A5076824677- Respiratory Support and Mechanisms
- Mesenchymal stem cell research
- Neonatal Respiratory Health Research
- Tissue Engineering and Regenerative Medicine
- Neuroinflammation and Neurodegeneration Mechanisms
- Inflammation biomarkers and pathways
- Cardiac Arrest and Resuscitation
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Long-Term Effects of COVID-19
- Intensive Care Unit Cognitive Disorders
- Hydrogen's biological and therapeutic effects
- Malaria Research and Control
- Sepsis Diagnosis and Treatment
- Neonatal and fetal brain pathology
- RNA Interference and Gene Delivery
- Mechanical Circulatory Support Devices
- Neurogenesis and neuroplasticity mechanisms
- Inhalation and Respiratory Drug Delivery
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Pleural and Pulmonary Diseases
- SARS-CoV-2 and COVID-19 Research
- Innovation and Socioeconomic Development
- Vagus Nerve Stimulation Research
- Nosocomial Infections in ICU
- Phagocytosis and Immune Regulation
Fundação Oswaldo Cruz
2013-2025
Universidade Federal do Rio de Janeiro
2008-2021
Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
2010-2021
International Olive Council
2021
St. Michael's Hospital
2013-2020
Unity Health Toronto
2020
Ottawa Hospital
2016
University of Ottawa
2016
University of Toronto
2013-2014
University of Minnesota
2010
Although mesenchymal stromal (stem) cell (MSC) administration attenuates sepsis-induced lung injury in pre-clinical models, the mechanism(s) of action and host immune system contributions to its therapeutic effects remain elusive. We show that treatment with MSCs decreased expression host-derived microRNA (miR)-193b-5p increased target gene, tight junctional protein occludin (Ocln), lungs from septic mice. Mutating Ocln 3' untranslated region miR-193b-5p binding sequence impaired mRNA....
Oxidative stress is considered one of the early underlying contributors acute lung injury (ALI) and ventilator-induced (VILI). DJ-1, also known as PARK7, has a well-established role an antioxidant. We have previously shown maintaining oxidative balance via ATF3-Nrf2 axis was important in protection from ALI. Here, we exclusively characterize DJ-1 sterile LPS-induced ALI VILI. protein expression increased after LPS treatment human epithelial endothelial cell lines lungs wild-type mice....
Recent evidence suggests that mesenchymal stem cells may attenuate lung inflammation and fibrosis in acute injury. However, so far, no study has investigated the effects of cell therapy on time course structural, mechanical, remodeling properties pulmonary or extrapulmonary injury.Prospective randomized controlled experimental study.University research laboratory.One hundred forty-three females 24 male C57BL/6 mice.Control mice received saline solution intratracheally (0.05 mL, control)...
Effective and rapid bacterial clearance is a fundamental determinant of outcomes in sepsis. DJ-1 well-established reactive oxygen species (ROS) scavenger.Because cellular ROS status pivotal to inflammation killing, we determined the role sepsis.We used cell murine models with gain- loss-of-function experiments, plasma, cells from patients sepsis.Stimulation bone marrow-derived macrophages (BMMs) endotoxin resulted increased mRNA protein expression. Cellular mitochondrial was DJ-1-deficient...
Parkinson's disease (PD) is a progressive degenerative of the central nervous system associated with neuroinflammation and microglial cell activation. Chemokine signaling regulates neuron-glia communication triggers inflammatory profile. Herein, we identified neuronal chemokine CCL21 as major cause imbalance through CCR7 receptor pathway therapeutic implications for PD. In humans, found that transcript expression was increased in dopaminergic neurons (DANs) substantia nigra PD patients....
To hypothesize that bone marrow-derived mononuclear cell (BMDMC) therapy might act differently on lung and distal organs in models of pulmonary or extrapulmonary acute injury with similar mechanical compromises. The pathophysiology differs according to the type primary insult.Prospective, randomized, controlled, experimental study.University research laboratory.In control animals, sterile saline solution was intratracheally (0.05 mL) intraperitoneally (0.5 injected. Acute animals received...
Objective: To investigate the effects of rate airway pressure increase and duration recruitment maneuvers on lung function activation inflammation, fibrogenesis, apoptosis in experimental acute injury. Design: Prospective, randomized, controlled study. Setting: University research laboratory. Subjects: Thirty-five Wistar rats submitted to injury induced by cecal ligation puncture. Interventions: After 48 hrs, animals were randomly distributed into five groups (seven each): 1) nonrecruited...
We hypothesized that bone marrow-derived mononuclear cell (BMDMC) therapy protects the lung and consequently heart in experimental elastase-induced emphysema. Twenty-four female C57BL/6 mice were intratracheally instilled with saline (C group) or porcine pancreatic elastase (E once a week during 4 weeks. C E groups randomized into subgroups receiving (SAL) male BMDMCs (2 × 10(6), CELL) intravenously 3h after first instillation. Compared to E-SAL group, E-CELL showed, at 5 weeks: lower mean...
Survivors of sepsis are frequently left with significant cognitive and behavioral impairments. These complications derive from nonresolving inflammation that persists following hospital discharge. To date, no study has investigated the effects mesenchymal stromal cell therapy on blood-brain barrier, astrocyte activation, neuroinflammation, alterations in experimental sepsis.
Introduction: Sepsis is defined as a multifactorial debilitating condition with high risks of death. The intense inflammatory response causes deleterious effects on the brain, called sepsis-associated encephalopathy. Neuroinflammation or pathogen recognition are able to stress cells, resulting in ATP (Adenosine Triphosphate) release and P2X7 receptor activation, which abundantly expressed brain. contributes chronic neurodegenerative neuroinflammatory diseases; however, its function long-term...
This study tests the hypothesis that bone marrow-derived mononuclear cell (BMDMC) therapy may reduce lung inflammation and fibrosis leading to an improvement in respiratory mechanics a murine model of silicosis. 52 female C57BL/6 mice were randomly assigned into four groups. In silica group (SIL), suspension (20 mg/50 μL saline) was intratracheally instilled. control animals, 50 saline administered intratracheally. At 1 h, SIL groups further randomised, receiving BMDMC (2×10 6 i.v....
Recruitment maneuvers (RMs) seem to be more effective in extrapulmonary acute lung injury (ALI), caused mainly by sepsis, than pulmonary ALI. Nevertheless, the maintenance of adequate volemic status is particularly challenging sepsis. Since interaction between and RMs not well established, we investigated effects on distal organs presence hypovolemia, normovolemia, hypervolemia a model induced ALI was cecal ligation puncture surgery 66 Wistar rats. After 48 h, animals were anesthetized,...
In acute lung injury, recruitment maneuvers have been used to open collapsed lungs and set positive end-expiratory pressure, but their effectiveness may depend on the degree of injury. This study uses a single experimental model with different degrees injury tests hypothesis that beneficial or deleterious effects depending severity We speculated worsen mechanical stress in presence alveolar edema.Prospective, randomized, controlled study.University research laboratory.Thirty-six Wistar rats...
Ventilator-induced lung injury (VILI) contributes to mortality in patients with acute respiratory distress syndrome, the most severe form of (ALI). Absence activating transcription factor 3 (ATF3) confers susceptibility ALI/VILI. To identify cell-specific ATF3-dependent mechanisms ALI/VILI, we generated ATF3 chimera by adoptive bone marrow (BM) transfer and randomized inhaled saline or lipopolysacharide (LPS) presence mechanical ventilation (MV). Adenovirus vectors silence overexpress were...
We hypothesized that bone marrow-derived mononuclear cells (BMDMC) would attenuate the remodeling process in a chronic allergic inflammation model. C57BL/6 mice were assigned to two groups. In OVA, sensitized and repeatedly challenged with ovalbumin. Control (C) received saline under same protocol. C OVA further randomized receive BMDMC (2 × 106) or intravenously 24 h before first challenge. therapy reduced eosinophil infiltration, smooth muscle-specific actin expression, subepithelial...
The aim of this study was to test the hypothesis that bone marrow mononuclear cell (BMDMC) therapy led an improvement in lung mechanics and histology endotoxin-induced injury. Twenty-four C57BL/6 mice were randomly divided into four groups ( n = 6 each). In acute injur;y (ALI) group, Escherichia coli lipopolysaccharide (LPS) instilled intratracheally (40 μg, IT), control (C) received saline (0.05 ml, IT). One hour after administration or LPS, BMDMC (2 × 10 7 cells) intravenously injected. At...
The time course of lung mechanics, histology, and inflammatory fibrogenic mediators are analysed after intratracheal instillation (IT) bone marrow-derived cells (BMDC) in a model silicosis. C57BL/6 mice were randomly divided into SIL (silica, 20mg IT) control (CTRL) groups (saline IT). At day 15, received saline or BMDC (2 x 10(6)cells) IT. biodistribution technetium-99m was higher lungs compared with other organs. days 30 60, the area granulomatous nodules, mRNA expression IL-1beta TGF-beta...
We hypothesized that the route of administration would impact beneficial effects bone marrow-derived mononuclear cell (BMDMC) therapy on remodelling process asthma. C57BL/6 mice were randomly assigned to two main groups. In OVA group, sensitized and challenged with ovalbumin, while control group received saline using same protocol. Twenty-four hours before first challenge, animals further randomized into three subgroups receive (SAL), BMDMCs intravenously (2 × 106), or intratracheally 106)....
Abstract Endothelial progenitor cells (EPCs) improve survival and reduce organ failure in cecal ligation puncture-induced sepsis; however, expanded EPCs may represent an even better approach for vascular repair. To date, no study has compared the effects of non-expanded (EPC-NEXP) with those (EPC-EXP) mesenchymal stromal human (MSC-HUMAN) mouse (MSC-MICE) origin experimental sepsis. One day after puncture sepsis induction, BALB/c mice were randomized to receive saline, EPC-EXP, EPC-NEXP,...
Abstract Background Malaria is one of the most critical global infectious diseases. Severe systemic inflammatory diseases, such as cerebral malaria, lead to development cognitive and behavioral alterations, learning disabilities loss memory capacity, well increased anxiety depression. The consequences are profound usually contribute reduce patient’s quality life. There no therapies treat neurological sequelae malaria. Mesenchymal stromal cells (MSCs) may be an alternative, since they have...