A5073885212- Immune Response and Inflammation
- S100 Proteins and Annexins
- Long-Term Effects of COVID-19
- Inflammation biomarkers and pathways
- MicroRNA in disease regulation
- Immune cells in cancer
- Intensive Care Unit Cognitive Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Sepsis Diagnosis and Treatment
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Respiratory Support and Mechanisms
- Mesenchymal stem cell research
- interferon and immune responses
- Epigenetics and DNA Methylation
- Cytokine Signaling Pathways and Interactions
- COVID-19 Clinical Research Studies
- Cardiac Fibrosis and Remodeling
- Heat shock proteins research
- Adipokines, Inflammation, and Metabolic Diseases
- Pain Management and Treatment
- Multiple Sclerosis Research Studies
- Advanced Glycation End Products research
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
St. Michael's Hospital
2015-2025
University of Toronto
2015-2025
The University of Queensland
2023-2025
Ollscoil na Gaillimhe – University of Galway
2024
Unity Health Toronto
2020-2023
Mayo Clinic in Florida
2019-2022
The King's College
2021
The Ohio State University Wexner Medical Center
2021
University of Groningen
2021
University Medical Center Groningen
2021
Sepsis-associated encephalopathy (SAE) is characterized as brain dysfunction associated with sepsis. In this study we sought to investigate the effects of resveratrol in mice SAE, well its NLRP3 inflammasome and IL-1β, which were critical pathogenesis SAE. SAE was induced via cecal ligation puncture (CLP), administered at two doses after surgery. Spatial learning memory functions evaluated by Morris water maze testing. Apoptosis hippocampus quantified using TUNEL assay. Inflammation...
Although mesenchymal stromal (stem) cell (MSC) administration attenuates sepsis-induced lung injury in pre-clinical models, the mechanism(s) of action and host immune system contributions to its therapeutic effects remain elusive. We show that treatment with MSCs decreased expression host-derived microRNA (miR)-193b-5p increased target gene, tight junctional protein occludin (Ocln), lungs from septic mice. Mutating Ocln 3' untranslated region miR-193b-5p binding sequence impaired mRNA....
Oxidative stress is considered one of the early underlying contributors acute lung injury (ALI) and ventilator-induced (VILI). DJ-1, also known as PARK7, has a well-established role an antioxidant. We have previously shown maintaining oxidative balance via ATF3-Nrf2 axis was important in protection from ALI. Here, we exclusively characterize DJ-1 sterile LPS-induced ALI VILI. protein expression increased after LPS treatment human epithelial endothelial cell lines lungs wild-type mice....
Effective and rapid bacterial clearance is a fundamental determinant of outcomes in sepsis. DJ-1 well-established reactive oxygen species (ROS) scavenger.Because cellular ROS status pivotal to inflammation killing, we determined the role sepsis.We used cell murine models with gain- loss-of-function experiments, plasma, cells from patients sepsis.Stimulation bone marrow-derived macrophages (BMMs) endotoxin resulted increased mRNA protein expression. Cellular mitochondrial was DJ-1-deficient...
Epigenetic alterations are an important regulator of gene expression in health and disease; however, epigenetic data sepsis lacking. To demonstrate proof concept estimate effect size, we performed the first epigenome-wide methylation analysis whole blood DNA samples from a cohort septic nonseptic critically ill patients.
Macrophages in the tumor microenvironment respond to complex cytokine signals. How these responses shape phenotype of tumor-associated macrophages (TAMs) is incompletely understood. Here we explored how cytokines milieu, interleukin (IL)-6 and IL-4, interact influence target gene expression primary human monocyte-derived (hMDMs). We show that dual stimulation with IL-4 IL-6 synergistically modified expression. Among induced genes are several targets known pro-tumorigenic properties, such as...
Diabetes Mellitus (DM) is the most common chronic disease. DM considered a Cardiovascular Disease (CVD) risk equivalent. Its macrovascular complications are associated with two-fold increased of premature atherosclerotic CVD. Most diabetics cardiovascular involvement asymptomatic. Electro Cardio Graph (ECG) abnormalities found to be predictors silent ischaemia in asymptomatic persons. An abnormal ECG response statistically significant high for cardiac mortality and morbidity.The aim study...
Arachidonate 15-Lipoxygenase (ALOX15) and arachidonate 15-Lipoxygenase, Type B (ALOX15B) catalyze the dioxygenation of polyunsaturated fatty acids are upregulated in human alternatively activated macrophages (AAMs) induced by Th2 cytokine interleukin-4 and/or interleukin-13. Known primarily for roles bioactive lipid mediator synthesis, 15-lipoxygenases (15-LOXs) have been implicated various macrophage functions including efferocytosis ferroptosis. Using a combination inhibitors siRNAs to...
Virus-induced lung injury is associated with loss of pulmonary epithelial-endothelial tight junction integrity. While the alveolar-capillary membrane may be an indirect target injury, viruses interact directly and/or indirectly miRs to augment their replication potential and evade host antiviral defense system. Here, we expose how influenza virus (H1N1) capitalizes on host-derived interferon-induced, microRNA (miR)-193b-5p occludin compromise defenses. Lung biopsies from patients infected...
Abstract Obesity-associated insulin resistance is driven by inflammatory processes in response to metabolic overload. inflammation can be recapitulated cell culture exposing macrophages saturated fatty acids (SFA) and endoplasmic reticulum (ER) stress responses essentially contribute pro-inflammatory signalling. AMP-activated protein kinase (AMPK) a central regulator with established anti-inflammatory actions. Whether pharmacological AMPK activation suppresses SFA-induced human system...
Abstract The brief lifespan of the polymorphonuclear neutrophil (PMN) is regulated through its capacity to undergo apoptosis, a constitutive process that actively inhibited during sepsis. We sought define cellular mechanisms which Heat Shock Protein 90 (Hsp90) prolongs survival inflammatory PMN. evaluated Hsp90 expression and interaction with client proteins in PMNs from patients sepsis healthy control treated LPS (1 μg/mL). activity was pharmacologically using radicicol (Rad; 1 μM),...
Acute Respiratory Distress Syndrome (ARDS) is characterized by lung inflammation and increased membrane permeability, which represents the leading cause of mortality in ICUs. Mechanical ventilation strategies are at forefront supportive approaches for ARDS. Recently, an increasing understanding RNA biology, function, regulation, as well success vaccines, has spurred enthusiasm emergence novel RNA-based therapeutics. The most common types seen development silencing (si)RNAs, antisense...