A5004322396- Extracellular vesicles in disease
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Cell Adhesion Molecules Research
- Escherichia coli research studies
- RNA and protein synthesis mechanisms
- Galectins and Cancer Biology
- Immune cells in cancer
- Cardiac Fibrosis and Remodeling
- Circular RNAs in diseases
- Complement system in diseases
- Cancer-related molecular mechanisms research
- Glycosylation and Glycoproteins Research
- Viral Infections and Vectors
- Nanoplatforms for cancer theranostics
- Viral Infectious Diseases and Gene Expression in Insects
- Phagocytosis and Immune Regulation
University of Gothenburg
2014-2024
AstraZeneca (Sweden)
2024
The isolation of extracellular vesicles (EVs) from blood is great importance to understand the biological role circulating EVs and develop as biomarkers disease. Due concurrent presence lipoprotein particles, however, one most difficult body fluids isolate from. aim this study was a robust method characterise with minimal contamination by plasma proteins particles. Plasma serum were collected healthy subjects, isolated size-exclusion chromatography (SEC), particles being present in fractions...
Background Extracellular vesicles (EV), the collective term for released from cells, consist of vesicle species ranging in size 30 nm to 5 µm diameter. These are most commonly isolated by differential centrifugations, which pellets particles based on their movement through liquid medium they immersed. Multiple parameters, including utilization different rotor types, can influence yield and purity vesicles; however, understanding how these factors affect is limited. Materials methods Here, we...
Extracellular vesicles (EVs) are produced by all known organisms and important for cell communication physiology. Great morphological diversity has been described regarding EVs found in body fluids such as blood plasma, breast milk, ejaculate. However, a detailed analysis never performed on exosomes when purified from single type. In this study we analysed quantified, via multiple electron microscopy techniques, the morphology of human mast line HMC-1. The results revealed wide exosome...
The majority of extracellular vesicle (EV) studies conducted to date have been performed on cell lines with little knowledge how well these represent the characteristics EVs in vivo. aim this study was establish a method isolate and categorize subpopulations isolated directly from tumour tissue. First we established an isolation protocol for metastatic melanoma tissue, which included enzymatic treatment (collagenase D DNase). Small large were differential ultracentrifugation, further...
Extracellular vesicles have the capacity to transfer lipids, proteins, and nucleic acids between cells, thereby influencing recipient cell's phenotype. While role of RNAs in EVs has been extensively studied, function DNA remains elusive. Here, we distinguished novel heterogeneous subpopulations small extracellular (sEVs) based on their content topology. Low- high-density sEV subsets from a human mast cell line (HMC-1) an erythroleukemic (TF-1) were separated using high-resolution iodixanol...
Abstract Extracellular vesicles (EVs) are important mediators of intercellular communication that change the recipient cell by shuttling lipids, RNA, or protein cargo between cells. Here, we investigate topology found in EVs, as this can fundamentally influence biological effects EVs. A multiple proteomics approach, combining proteinase treatment and biotin tagging, shows many proteins cytosolic origin localized on surface detailed analysis EV proteome at peptide level revealed a number...
Extracellular vesicles (EVs), including exosomes and microvesicles, are secreted from all cells, convey messages between cells in health disease. However, the diversity of EV subpopulations is only beginning to be explored. Since EVs have been implicated tumour microenvironmental communication, we started determine specifically this tissue. To do this, isolated directly patient melanoma metastatic tissues. Using membrane isolation mass spectrometry analysis, discovered enrichment...
Cholera toxin (CT) enters and intoxicates host cells after binding cell surface receptors via its B subunit (CTB). We have recently shown that in addition to the previously described partner ganglioside GM1, CTB binds fucosylated proteins. Using flow cytometric analysis of primary human jejunal epithelial granulocytes, we now show correlates with expression Lewis X (LeX) glycan. This is competitively blocked by oligosaccharides fucose-binding lectins. LeX glycan vitro when this moiety linked...
Abstract Heart failure remains a leading cause of mortality. Therapeutic intervention for heart would benefit from targeted delivery to the damaged tissue. Here, we applied in vivo peptide phage display coupled with high-throughput Next-Generation Sequencing (NGS) and identified peptides specifically targeting cardiac We established bioinformatics pipeline identification peptides. Hit demonstrated preferential uptake by human induced pluripotent stem cell (iPSC)-derived cardiomyocytes...
Abstract Background Asthma is a common and heterogeneous disease that includes subgroups characterized by type 2 (T2) or 17 (T17) immune responses for which there need to identify the underlying mechanisms biomarkers in order develop specific therapies. These can be defined airway epithelium gene signatures has also been implicated play significant role asthma pathology. Extracellular vesicles (EVs) carry functional biomolecules participate cell-to-cell communication both health disease,...
Abstract Colon cancer is one of the most commonly occurring tumours among both women and men, over past decades incidence has been on rise. As such, need for biomarker identification as well an understanding underlying disease mechanism never greater. Extracellular vesicles are integral mediators cell‐to‐cell communication offer a unique opportunity to study machinery that drives progression, they also function vectors potential biomarkers. Tumour tissue healthy mucosal from colons ten...
Abstract Extracellular vesicles (EVs), including exosomes and microvesicles, are secreted from all cells, convey messages between cells in health disease. However, the diversity of EV subpopulations only beginning to be explored. Since EVs have been implicated tumor microenvironmental communication, we started determine specifically this tissue. To do this, isolated directly patient melanoma metastatic tissues. Using membrane isolation mass spectrometry analysis, discovered enrichment...
Abstract Extracellular vesicles (EVs) are produced by all known organisms and have important roles in cell communication physiology. Exosomes the literature to be small round EVs (40 100 nm diameter) commonly purified with a serial ultracentrifugation protocol followed density gradient floatation. Great morphological diversity has been described before regarding found body fluids such as blood plasma, breast milk ejaculate. However, detailed analysis never performed on exosomes from single...