A5004860286- Mesenchymal stem cell research
- Extracellular vesicles in disease
- Neonatal Respiratory Health Research
- Tissue Engineering and Regenerative Medicine
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- MicroRNA in disease regulation
- Occupational and environmental lung diseases
- Pulmonary Hypertension Research and Treatments
- RNA Interference and Gene Delivery
- Bacterial Infections and Vaccines
- Advanced Nanomaterials in Catalysis
- Hydrogen's biological and therapeutic effects
- Sarcoidosis and Beryllium Toxicity Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Congenital Diaphragmatic Hernia Studies
- Respiratory Support and Mechanisms
- Intensive Care Unit Cognitive Disorders
- Amoebic Infections and Treatments
- Medical Imaging and Pathology Studies
- Immune Response and Inflammation
- Inhalation and Respiratory Drug Delivery
- Pleural and Pulmonary Diseases
- Cerebrospinal fluid and hydrocephalus
University of Gothenburg
2017-2023
Karolinska Institutet
2020
Johns Hopkins University
2016-2020
Universidade Federal do Rio de Janeiro
2011-2018
Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
2016-2018
Hospital de Clínicas de Porto Alegre
2017
Universidade Federal do Rio Grande do Sul
2017
Johns Hopkins Medicine
2016
Czech Academy of Sciences, Institute of Biophysics
2016
We sought to assess whether the effects of mesenchymal stromal cells (MSC) on lung inflammation and remodeling in experimental emphysema would differ according MSC source administration route. Emphysema was induced C57BL/6 mice by intratracheal (IT) porcine pancreatic elastase (0.1 UI) weekly for 1 month. After last instillation, saline or MSCs (1-105), isolated from either mouse bone marrow (BM), adipose tissue (AD) (L), were administered intravenously (IV) IT. week, euthanized. Regardless...
Sepsis remains a source of high mortality in hospitalized patients despite proper antibiotic approaches. Encouragingly, mesenchymal stromal cells (MSCs) and their produced extracellular vesicles (EVs) have been shown to elicit anti-inflammatory effects multiple inflammatory conditions including sepsis. However, EVs are generally released from mammalian relatively low amounts, high-yield isolation is still challenging due complicated procedure. To get over these limitations, very similar can...
Abstract Mesenchymal stromal cells (MSCs) from different sources have differential effects on lung injury. To compare the of murine MSCs bone marrow (BM), adipose tissue (AD), and (LUNG) inflammatory remodeling processes in experimental allergic asthma, female C57BL/6 mice were sensitized challenged with ovalbumin (OVA) or saline (C). Twenty-four hours after last challenge, received either (50 µl, SAL), BM-MSCs, AD-MSCs, LUNG-MSCs (105 per mouse 50 µl total volume) intratracheally. At 1...
Mesenchymal stem cells-based therapies have shown promising effects in experimental acute respiratory distress syndrome. Different mesenchymal cells sources may result diverse diseases; however, there is no information regarding the best source of to treat pulmonary We tested hypothesis that derived from bone marrow, adipose tissue, and lung tissue would lead different beneficial on distal organ damage syndrome.Animal study primary cell culture.Laboratory investigation.Seventy-five Wistar...
Silicosis is an occupational disease that affects workers who inhale silica particles, leading to extensive lung fibrosis and ultimately causing respiratory failure. Mesenchymal stromal cells (MSCs) have been shown exert therapeutic effects in diseases represent alternative treatment for silicosis. Recently, it has suggested similar can be achieved by the use of extracellular vesicles (EVs) obtained from MSCs. The aim this study was investigate adipose-tissue-derived MSCs (AD-MSCs) or their...
Sepsis induced cardiac dysfunction (SIC) is a severe complication to sepsis which significantly worsens patient outcomes. It known that bacteria have the capacity release outer membrane vesicles (OMVs), are nano-sized bilayered composed of lipids and proteins, can induce fatal inflammatory response. The aim this study was determine whether OMVs from uropathogenic Escherichia coli strain dysfunction, elucidate any mechanisms involved. irregular Ca2+ oscillations with decreased frequency in...
We compared the effects of bone marrow-derived mononuclear cells (BMMCs) and mesenchymal stromal (MSCs) on airway inflammation remodeling lung mechanics in experimental allergic asthma. C57BL/6 mice were sensitized challenged with ovalbumin (OVA group). A control group received saline using same protocol. Twenty-four hours after last challenge, groups further randomized into subgroups to receive saline, BMMCs (2×10(6)) or MSCs (1×10(5)) intratracheally. BMMC MSC administration decreased cell...
Abstract Pulmonary sarcoidosis has unknown etiology, a difficult diagnostic procedure and no curative treatment. Extracellular vesicles including exosomes are nano-sized entities released from all cell types. Previous studies of bronchoalveolar lavage fluid (BALF) patients have revealed pro-inflammatory components abilities, but sources mechanisms not been identified. In the current study, we found that BALF patients, healthy individuals, induced dose-dependent elevation intracellular IL-1β...
Allergic asthma is associated with airflow obstruction and hyper-responsiveness that arises from airway inflammation remodeling. Cell therapy mesenchymal stem cells (MSC) has been shown to attenuate in models, similar effects have recently observed using extracellular vesicles (EV) obtained these cells. Biologically functional can also be artificially generated MSC by extruding through membranes produce EV-mimetic nanovesicles (NV). In this study, we aimed determine the of different...
Asthma is characterized by a chronic inflammatory process which may lead to several changes in bone marrow cell composition. We hypothesized that mononuclear cells (BMMCs) obtained from ovalbumin (OVA)-induced lung inflammation mice promote different effects compared BMMCs healthy donors model of allergic asthma.C57BL/6 were randomly assigned two groups. In the OVA group, sensitized and challenged with ovalbumin, while animals (control group) received saline using same protocol. analyzed...
Administration of bone marrow mononuclear cells (BMMCs) modulates lung inflammation and fibrosis in experimental silicosis. However, no studies have evaluated whether silicosis affects the efficacy autologous BMMCs treatment. We hypothesized that obtained from healthy or silicotic mice may improve function, but they might affect inflammatory fibrotic processes differently C57BL/6 were randomly divided into control (C) (SIL) groups. Mice SIL group instilled with silica particles...
Development of improved drug and gene delivery systems directly into the lungs is highly desirable given important burden respiratory diseases. We aimed to evaluate safety efficacy liposomes composed photopolymerized lipids (1,2-bis-(tricosa-10,12-diynoyl)-sn-glycero-3-phosphocholine) associated with amino acids as vectors for healthy animals. Lipopolymer vesicles, in particular, are more stable than other types liposomes. In this study, lipopolymers were L-arginine, L-tryptophan, or...
<b>Background:</b> Silicosis is a chronic lung disease and with no effective therapy. <b>Aim:</b> To investigate the effects of bone-marrow mononuclear cells (BMMCs) obtained from healthy silicotic donors on morpho-function in experimental silicosis. <b>Methods:</b> C57BL/6 mice were randomly divided control (C) silicosis (SIL) groups, intratracheally instilled saline (50mL) or silica (20mg/50mL), respectively, then treated intravenously (Sal) BMMCs (2x10<sup>6</sup>) (ht) (sc) mice....