Salivary oxidative stress markers represent a promising tool for monitoring of oral diseases. Saliva can often be contaminated by blood, especially in patients with periodontitis. The aim our study was to examine the impact blood contamination on measurement salivary markers. samples were collected from 10 healthy volunteers and artificially (final concentration 0.001–10%). Next, saliva 12 gingivitis control before after dental hygiene treatment. Markers measured all samples. Advanced...

The paper presents the application of natural materials, especially wood, which are relevant for human well-being in built environments health, social, and day care facilities. These properties were tested by a complex methodology case study wooden waiting room at National Oncology Institute Bratislava. In this space, experimental tests physiological responses further executed on 50 volunteers moving 20 min. article, EEG (electroencephalograph) (four persons) emotions from faces all our...

Oxidative stress markers are usually measured in plasma, a stable environment for biomarkers. Blood collection is invasive, but the use of alternative biofluids limited, due to high variability. In this study, we aimed establish reference values oxidative urine and saliva adult, healthy mice identify some sources Samples were obtained from 41 female 37 male CD-1 strain, aged 95-480 days, weighing 21-55 grams. Reference ranges TBARS (thiobarbituric acid reactive substances), AOPP (advanced...

The aim of this research was to compare the mental health families with children diagnosed autism spectrum disorder or typically developing children, during first and second wave COVID-19 outbreak in Slovakia. study is mainly focused on prevalence depression, anxiety, stress among parents maladaptive behavior sleep disturbances their children. Our sample consisted 332 caregivers, 155 which have disorder; 179 surveyed 153 wave. Extensive online parent questionnaire created, including...

The term "bacterial dysbiosis" is being used quite extensively in metagenomic studies, however, the identification of harmful bacteria often fails due to large overlap between bacterial species found healthy volunteers and patients. We hypothesized that pathogenic oral are individual-specific they correlate with oxidative stress markers saliva which reflect inflammatory processes cavity. Temporally direct lagged correlations taxa were computed individually for 26 who provided samples during...

The etiology of autism spectrum disorders (ASD) remains unknown, but associations between prenatal hormonal changes and ASD risk were found. consequences these on the steroidogenesis during a postnatal development are not yet well known. aim this study was to analyze steroid metabolic pathway in prepubertal neurotypical boys. Plasma samples collected from 62 boys 24 age sex-matched controls (CTRL). Eighty-two biomarkers detected using gas-chromatography tandem-mass spectrometry. We observed...

Abstract Effective regulation of emotions is essential in coping with everyday stressors. Although cognitive reappraisal and acceptance are both commonly employed to downregulate negative emotions, they differ many aspects. Their effectiveness real‐life situations can also be influenced by prior stress or a person's trait preferences for certain emotion (ER) strategies. In the present study, we compared ER strategies laboratory setting, while focussing on effect moderators. Ninety‐eight...

Plasma creatinine and urea are commonly used markers of kidney function in both acute chronic renal failure. The needed repeated blood collection is associated with pain, stress might lead to infections. Saliva has the potential be a non-invasive alternative diagnostic fluid. use saliva clinical practice limited, since many factors affect concentration salivary biomarkers. aim our study was analyze animal models disease. Bilateral nephrectomy adenine nephropathy were induced adult male mice....

Abstract Objectives The early diagnosis and monitoring of Crohn's disease (CD) orofacial granulomatosis (OFG) might be facilitated by assaying potential biomarkers in saliva. Markers oxidative stress inflammation were assayed salivas from patients with CD, OFG concurrent CD (OFG + CD). Subjects Unstimulated whole mouth saliva was collected 93 subjects, immunoglobulin A (IgA), lactoferrin (LF) myeloperoxidase (MPO) determined ELISA. antioxidant status spectrophotometrically. Results...

Erythrocytes are responsible for the transport of oxygen within organism, which is particularly important nerve tissues. Erythrocyte quality has been shown to be deteriorated in oxidative stress conditions. In this study, we measured same series markers plasma and erythrocytes compare differences between neurotypical children (controls) with autism spectrum disorder (ASD). We also focused on erythrocyte properties including their deformability, osmotic resistance, Na,K-ATPase activity,...

To evaluate the effect of food containing anthocyanin-rich wheat on oxidative status and behavior healthy rats.Twenty male rats were divided into control anthocyanin group. Oral glucose tolerance test was performed, proteinuria creatinine clearance measured. Behavioral analysis performed in Phenotyper cages. Serum tissues collected to measure markers stress antioxidant status.Anthocyanins significantly increased total capacity serum (P=0.039), decreased advanced oxidation protein products...

AimTo assess the impact of prenatal exposure to Maillard reaction products (MRPs) -rich diet and postnatal Coca-Cola consumption on metabolic status female rats. Diet rich in MRPs saccharose/fructose sweetened soft drinks is presumed impose increased risk development cardiometabolic afflictions, such as obesity or insulin resistance.MethodsAt first day pregnancy, 9 Wistar rats were randomized into two groups, pair-fed either with standard rat chow (MRP-) MRPs-rich (MRP+). Offspring from each...

Purpose of the study was to identify relationship among actual plasmatic levels steroid hormones and behavioral manifestations in boys with autism assess genetic contribution these manifestations.172 under 10 years age 135 neurotypical attended study. ADI-R ADOS-2 were used evaluate core symptom severities. Problem behavior assessed using BPI-01 questionnaire. Levels testosterone, estradiol, dehydroepiandrosterone, dehydroepiandrosterone-sulfate sex hormone binding globulin (SHBG) measured...

Thermally processed food contains advanced glycation end products (AGEs) including N-(carboxymethyl)lysine (CML). Higher AGEs or circulating CML were shown to be associated with pregnancy complications such as preeclampsia and gestational diabetes. It is unclear whether this association causal. The aim of our study was analyze the effects dietary CML-containing thermally on metabolism in pregnant rats. Animals fed standard AGE-rich diet from gestation day 1. Third group received via gavage....

The aetiology of inflammatory bowel diseases is unclear, but oxidative stress plays a key role in the pathogenesis. Anthocyanins--plant polyphenols--were shown to have antioxidant and anti-inflammatory properties. aim this study was investigate potential protective effects anthocyanins on status mice with chemically induced colitis. Adult male were randomly divided into control group drinking tap water colitis 1% dextran sulphate sodium solution. Animals had ad libitum access wheat-based...

Schizophrenia is associated with numerous abnormalities, including imbalances in all hormonal axes, among which steroids play a major role. Steroidomic studies therefore represent promising tool for early diagnosis and appropriate treatment of schizophrenia. A total 51 adult male schizophrenics aged 27 (22, 34) years (shown as median quartiles) 16 healthy controls (HCs) 28 (25, 32) were enrolled into this study. Our results showed the effective differentiation men schizophrenia from based on...

The diagnostic prevalence of autism spectrum disorders (ASD) shows boys to be more affected than girls. Due this reason, there is a lack research including and observing ASD Present study was aimed detect hormones steroidogenesis pathway in prepubertal girls (n = 16) diagnosed with sex age matched neurotypical controls (CTRL, n 16). Collected plasma served for detection conjugated unconjugated steroids using gas chromatography tandem-mass spectrometry. We observed higher levels modulating...

The second to fourth digit ratio (2D:4D) is according previous studies a likely biomarker for prenatal testosterone exposure and its effect on the human brain. It was found be linked autism spectrum disorders (ASD). Recently, 2D:4D raised lot of questions with regard stability autism-related behaviors. Here, we present cross-sectional study in boys (N = 91, mean age 7.63) adults 36 22.8) ASD as well neurotypical students, 506 participants total. Digit assessed by taking measurements from...

Many studies use stimulated saliva for the assessment of cortisol. However, it is not yet clear how stimulation affects flow specific markers. The aim was to assess whether salivation physiological cortisol during a stressing day as compared an ordinary day. second show normalising factor outcome study.Stimulated taken from 42 children at 8:00 a.m. and 12:00 on two separate days one month apart. During first day, were exposed stress situation, while considered control concentration analysed...

In an attempt to develop potential and selective anti-proliferative agents, a series of novel benzothiazine-piperazine derivatives 8a–i 10a–g were synthesized by coupling 2H-1,4-benzothiazin-3(4H)-one with various amines 7a–i 9a–g in excellent yields evaluated for their vitro activity against four cancer cell lines, HeLa (cervical), MIAPACA (pancreatic), MDA-MB-231 (breast) IMR32 (neuroblastoma). inhibitory indicated that compounds 8a, 8d, 8g, 10a, 10b, 10e, 10f found be good agents. Among...

The study focuses on current issues of adaptive behavior in individuals with autism spectrum disorder (ASD) and the possible risk factor environmental tobacco smoke (ETS). Children examined at Academic Research Center for Autism (ARCA) Bratislava were involved study. sample included 84 children (71 boys) ASD (average age 5.35 years) a non-ASD group 24 (20 boys; average 8.10 years). "ETS Questionnaire" focused detection parental smoking habits other ETS exposures. concentrations cotinine...