A5057464620- Prenatal Screening and Diagnostics
- Extracellular vesicles in disease
- Autism Spectrum Disorder Research
- MicroRNA in disease regulation
- Fetal and Pediatric Neurological Disorders
- Genetics and Neurodevelopmental Disorders
- Parvovirus B19 Infection Studies
- Child Development and Digital Technology
- Molecular Biology Techniques and Applications
- Reproductive System and Pregnancy
- Gut microbiota and health
- Pregnancy and preeclampsia studies
- Genomic variations and chromosomal abnormalities
- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
- Child Nutrition and Feeding Issues
- Clostridium difficile and Clostridium perfringens research
- Genetically Modified Organisms Research
- Circular RNAs in diseases
- Bone Tissue Engineering Materials
- Stress Responses and Cortisol
- Attention Deficit Hyperactivity Disorder
- Cancer-related molecular mechanisms research
- Advanced biosensing and bioanalysis techniques
- Bone Metabolism and Diseases
Comenius University Bratislava
2015-2025
Czech Academy of Sciences, Institute of Physiology
2017-2024
Center for Autism and Related Disorders
2024
The University of Texas MD Anderson Cancer Center
2018
Institute of Molecular Biology of the Slovak Academy of Sciences
2018
Slovak Medical University
2018
Slovak Academy of Sciences
2018
Vysoká Škola Zdravotníctva a Sociálnej Práce sv. Alžbety
2018
Northwestern University
2018
Princess Margaret Cancer Centre
2018
Specific applications and modern technologies, like non-invasive prenatal testing, cancer diagnostic next generation sequencing, are currently in the focus of researchers worldwide. These have common characteristics use highly fragmented DNA molecules for analysis. Hence, performance molecular methods, concentration is a crucial parameter; we compared influence different levels fragmentation on accuracy measurements.In our comparison, most commonly used methods measurement...
Abstract intestinal microbiota is becoming a significant marker that reflects differences between health and disease status also in terms of gut-brain axis communication. Studies show children with autism spectrum disorder (ASD) often have mix gut microbes distinct from the neurotypical children. Various assays are being used for investigation were considered to be universal. However, newer studies showed protocol preparing DNA sequencing libraries key factor influencing results...
Objectives The aims of this study were to test the utility benchtop NGS platforms for NIPT trisomy 21 using previously published z score calculation methods and optimize sample preparation data analysis with use in silico physical size selection methods. Methods Samples from 130 pregnant women analyzed by whole genome sequencing on systems Ion Torrent PGM MiSeq. targeted yield 3 million raw reads each platform was used calculation. impact analytical performance studied. Results Using a value...
Extracellular DNA (ecDNA) is outside of cells, which a result various mechanisms. EcDNA believed to be cause pathogeneses as well their potential biomarker. also part small extracellular vesicles (sEVs) from cell cultures. If ecDNA present in sEVs plasma, membrane may protect it degradation by deoxyribonucleases. Moreover, play role the intercellular communication, and they can therefore transfer between cells. The aim this study was investigate presence isolated fresh human plasma...
Abstract Background: The cell free fetal DNA (cffDNA) present in plasma of pregnant women represents an important alternative source for non-invasive prenatal diagnosis. Due to the low quantity and increased fragmentation cffDNA, choice extraction method is a crucial step downstream analyses. Methods: In our study, three spin column-based kits isolation cffDNA [DNA Blood Mini Kit (DBM), DSP Virus (DSP) Circulating Nucleic Acid (CNA) Kit] were compared. Original optimized protocol used...
The etiology of autism spectrum disorders (ASD) remains unknown, but associations between prenatal hormonal changes and ASD risk were found. consequences these on the steroidogenesis during a postnatal development are not yet well known. aim this study was to analyze steroid metabolic pathway in prepubertal neurotypical boys. Plasma samples collected from 62 boys 24 age sex-matched controls (CTRL). Eighty-two biomarkers detected using gas-chromatography tandem-mass spectrometry. We observed...
Autism spectrum disorders (ASD) are neurodevelopmental characterized by impaired social interaction and communication, as well repetitive behavior restricted interests. There is convincing evidence that the intestinal inflammation involved in etiology of ASD. Increased levels inflammatory markers were shown to be associated with more aberrant behaviors communication subjects Calprotectin feces produced activated neutrophils epithelial cells gut mucosa, its reflect local gastrointestinal...
Autism spectrum disorder is a heterogeneous neurodevelopmental disease. Currently, no biomarker of this disease known. Diagnosis performed through observation, standardized behavioral scales, and interviews with parents. In practice, diagnosis often delayed to the average age four years or even more which adversely affects child’s perspective. A laboratory method allowing detect at earlier stages great need, as could help patients start treatment younger age, prior clinical diagnosis. Recent...
Circulating tumor cells (CTCs) play a pivotal role in dissemination and progression, are considered to be critical part of the metastatic cascade. The aim present research article was examine breast cancer‑specific mutations primary cancer (PBC) using targeted resequencing. A total 78 patients with PBC were enrolled into this translational study. Reverse transcription‑quantitative PCR assay for expression epithelial markers (CK19) or epithelial‑to‑mesenchymal transition (EMT)‑related genes...
Purpose of the study was to identify relationship among actual plasmatic levels steroid hormones and behavioral manifestations in boys with autism assess genetic contribution these manifestations.172 under 10 years age 135 neurotypical attended study. ADI-R ADOS-2 were used evaluate core symptom severities. Problem behavior assessed using BPI-01 questionnaire. Levels testosterone, estradiol, dehydroepiandrosterone, dehydroepiandrosterone-sulfate sex hormone binding globulin (SHBG) measured...
Background: Circulating nucleic acids acquired non-invasively have been confirmed as useful biomarkers in cancer and prenatal medicine. The most important molecules the field of circulating research are DNA miRNA. In this study, possibility co-isolation total DNA, cell-free fetal miRNA from plasma pregnant women was tested, yields co-isolated using two commercial kits three protocols were compared.
In this article, we present a case study of five-year-old girl with autism and developmental delay, conducted at the Academic Center for Autism Research in Bratislava, Slovakia. The was diagnosed using Diagnostic Observation Schedule-Second Edition (ADOS-2) Interview-Revised (ADI-R) instruments met criteria spectrum disorder. Intellectual functioning markedly below-average range, as indicated by Snijders-Oomen Nonverbal Intelligence Test-Revised (SON-R) examination, her level adaptive...