A5016941274- Stress Responses and Cortisol
- Hormonal and reproductive studies
- Hypothalamic control of reproductive hormones
- Autism Spectrum Disorder Research
- Neuroendocrine regulation and behavior
- Estrogen and related hormone effects
- Cancer Genomics and Diagnostics
- Neonatal Respiratory Health Research
- Birth, Development, and Health
- Sexual Differentiation and Disorders
- Genetics and Neurodevelopmental Disorders
- Single-cell and spatial transcriptomics
- Neonatal and fetal brain pathology
- Biochemical effects in animals
- Congenital heart defects research
- Adipose Tissue and Metabolism
- Advanced biosensing and bioanalysis techniques
- Meat and Animal Product Quality
- Neurogenesis and neuroplasticity mechanisms
- Animal Genetics and Reproduction
- Cell Adhesion Molecules Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Gut microbiota and health
- Glioma Diagnosis and Treatment
- Genetics and Physical Performance
Comenius University Bratislava
2016-2025
Aging in women is associated with low estrogen, but also cognitive decline and affective disorders. Whether estrogen causally responsible for these behavioral symptoms not clear. Thus, we aimed to examine the role of estradiol anxiety-like behavior memory rats at middle age. Twelve-month old female underwent ovariectomy (OVX) or were treated 1mg/kg letrozole – an aromatase inhibitor. In half OVX females, 10µg/kg 17β-estradiol was supplemented daily four weeks. Vehicle-treated sham-operated...
Increasing evidence of sexual dimorphism in the pathophysiology metabolic complications caused by sex steroids is under investigation. The gut microbiota represents a complex microbial ecosystem involved energy metabolism, immune response, nutrition acquisition, and health host organisms. Gender-specific differences composition are present between females males. purpose this study was to use cross-sex fecal transplantation (FMT) for detection sex-dependent metabolic, hormonal, changes female...
Abstract Dysfunctional sensory systems, including altered olfactory function, have recently been reported in patients with autism spectrum disorder (ASD). Disturbances processing can potentially result from gamma-aminobutyric acid (GABA)ergic synaptic abnormalities. The specific molecular mechanism by which GABAergic transmission affects the system ASD remains unclear. Therefore, present study aimed to evaluate selected components of brain regions and primary neurons isolated Shank3...
Several recent studies have shown that liver injury is associated with the release of DNA from hepatocytes. This stimulates innate immunity and induces sterile inflammation, exacerbating damage. Similar mechanisms been described for acute renal injury. Deoxyribonuclease degrades cell-free can potentially prevent some induced tissue study analyzed effects thioacetamide-induced hepatorenal on plasma in rats. Plasma both nuclear mitochondrial origin was higher thioacetamide-treated animals....
Laboratory mice in standard laboratory cages, besides horizontal and vertical locomotor activity, spontaneously display cage-bar related activities such as cage-grid climbing. Although, grid-climbing activity is one of the major components spontaneous home-cage behavior mice, its exact role not fully understood. This study aimed to describe sex-differences coping with novelty concerning cage-climbing an observer-independent open field test. Adult both sexes (C57Bl/6NTac) underwent behavioral...
Introduction: Rheumatoid arthritis (RA) is a chronic inflammatory disorder with high prevalence among middle-aged women. Collagen-induced (CIA) the most widely used animal model of RA, however, sex differences and long-term effects CIA in mice are poorly described literature. Aim: Therefore, present study aimed to analyze on joints both sexes describe potential differences. Materials methods: was induced DBA/1J by immunization bovine type II collagen complete Freund’s adjuvant. Saline...
Abstract Autism spectrum disorder (ASD) is a group of neurodevelopmental disorders mainly characterized by deficient sociability and repetitive behaviors. Effective treatment for the core symptoms ASD still lacking. Behavioral interventions show limited effectiveness, while pharmacotherapy focuses on amelioration secondary symptomatology. Oxytocin (OXT) neuropeptide known its prosocial impact, making it candidate drug treatment. Its alleviating effect has been widely researched, but outcomes...
Summary Aromatase catalyzes the conversion of testosterone to estradiol and is involved in physiological effects sex hormones on brain function. Animal experiments have shown that aromatase inhibitor, letrozole, can induce anxiety young ovariectomized females are used as a model aging. Whether or not these would be similar intact middle‐aged animals unknown. The aim our study was analyze letrozole rats both sexes. Fifteen month old male female were treated daily with either vehicle for 2...
The aim of this study was investigate whether histopathological changes in the neurogenic region correlate with appropriate cognitive impairment experimental model radiation-induced brain injury.Adult male Wistar rats randomized into sham (0 Gy) and two groups (survived 30 100 days after treatment) received fractionated whole-brain irradiation (one 5 Gy fraction/week for four weeks) a total dose 20 gamma rays. Morris water maze testing, histochemistry, immunohistochemistry confocal...
Hypoxia is one of the major pathological factors affecting brain function. The aim present study was to describe effect neonatal hypobaric hypoxia on behavior rats and analyze its hippocampal neurodegeneration. Hypobaric at a simulated altitude 9000 m induced for hour in rat pups (PND7 PND9) both sexes. Subsequently, underwent behavioral testing PND25 PND35 using LABORAS apparatus assess spontaneous behavior. did not cause any morphological damage hippocampus rats. However, PND7 led less...
Abstract The collection and examination method of vaginal smears is the standard for determination ovulation or phases estrous cycle rodents used in research. However, this time consuming may not be amenable to continual monitoring a large number animals. Infrared thermography has recently emerged as noninvasive technique that requires relatively little handling body temperature been shown correlate with ocular surface temperature. This study aimed evaluate use thermographic identification...
Autism Spectrum Disorder (ASD) is a group of neurodevelopmental disorders with heterogeneous symptomatology. Arguably, the most pervasive shortfall ASD are deficits in sociability and animal models disorder expected to exhibit such impairments. The widely utilized behavioral task for assessing rodents Three-Chamber Social Interaction Test (SIT). However, SIT has been yielding inconsistent results social interaction behavior across different rodent ASD, which could be pointing suboptimal...
Concentration of extracellular DNA (ecDNA) in plasma septic patients is higher comparison to healthy controls and associated with worse prognosis intensive care patients. Decrease ecDNA by treatment deoxyribonuclease (DNase) showed have beneficial effects animal models sepsis. A previously published study that timing DNase application crucial for the effect DNase. No monitored dynamics during sepsis detail yet. The aim our was describe early but also activity a mouse model Sepsis induced...
In men, aging is accompanied by a gradual decline in androgen secretion. Studies suggest beneficial effects of endogenous and exogenous testosterone on affective behavior cognitive functions. The aim this study was to describe behavioral sex differences analyze the long-term deficiency aged male rats. Thirty-months old rats divided into three groups (males, females males gonadectomized as young adults) underwent battery tests assessing locomotor activity, anxiety, memory, anhedonia,...
Abstract The treatment of testicular cancer includes unilateral orchiectomy and chemotherapy is curative for most patients. However, observational studies revealed an association with depression, anxiety cognitive impairment. It unclear whether these side effects are caused by chemotherapy, hemicastration or the disease itself. aim our study was to analyse behavioural in adult male mice. animals were randomly divided into four groups – control, chemotherapy. After that included three cycles...