Urinary tract infections (UTI) are common types of bacterial infection in children. UTI treatment is aimed to prevent complications including hypertension, proteinuria, and progression chronic kidney disease. Activated neutrophils release chromatin-based structures associated with antimicrobial proteins called neutrophil extracellular traps (NETs). We describe the role NET-associated markers children as well NETs formation a mouse model UTI.Markers DNA (ecDNA), myeloperoxidase (MPO)...

Kidney disease is a worldwide health and economic burden, with rising prevalence. The search for biomarkers earlier more effective screening monitoring needed. Oxidative stress has been linked to both, acute kidney injury (AKI) chronic (CKD). aim of our study was investigate whether the concentrations systemic markers oxidative antioxidant status are affected by AKI CKD, identify potential biomarkers. In adult male Wistar rats, induced bilateral nephrectomy, CKD 5/6 nephrectomy. Blood...

Oxidative stress markers are usually measured in plasma, a stable environment for biomarkers. Blood collection is invasive, but the use of alternative biofluids limited, due to high variability. In this study, we aimed establish reference values oxidative urine and saliva adult, healthy mice identify some sources Samples were obtained from 41 female 37 male CD-1 strain, aged 95-480 days, weighing 21-55 grams. Reference ranges TBARS (thiobarbituric acid reactive substances), AOPP (advanced...

Decreased renal function due to chronic kidney disease (CKD) is associated with anxiety and cognitive decline. Although these mental disorders are often obvious in late stage patients, they might be unnoticeable or neglected early stages of the CKD development. Associations between dysfunction have been indicated by studies performed mainly patients undergoing dialysis, which itself represents a stress decreased quality life. However, experimental causal scarce. Our aim was investigate...

Abstract Extracellular DNA (ecDNA) in plasma is a non-specific biomarker of tissue damage. Urinary ecDNA, especially mitochondrial origin, potential non-invasive kidney Despite prominent damage, ecDNA has not yet been comprehensively analysed acute injury (AKI). We different fractions i.e. total, nuclear and mitochondrial, urine children, animal models AKI. also the activity deoxyribonuclease (DNase), which contributes to degradation ecDNA. Patients with AKI had higher total both, (sixfold...

Abstract Saliva can be used as an alternative diagnostic fluid enabling easy and non-invasive disease monitoring. Urea creatinine measured in saliva both were shown to increased renal failure. However, the dynamics of these markers during development kidney diseases is unknown. We aimed describe salivary urea various animal models acute injury (AKI) chronic (CKD) patients with different stages AKI or CKD. Ninety Wistar rats underwent bilateral nephrectomy (BNX), ischemia–reperfusion (IRI)...

Despite the fact that saliva contains measurable concentrations of urea and creatinine, it is not widely used in clinical nephrology. One reasons high inter‐ intra‐individual variability salivary markers kidney function. We hypothesized gingival bleeding patients with periodontitis could contribute to this by increasing concentration or creatinine. Samples were collected from 25 29 healthy controls. In addition, samples five volunteers artificially contaminated blood. The urea, but was more...

Plasma creatinine and urea are commonly used markers of kidney function in both acute chronic renal failure. The needed repeated blood collection is associated with pain, stress might lead to infections. Saliva has the potential be a non-invasive alternative diagnostic fluid. use saliva clinical practice limited, since many factors affect concentration salivary biomarkers. aim our study was analyze animal models disease. Bilateral nephrectomy adenine nephropathy were induced adult male mice....

Type 2 diabetes mellitus has been referred to as being closely related oxidative stress, which may affect brain functions and glucose metabolism due its high metabolic activity lipid-rich content. Na,K-ATPase is an essential enzyme maintaining intracellular homeostasis, with properties that can sensitively mirror various pathophysiological conditions such diabetes. The goal of this study was determine stress markers well activities in the cerebellum Zucker diabetic fatty (ZDF) rats depending...

Periodontitis is a chronic inflammatory disease. We have previously shown that salivary DNA higher in patients with periodontitis. Neutrophil extracellular traps (NETs) are involved the pathogenesis of diseases. The objective this case-control study was to compare periodontitis and healthy controls regarding concentrations NET components. Unstimulated saliva samples were collected from 49 71 before an oral examination. Salivary isolated quantified fluorometrically using PCR. NET-associated...

Concentration of extracellular DNA (ecDNA) in plasma septic patients is higher comparison to healthy controls and associated with worse prognosis intensive care patients. Decrease ecDNA by treatment deoxyribonuclease (DNase) showed have beneficial effects animal models sepsis. A previously published study that timing DNase application crucial for the effect DNase. No monitored dynamics during sepsis detail yet. The aim our was describe early but also activity a mouse model Sepsis induced...

Early and reliable markers of acute kidney injury (AKI) are essential. One such candidate marker tissue damage is extracellular DNA (ecDNA). The aim our present study to describe the unknown dynamics ecDNA in an animal model AKI. Glycerol-induced nephropathy was used AKI adult male Wistar rats (n = 93). Blood urine samples were collected 1, 3, 24 h after induction. Total its sub-cellular origin assessed. In plasma, total nuclear significantly increased group already 1 (160% 270%,...

Abstract Background Anorexia nervosa (AN) is a serious psychosomatic disorder with unclear pathomechanisms. Metabolic dysregulation associated disruption of redox homeostasis that might play pivotal role in the development AN. The aim our study was to assess oxidative status and carbonyl stress plasma, urine saliva patients AN healthy controls. Methods Plasma, spot urine, were collected from 111 girls (aged 10 18 years) 29 age-matched Markers antioxidant measured using spectrophotometric...

Kidney and uropoetic system diseases represent a major social, economic health burden. This is mainly because early diagnosis of kidney dysfunction currently unavailable, since the current markers are often reliably increased only after advanced progression renal diseases. Recently, circulating nucleosomes, DNA numerous forms RNA have been detected in human biological fluids, such as plasma, urine, saliva, breast milk. Although their functions remain mostly unknown, they attractive potential...

Extracellular DNA (ecDNA) is a promising candidate marker for the early diagnosis and monitoring of urinary tract infections (UTIs). The aim our study to describe dynamics ecDNA in plasma urine patients with urosepsis as well mouse model UTI. Samples blood were collected from adult UTIs obstructive uropathy (n = 36) during first 3 days at hospital follow-up. Bacterial burden evaluated UTI 26) baseline; 24, 48, 72 h after induction; 7 induction. did not change urosepsis, but DNase activity...

Representative urine collection that respects the standards of animal welfare is still an issue in experimental nephrology. The commonly used metabolic cages induce stress rodents. In mice, volume collected sometimes insufficient for further analysis. aim this experiment was to analyse effects time day, temperature and 2%, 5% or 10% sucrose solutions on diuresis, weight change liquid intake adult mice placed collection. Mice were 12 h during day night at standard ambient (22℃) thermoneutral...

Extracellular DNA (ecDNA) is a potential marker and predictor in several inflammatory diseases. Periodontitis, chronic disease, associated with epithelial cell death could lead to release of DNA. Our aim was analyze salivary concentration deoxyribonuclease (DNase) activity periodontitis patients. We hypothesized that ecDNA will be higher than controls serve as severity. Samples saliva were collected from 25 patients 29 age-matched controls. quantified fluorometrically whole saliva, well...

Neutrofily sú centrálnou zložkou vrodeného imunitného systému. V posledných rokoch si získali značnú pozornosť vďaka novoobjaveným biologickým efektorovým funkciám a ich zapojeniu do rôznych patologických stavov. predstavujú prvú líniu obrany systému pôsobia ako fyzická chemická bariéra proti patogénom. Aktivované neutrofily môžu okrem iného uvoľňovať svoj obsah - dekonde nzovaný chromatín spolu s antimikrobiálnymi granulárnymi proteínmi, vytvárať tak tzv. neutrofilové extracelulárne pasce...

Searchable abstracts of presentations at key conferences on calcified tissues ISSN 2052-1219 (online)

Úvod: Mentálna anorexia (AN) je závažné psychosomatické ochorenie, ktoré sa rozvíja najmä v adolescentnom veku. Závažná nutričná deprivácia s rozvratom vnútorného prostredia a hormonálnou dysreguláciou ovplyvňujú aj hematopoézu kostný metabolizmus. Cieľom tejto práce bolo zistiť, či existuje vzájomný vzťah medzi poruchou hematopoézy zníženým kostným metabolizmom u dievčat mentálnou anorexiou. Metodika: Do štúdie zahrnutých 25 AN (priemerný vek 16,2...