A5059860426- Viral Infectious Diseases and Gene Expression in Insects
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Transgenic Plants and Applications
- Microbial Metabolic Engineering and Bioproduction
- 3D Printing in Biomedical Research
- Virus-based gene therapy research
- Metabolomics and Mass Spectrometry Studies
- Gene Regulatory Network Analysis
- Protein purification and stability
- Monoclonal and Polyclonal Antibodies Research
- Advanced Control Systems Optimization
- Mitochondrial Function and Pathology
- CAR-T cell therapy research
- Tissue Engineering and Regenerative Medicine
- Neuroscience and Neural Engineering
- SARS-CoV-2 and COVID-19 Research
- Neuroscience and Neuropharmacology Research
- Pancreatic function and diabetes
- Neurogenesis and neuroplasticity mechanisms
- thermodynamics and calorimetric analyses
- Advanced Sensor and Energy Harvesting Materials
- Anesthesia and Neurotoxicity Research
- Wnt/β-catenin signaling in development and cancer
- Viral gastroenteritis research and epidemiology
ETH Zurich
2017-2025
Hospital Center of Cova da Beira
2019-2024
University of Basel
2024
Westlake University
2024
Instituto de Biologia Experimental e Tecnológica
2010-2021
Universidade Nova de Lisboa
2010-2021
Hospitais da Universidade de Coimbra
2019
Instituto Português Da Qualidade
2007-2011
Rede de Química e Tecnologia
2005-2008
The immature phenotype of human pluripotent stem cell derived cardiomyocytes (hPSC-CMs) constrains their potential in therapy and drug testing. In this study, we report that shifting hPSC-CMs from glucose-containing to galactose- fatty acid-containing medium promotes fast maturation into adult-like CMs with higher oxidative metabolism, transcriptional signatures closer those adult ventricular tissue, myofibril density alignment, improved calcium handling, enhanced contractility, more...
Wearable electronic devices are playing a rapidly expanding role in the acquisition of individuals' health data for personalized medical interventions; however, wearables cannot yet directly program gene-based therapies because lack direct electrogenetic interface. Here we provide missing link by developing an interface that call current (DC)-actuated regulation technology (DART), which enables electrode-mediated, time- and voltage-dependent transgene expression human cells using DC from...
Abstract Three‐dimensional (3D) cultures of human pluripotent stem cell derived cardiomyocytes (hPSC‐CMs) hold great promise for drug discovery, providing a better approximation to the in vivo physiology over standard two‐dimensional (2D) monolayer cultures. However, transition CM differentiation protocols from 2D 3D is not straightforward. In this work, we relied on aggregation hPSC‐derived cardiac progenitors and their culture under agitated conditions generate highly pure cardiomyocyte...
Chinese hamster ovary (CHO) cells are preferred hosts for the production of recombinant biopharmaceuticals. Efforts to optimize these bioprocesses have largely relied on empirical experience and our knowledge cellular behavior in culture is incomplete. More recently, comprehensive investigations metabolic network operation started be used uncover traits associated with optimal growth protein production. In this work, we (1) H-nuclear magnetic resonance ((1) H-NMR) analyze supernatants...
Chinese hamster ovary (CHO) cells are the predominant host for production of therapeutic glycoproteins. In particular, glutamine-synthetase (GS) expression system has been widely used in biopharmaceutical industry efficient selection high-yielding clones. However, much remains unclear on how metabolic wiring affects culture performance. For instance, asparagine and serine have observed to be largest nitrogen sources taken up by GS-CHO cells, but their roles biosynthesis energy generation...
Traceless physical cues are desirable for remote control of the in situ production and real-time dosing biopharmaceuticals cell-based therapies. However, current optogenetic, magnetogenetic, or electrogenetic devices require sophisticated electronics, complex artificial intelligence-assisted software, external energy supplies power control. Here, we describe a self-sufficient subcutaneous push button-controlled cellular implant powered simply by repeated gentle finger pressure exerted on...
Abstract Bacterial transcription factors (TFs) with helix-turn-helix (HTH) DNA-binding domains have been widely explored to build orthogonal transcriptional regulation systems in mammalian cells. Here we capitalize on the modular structure of these proteins a framework for multi-input logic gates relying serial combinations inducible protein–protein interactions. We found that some TFs, their HTH domain alone is sufficient DNA binding. By fusing established dimerization dependent rather than...
The development of technologies to enable fresh water harvesting from atmospheric moisture could help overcome the problem potable scarcity. Here, an (AWH) device is assembled in a core-shell structure, with core consisting networks alginate (Alg) and polyaniline (PANI) outer layer thermo-responsive poly(N-isopropylacrylamide) (PNIPAM) modified sulfonic acid groups (SPNIPAM) increase adsorption at low relative humidity. resulting hydrogel, lithium chloride (LiCl) for increased storage...
Abstract Despite the array of mammalian transgene switches available for regulating therapeutic protein expression in response to small molecules or physical stimuli, issues remain, including cytotoxicity chemical inducers and limited biocompatibility cues. This study introduces gene driven by short peptides comprising eight fewer amino acid residues. Utilizing a competence regulator (ComR) sigma factor X‐inducing peptide (XIP) from Streptococcus vestibularis as receptor inducer,...
Abstract Background The progress in the "-omic" sciences has allowed a deeper knowledge on many biological systems with industrial interest. This is still rarely used for advanced bioprocess monitoring and control at bioreactor level. In this work, method presented, which designed basis of metabolic network organism under consideration. dynamics are formulated using hybrid rigorous/data driven its inherent structure defined by metabolism elementary modes. Results system study decomposed into...
Abstract The main objective of the present study was to investigate use in situ 2D fluorometry for monitoring key bioprocess variables mammalian cell cultures, namely concentration viable cells and recombinant proteins. All studies were conducted using a Baby Hamster Kidney (BHK) line expressing fusion glycoprotein IgG1‐IL2 cultured batch fed‐batch modes. It observed that intensity fluorescence signals excitation/emission wavelength range amino acids, vitamins NAD(P)H changed along culture...
Abstract Precise control of the delivery therapeutic proteins is critical for gene- and cell-based therapies, expression should only be switched on in presence a specific trigger signal appropriate magnitude. Focusing advantages delivering by inhalation, we have developed mammalian synthetic gene switch that enables regulation transgene exposure to semi-volatile small molecule acetoin, widely used, FDA-approved food flavor additive. The capitalizes bacterial regulatory protein AcoR fused...
Abstract Viral proteases and clinically safe inhibitors were employed to build integrated compact regulators of protein activity (iCROP) for post-translational regulation functional proteins by tunable proteolytic activity. In the absence inhibitor, co-localized/fused protease cleaves a target peptide sequence introduced in an exposed loop interest, irreversibly fragmenting structure destroying its functionality. We selected three demonstrated versatility iCROP framework validating it...
Abstract This report highlights the potential of measurement, monitoring, modeling and control (M 3 C) methodologies in animal human cell culture technology. In particular, state‐of‐the‐art M C technologies their industrial relevance existing technology are addressed. It is a summary an expert panel discussion between biotechnologists biochemical engineers with both academic backgrounds. The latest ascents discussed from perspective for process development production needs. concludes set...
Abstract Background Stoichiometric models constitute the basic framework for fluxome quantification in realm of metabolic engineering. A recurrent bottleneck, however, is establishment consistent stoichiometric synthesis recombinant proteins or viruses. Although optimization algorithms silico redesign have been developed context genome-scale small molecule production, still rudimentary knowledge how different cellular levels are regulated and phenotypically expressed prevents their full...
Abstract Body temperature is maintained at around 37 °C in humans, but may rise to 40 or more during high‐grade fever, which occurs most adults who are seriously ill. However, endogenous sensors, such as ion channels and heat‐shock promoters, fully activated only noxious temperatures above this range, making them unsuitable for medical applications. Here, a genetically encoded protein thermometer (human enhanced gene activation thermometer; HEAT) designed that can trigger transgene...
Abstract Synthetic biology aims to engineer transgene switches for precise therapeutic protein control in cell‐based gene therapies. However, off‐the‐shelf trigger‐inducible circuits are usually switched on by single or structurally similar molecules. This study presents a mammalian gene‐switch platform that controls expression wide range of molecules generating low, non‐toxic levels reactive oxygen species (ROS). In this system, KEAP1 (Kelch‐like ECH‐associated 1) serves as ROS sensor,...