A5022205685- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Virus-based gene therapy research
- MicroRNA in disease regulation
- Intensive Care Unit Cognitive Disorders
- Inflammation biomarkers and pathways
- Neonatal Respiratory Health Research
- Graphene and Nanomaterials Applications
- Nanoparticle-Based Drug Delivery
- Respiratory Support and Mechanisms
- Cancer-related molecular mechanisms research
- Sepsis Diagnosis and Treatment
- Autophagy in Disease and Therapy
- Neonatal and Maternal Infections
- Neuroinflammation and Neurodegeneration Mechanisms
- Lipid Membrane Structure and Behavior
- Renal and related cancers
- Retinal Development and Disorders
- Hydrogen's biological and therapeutic effects
- Polymer Surface Interaction Studies
- Renal cell carcinoma treatment
- Retinal Diseases and Treatments
- Lanthanide and Transition Metal Complexes
New Jersey Institute of Technology
2020-2023
St. Michael's Hospital
2018-2023
Unity Health Toronto
2020-2023
University of Toronto
2019
University of Alberta
2016
Utrecht University
2010-2012
RWTH Aachen University
2010
Human umbilical cord mesenchymal stromal cells possess considerable therapeutic promise for acute respiratory distress syndrome. Umbilical may exert effects via extracellular vesicles, while priming further enhance their effect. The authors investigated whether interferon-γ-primed would generate cell-derived vesicles with enhanced in Escherichia coli (E. coli) pneumonia.In a university laboratory, anesthetized adult male Sprague-Dawley rats (n = 8 to 18 per group) underwent intrapulmonary E....
Although mesenchymal stromal (stem) cell (MSC) administration attenuates sepsis-induced lung injury in pre-clinical models, the mechanism(s) of action and host immune system contributions to its therapeutic effects remain elusive. We show that treatment with MSCs decreased expression host-derived microRNA (miR)-193b-5p increased target gene, tight junctional protein occludin (Ocln), lungs from septic mice. Mutating Ocln 3' untranslated region miR-193b-5p binding sequence impaired mRNA....
Oxidative stress is considered one of the early underlying contributors acute lung injury (ALI) and ventilator-induced (VILI). DJ-1, also known as PARK7, has a well-established role an antioxidant. We have previously shown maintaining oxidative balance via ATF3-Nrf2 axis was important in protection from ALI. Here, we exclusively characterize DJ-1 sterile LPS-induced ALI VILI. protein expression increased after LPS treatment human epithelial endothelial cell lines lungs wild-type mice....
N,N,N-Trimethylated chitosan (TMC) is a biodegradable polymer emerging as promising nonviral vector for nucleic acid and protein delivery. In the present study, we investigated whether introduction of thiol groups in TMC enhances extracellular stability complexes based on this promotes intracellular release siRNA. The gene silencing activity cellular cytotoxicity polyplexes thiolated were compared with those nonthiolated counterpart regularly used lipidic transfection agent Lipofectamine....
Virus-induced lung injury is associated with loss of pulmonary epithelial-endothelial tight junction integrity. While the alveolar-capillary membrane may be an indirect target injury, viruses interact directly and/or indirectly miRs to augment their replication potential and evade host antiviral defense system. Here, we expose how influenza virus (H1N1) capitalizes on host-derived interferon-induced, microRNA (miR)-193b-5p occludin compromise defenses. Lung biopsies from patients infected...
To discuss progress in research on choroideremia (CHM) and related retinopathies with special emphasis gene therapy approaches.Biomedical clinical researchers from across the world as well representatives of social science community were convened to 2nd International Scientific Symposium for Choroideremia Denver, Colorado June 2014 enhance our understanding CHM accelerate translation application benefit those affected by CHM.Pre-clinical using cell animal models continues further...
Sepsis-induced myocardial dysfunction (MD) is an important pathophysiological feature of multiorgan failure caused by a dysregulated host response to infection. Patients with MD continue be managed in intensive care units limited understanding the molecular mechanisms controlling disease pathogenesis. Emerging evidences support use mesenchymal stem/stromal cell (MSC) therapy for treating critically ill septic patients. Combining this known role that microRNAs (miRNAs) play reversing...
Oxidative stress is considered one of the early underlying contributors sepsis-induced myocardial depression. DJ-1, also known as PARK7, has a well-established role an antioxidant. We have previously shown, in clinically relevant model polymicrobial sepsis, DJ-1 deficiency improved survival and bacterial clearance by decreasing ROS production. In present study, we investigated Here compared wildtype (WT) with deficient mice at 24 48 h after cecal ligation puncture (CLP). WT mice, was...
ABSTRACT Sunitinib is the standard‐of‐care, first‐line treatment for advanced renal cell carcinoma (RCC). Characteristics of treatment‐resistant RCC have been described; however, complex tumor adaptation mechanisms obstruct identification significant operators in resistance. We hypothesized that resistance a late manifestation early, treatment‐induced histomolecular alterations; therefore, studying early drug response may identify drivers describe an epithelioid growth pattern xenografts,...