A5069926611- Prenatal Screening and Diagnostics
- Cancer Genomics and Diagnostics
- Pregnancy and preeclampsia studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Birth, Development, and Health
- Single-cell and spatial transcriptomics
- Cancer Cells and Metastasis
- Oral microbiology and periodontitis research
- Rheumatoid Arthritis Research and Therapies
- Parvovirus B19 Infection Studies
- Preterm Birth and Chorioamnionitis
- Acute Kidney Injury Research
- Immune Response and Inflammation
- Fetal and Pediatric Neurological Disorders
- Salivary Gland Disorders and Functions
- Extracellular vesicles in disease
- Renal and related cancers
- Cancer, Hypoxia, and Metabolism
- Sarcoma Diagnosis and Treatment
- Organ Donation and Transplantation
- Neonatal Respiratory Health Research
- Reproductive System and Pregnancy
- Testicular diseases and treatments
- Metastasis and carcinoma case studies
- Pharmacological Effects and Toxicity Studies
Comenius University Bratislava
2015-2024
Beth Israel Deaconess Medical Center
2019-2023
Harvard University
2019-2023
American Association for the Surgery of Trauma
2020
Seoul National University
2020
Harbin Medical University
2020
Massachusetts Institute of Technology
2020
Biomedical Research Center of the Slovak Academy of Sciences
2016
Slovak Academy of Sciences
2014-2015
National Cancer Institute
2013
This study aimed to evaluate the amniotic fluid protein profiles and intensity of intraamniotic inflammatory response Ureaplasma spp. other bacteria, using multiplex xMAP technology.A retrospective cohort was undertaken in Department Obstetrics Gynecology, University Hospital Hradec Kralove, Czech Republic. A total 145 pregnant women with preterm prelabor rupture membranes between gestational age 24+0 36+6 weeks were included study. Amniocenteses performed. The presence bacteria evaluated...
Cancer is a risk factor for venous thromboembolism (VTE) and plasma d-dimer (DD) tissue (TF) are established VTE associated markers. Circulating tumor cells (CTCs) with the of in metastatic breast cancer. This study aimed to correlate CTCs, blood coagulation urokinase plasminogen activator (uPA) system primary cancer (PBC) patients. prospective included 116 PBC patients treated by surgery. CTCs were detected quantitative RT-PCR assay expression epithelial (CK19) or epithelial-mesenchymal...
Several recent studies have shown that liver injury is associated with the release of DNA from hepatocytes. This stimulates innate immunity and induces sterile inflammation, exacerbating damage. Similar mechanisms been described for acute renal injury. Deoxyribonuclease degrades cell-free can potentially prevent some induced tissue study analyzed effects thioacetamide-induced hepatorenal on plasma in rats. Plasma both nuclear mitochondrial origin was higher thioacetamide-treated animals....
Resveratrol is a natural polyphenol studied for its possible protective properties in inflammatory bowel diseases. Moreover, it has been shown to interact with estrogen receptors. In the present study, we aimed investigate diverse effects of resveratrol on female and male mice DSS-induced colitis. Thirty-seven C57BL/6 (21 16 male) were divided into three groups each sex. The first group received pure water (CTRL). other two 1.5% dextran sulfate sodium (DSS) induce colitis from which one was...
Trauma predisposes to systemic sterile inflammation (systemic inflammatory response syndrome) as well infection, but the mechanisms linking injury infection are poorly understood. Mitochondrial debris contains formyl peptides. These bind peptide receptor-1, trafficking neutrophils wounds, initiating syndrome, and wound healing. Bacterial peptides, however, also attract via receptor-1. Thus, mitochondrial peptides might suppress antimicrobial function. Also, receptor-1 blockade used mitigate...
Patients with ovarian cancer represent a heterogeneous population variable prognosis and response to chemotherapy. Plasma DNA has been shown have prognostic value in different types of including carcinoma. Whether total circulating DNA, which can be assessed much easier without knowing the tumor-specific mutations, similar informative is currently unknown. The aim this study was evaluate extracellular advanced cancer.This prospective included 67 patients (pts) treated 1st line paclitaxel...
BACKGROUND Trauma and sepsis both increase the risk for secondary infections. Injury mobilizes mitochondrial (MT) danger-associated molecular patterns (mtDAMPs) directly from cellular necrosis. It is unknown, however, whether can cause active MT release mtDAMPs released by might affect innate immunity. METHODS Mitochondrial human monocytes (Mo) was studied after LPS stimulation using electron microscopy fluorescent video-microscopy of adherent Mo Mito-Tracker Green (MTG) dye. Release MTG +...
Introduction: Rheumatoid arthritis (RA) is a chronic inflammatory disorder with high prevalence among middle-aged women. Collagen-induced (CIA) the most widely used animal model of RA, however, sex differences and long-term effects CIA in mice are poorly described literature. Aim: Therefore, present study aimed to analyze on joints both sexes describe potential differences. Materials methods: was induced DBA/1J by immunization bovine type II collagen complete Freund’s adjuvant. Saline...
As a main excretory organ, kidney is predisposed to direct/indirect injury. We addressed the potential nephrotoxic effects following expositions of healthy rats nanoparticle (NP) loads relevant humans in situation 100% bioavailability. Up 4 weeks after administration, single iv bolus oleate-coated ultra-small superparamagnetic iron oxide NPs (in dose 0.1%, 1.0% and 10.0% LD50) or TiO2 (1.0% did not elicit decline renal function, damage proximal tubules, alterations in: histology expression...
Objective: To determine amniotic fluid total antioxidant capacity (TAC), ferric-reducing power (FRAP) and thiobarbituric acid-reacting substances (TBARS), markers of oxidative stress, in pregnancies complicated by preterm prelabor rupture membranes (pPROM) their correlation to microbial invasion the cavity (MIAC) and/or histological chorioamnionitis (HCA).Methods: One-hundred thirty-eight women with singleton pPROM were included this study. Amniotic was collected transabdominal amniocentesis...
Abstract The non-invasive, flexible and easy sample collection makes saliva an interesting source of DNA for research diagnostic purposes. aim our study was to find the most suitable method biological material from oral cavity effective isolation technique further analytic applications. isolated swabs, Salivette saliva, whole samples collected with a commercial set scraping buccal cells. Phenol-chloroform extraction using silica membrane based kit were compared. Quantity bacterial human...
Abstract Detection of fetal sex based on DNA present in maternal plasma is already clinical use. Here we a case false positivity during the first trimester which may be attributable to vanishing twin. The presence Y ‐chromosome‐specific sequences used as marker indicate male fetus and absence female fetus. Fetal determination was conducted pregnant woman at gestational week 10. sample positive all triplicates. Ultrasonography 20 revealed sex. Analysis taken 22 indicated According recently...
Abstract Recent studies show that the salivary microbiome in subjects with obesity differ from those without obesity, but mechanism of interaction between composition and body weight is unclear. Herein we investigate this relation by analyzing saliva samples 35 adult patients undergoing bariatric surgery. Our aim was to describe changes during loss on an individual-specific level, elucidate effect surgery which has not been studied before. Analysis collected before 1 day after surgery, as...