A5049950685- Preterm Birth and Chorioamnionitis
- Pregnancy-related medical research
- Neonatal Respiratory Health Research
- Pregnancy and preeclampsia studies
- Reproductive tract infections research
- Reproductive System and Pregnancy
- Neonatal and Maternal Infections
- Assisted Reproductive Technology and Twin Pregnancy
- Maternal and Perinatal Health Interventions
- Congenital Anomalies and Fetal Surgery
- Urological Disorders and Treatments
- Abdominal Trauma and Injuries
- Prenatal Screening and Diagnostics
- Pelvic floor disorders treatments
- Infant Nutrition and Health
- Intestinal Malrotation and Obstruction Disorders
- Medieval European History and Architecture
- Historical and Archaeological Studies
- Biomarkers in Disease Mechanisms
- Infant Development and Preterm Care
- Pregnancy and Medication Impact
- Cervical Cancer and HPV Research
- Congenital Diaphragmatic Hernia Studies
- Pelvic and Acetabular Injuries
- Oral microbiology and periodontitis research
University Hospital Hradec Králové
2015-2024
Krajská Zdravotní
2023-2024
Charles University
2014-2024
National Institutes of Health
2020
Eunice Kennedy Shriver Center
2020
Wayne State University
2020
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2020
University of Pardubice
2012-2014
University of Hradec Králové
2013
Ocní klinika
2006-2011
To characterize subgroups of preterm prelabor rupture membranes (PPROM) and short-term neonatal outcomes based on the presence absence intraamniotic inflammation (IAI) and/or microbial invasion amniotic cavity (MIAC).One hundred sixty-six Caucasian women with singleton pregnancies were included in this study. Amniotic fluid samples obtained by transabdominal amniocentesis (n=166) assayed for interleukin-6 levels a lateral flow immunoassay. The Ureaplasma species, Mycoplasma hominis,...
Marian Kacerovsky*, Ivana Musilova, Ali Khatibib, Kristin Skogstrandd, David M. Hougaardd, Vojtech Tambore, Jindrich Tosnera & Bo Jacobssona Department of Obstetrics and Gynecology, Faculty Medicine Hradec Kralove, Charles University in Prague, Hospital Czech Republicb Sahlgrenska Hospital, Gothenburg, Swedenc Pardubice, Republicd Clinical Biochemistry Immunology, Statens Serum Institut, Copenhagen, Denmarke Biomedical Research Center, Republicf Institute Public Health, Oslo, Norway
To evaluate Ureaplasma species and Mycoplasma hominis DNA in the cervical fluid their association with microbial invasion of amniotic cavity (MIAC) and/or histological chorioamnionitis (HCA) pregnancies complicated by preterm prelabor rupture membranes (PPROM).A prospective study 68 women singleton PPROM between 24(0/7) 36(6/7) weeks was conducted. Cervical were collected from all at time admission. The identified using specific real-time PCR.Ureaplasma 59% (40/69) samples. Women presence...
Objective: To determine the impact of histological chorioamnionitis (HCA) and funisitis on neonatal outcome in preterm prelabor rupture membranes (PPROM) pregnancies.Methods: Women with PPROM between 24 + 0 to 36 6 weeks gestation, admitted Department Obstetrics Gynecology at University Hospital Hradec Kralove Czech Republic, July 2008 October 2010, were enrolled study (n = 231).Results: The incidence early-onset sepsis (EOS) differed significantly neonates born women without HCA, after...
Objective To analyze the cervical microbiota in women with preterm prelabor rupture of membranes (PPROM) by pyrosequencing and to document associations between microbiota, inflammatory response, microbial invasion amniotic cavity (MIAC), histological chorioamnionitis, intraamniotic infection (IAI). Study Design Sixty-one singleton pregnancies complicated PPROM were included study. Specimens fluid collected on admission. The was assessed 16S rRNA gene sequencing pyrosequencing. Interleukin...
Objective: To evaluate the influence of microbial invasion amniotic cavity (MIAC) and histological chorioamnionitis (HCA) on intensity fetal inflammatory response occurrence syndrome (FIRS) in preterm prelabor rupture membranes (PPROM).
Objective To evaluate maternal serum C-reactive protein (CRP) concentrations in pregnancies complicated by preterm prelabor rupture of membranes (PPROM) relation to the presence microbial invasion amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI). Methods Two hundred and eighty-seven women with singleton PPROM between 2014 2016 were included this study. Maternal blood fluid samples collected at time admission. CRP concentration was measured using a high-sensitivity...
The placenta plays a crucial role beyond nutrient transfer, acting as dynamic endocrine organ that significantly influences maternal physiology and fetal development. It responds rapidly to even slight changes in the utero environment promote survival. Disruptions placental function are increasingly recognized key contributors origins of neurodevelopmental disorders. In this study, we employed advanced technology induce intrauterine inflammation through ultrasound-guided administration LPS...
Objective This study evaluated maternal C-reactive protein (CRP) as a predictor of microbial invasion the amniotic cavity (MIAC) and histological chorioamnionitis (HCA) in women with preterm prelabor rupture membranes (PPROM) before after 32 weeks gestation. Methods was prospective observational cohort 386 women. Maternal serum CRP concentrations were evaluated, fluid samples obtained via transabdominal amniocentesis at time admission. Placentas underwent histopathological examination...
Objective: To determine the cervical fluid interleukin (IL)-6 and IL-8 levels in pregnancies complicated by preterm prelabor rupture of membranes (PPROM) association these interleukins with microbial invasion amniotic cavity (MIAC) histological chorioamnionitis (HCA).Methods: Sixty women singleton were included this study. Cervical was sampled at time admission using Dacron polyester swabs, which placed into endocervical canal for 20 s. IL-6 determined ELISA. The management PPROM active...
Introduction: We aimed to compare the amniotic fluid interleukin (IL)-6 concentrations measured using automated electrochemiluminescence immunoassay method and ELISA, establish an IL-6 concentration cut-off value for intra-amniotic inflammation (IAI) in preterm prelabor rupture of membranes (PPROM), which can be used method.Materials methods: A total 120 women with PPROM were included this study. Amniotic samples obtained through transabdominal amniocentesis. assessed both current gold...
The aim of this study was to identify the rates 2 phenotypes intra-amniotic inflammation: infection (with microbial invasion amniotic cavity [MIAC]) and sterile inflammation (without MIAC), their outcomes, among women with cervical insufficiency prolapsed fetal membranes.This is a retrospective admitted Department Obstetrics Gynecology, University Hospital Hradec Kralove between January 2014 May 2020. Transabdominal amniocentesis evaluate (amniotic fluid interleukin-6) MIAC (culturing...
Objective To evaluate umbilical cord interleukin (IL)-6 and funisitis as independent predictors of early-onset neonatal sepsis (EONS) in preterm prelabor rupture membranes (PPROM). Design Prospective cohort study. Setting Evaluation IL-6 PPROM. Population 176 women with PPROM between 23+0−36+6 weeks gestation. Methods Umbilical was assayed by ELISA. Funisitis defined according to the Salafia classification. Data adjusted gestational age at delivery prenatal administration corticosteroids...
Background Preterm prelabor rupture of membranes (PPROM) complicated by microbial invasion the amniotic cavity (MIAC) leading to histological chorioamnionitis (HCA) significantly impacts perinatal morbidity. Unfortunately, no well-established tool for identifying PPROM patients threatened these disorders is available. Methodology/Principal Findings We performed an unbiased exploratory analysis fluid proteome changes due MIAC and HCA. From among top five proteins that showed most profound...
The main aim of this study was to determine the relationship between maternal white blood cell (WBC) count at time hospital admission in pregnancies complicated by preterm prelabor rupture membranes (PPROM) and presence microbial invasion amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI). second test WBC diagnostic indices with respect MIAC IAI.Four hundred seventy-nine women singleton PPROM, February 2012 June 2017, were included study. Maternal fluid samples collected...
Purpose To determine whether the measurement of transverse diameter fetal thymus is value in identification either histologic chorioamnionitis or funisitis pregnancies complicated by preterm prelabor rupture membranes (PPROM). Methods The was measured 216 fetuses from PPROM pregnancies. A small defined as a thymic below fifth percentile according to previously published nomogram. placenta, membranes, and umbilical cord were assessed for presence inflammation. Results identified 69% (150/216)...
To determine whether decreased fetal growth velocity precedes antepartum death and to evaluate is a better predictor of compared single biometric measurement at the last available ultrasound scan prior diagnosis demise.This was retrospective, longitudinal study 4285 singleton pregnancies in African-American women who underwent least two examinations between 14 32 weeks gestation delivered liveborn neonate (controls; n = 4262) or experienced (cases; 23). Fetal defined as diagnosed ≥ 20...