has been reported to be present in cell cultures body fluids, but its relative location relation EVs not described. This study demonstrates that DNase sensitive acids are on the surface of EV isolates. Association EV-DNA was revealed by increase zeta potential particle number upon treatment. Additionally, Cells exposed with associated DNA, show presence...
Human cells release nano-sized vesicles called exosomes, containing mRNA, miRNA and specific proteins. Exosomes from one cell can be taken up by another cell, which is a recently discovered cell-to-cell communication mechanism. Also, exosomes different types of cancer cells, but the potential functional effects mast on tumor remain unknown. were isolated human line, HMC-1, uptake PKH67-labelled lung epithelial A549, was examined using flow cytometry fluorescence microscopy. The RNA cargo...
Extracellular vesicles (EVs) are generating a growing interest because of the key roles they play in various biological processes and their potential use as biomarkers clinical diagnostics efficient carriers drug-delivery gene-therapy applications. Their full exploitation, however, depends critically on possibility to classify them into different subpopulations, task that turn relies means identify unique biomolecular physical signatures. Because large heterogeneity EV samples, such...
Sodium valproate (VPA) has been recently identified as a selective class I histone deacetylase (HDAC) inhibitor and explored for its potential an anti-cancer agent. The properties of VPA are generally attributed to HDAC inhibitory activity indicating clear overlap these two actions, but the underlying mechanisms anti-tumor effects not clearly elucidated. present study aimed delineate molecular mechanism in potentiating cytotoxic drugs with focus on inhibition activity. Using human...
The objective of this study was to examine the combined effect Interferon-gamma (IFN-γ) and Tumor Necrosis factor-alpha (TNF-α) on cytotoxicity expression prostate apoptosis response-4 (Par-4) Par-4 interacting proteins B-cell lymphoma (Bcl-2), nuclear factor kappa-light-chain-enhancer activated B cells/p65 subunit (NF-κB/p65), Ak mouse strain thymoma (Akt) in human neuroblastoma (NB) cells. Materials methods included cell lines-SK-N-MC, SK-N-SH, SH-SY5Y, which were treated with IFN-γ TNF-α...
Studying the proteomes of tissue-derived extracellular vesicles (EVs) can lead to identification biomarkers disease and provide a better understanding cell-to-cell communication in both healthy diseased tissue. The aim this study was apply our previously established EV isolation protocol mouse lungs order determine changes lung EVs during allergen-induced eosinophilic airway inflammation. A model for allergic inflammation used by sensitizing mice intraperitoneal with ovalbumin (OVA), one...
2D and 3D quantitative structure–activity relationship studies have been carried out for establishing a correlation between the structural properties of benzyl urea derivatives their anti-tumour activities. From this correlation, new chemical entities were designed, activity absorption, distribution, metabolism, excretion, toxicity also predicted. Finally, most promising compounds from these screening synthesized biologically evaluated anti-cancer properties. Compound 1-(2,...
Abstract Extracellular vesicles (EVs) convey biological messages between cells, either by surface-to-surface interaction, or shuttling of bioactive molecules to a recipient cell cytoplasm. Here we show that EVs released human primary mast cells transformed (HMC1), carry TGFβ-1 on their surface. EV-associated enhance the migratory activity mesenchymal stem (MSCs) compared free TGFβ-1, as both knockdown TGFβ, TGFβ-antibody, attenuate effect. The MSCs respond increasing matrix...