A5066931114- CRISPR and Genetic Engineering
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 and COVID-19 Research
- Viral Infectious Diseases and Gene Expression in Insects
- Cellular transport and secretion
- Endoplasmic Reticulum Stress and Disease
- Pancreatic function and diabetes
- Lipid Membrane Structure and Behavior
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Animal Genetics and Reproduction
- Virus-based gene therapy research
ETH Zurich
2021-2025
National Institute of Chemistry
2021-2022
Abstract Secreted proteins, such as hormones or cytokines, are key mediators in multicellular organisms. Response of protein secretion based on transcriptional control is rather slow, it requires transcription, translation and transport from the endoplasmic reticulum (ER) to plasma membrane via conventional (CPS) pathway. An alternative regulation provide faster response would be valuable. Here we present two genetically encoded orthogonal regulatory systems, which rely retention...
Abstract Viral proteases and clinically safe inhibitors were employed to build integrated compact regulators of protein activity (iCROP) for post-translational regulation functional proteins by tunable proteolytic activity. In the absence inhibitor, co-localized/fused protease cleaves a target peptide sequence introduced in an exposed loop interest, irreversibly fragmenting structure destroying its functionality. We selected three demonstrated versatility iCROP framework validating it...
ABSTRACT Human pluripotent stem cells (hPSCs) can be differentiated in vitro to an increasing number of mature cell types, presenting significant promise for addressing a wide range diseases and studying human development. One approach further enhance differentiation methods would coordinate multiple inducible gene or protein switches operate simultaneously within the same cell, with minimal cross‐interference, precisely regulate network lineage‐specifying transcription factors (TFs) guide...
The development of novel strategies to program cellular behaviors is a central goal in synthetic biology, and post-translational control mediated by engineered protein circuits particularly attractive approach achieve rapid secretion on demand. We have developed programmable protease-mediated switch (POSH) platform composed chimeric unit that consists interest fused via transmembrane domain cleavable ER-retention signal, together with two cytosolic inducer-sensitive split protease...
Abstract The main (Mpro) and papain-like (PLpro) proteases encoded by SARS-CoV-2 are essential to process viral polyproteins into functional units, thus representing key targets for anti-viral drug development. There is a need an efficient inhibitor screening system that can identify candidates in cellular context. Here we describe modular, tunable autoproteolytic gene switches (TAGS) relying on synthetic transcription factors self-inactivate, unless the presence of coronavirus protease...
Abstract Secreted proteins, such as hormones or cytokines, are key mediators in multicellular organisms. Protein secretion based on transcriptional control is rather slow, proteins requires transcription, translation, followed by the transport from endoplasmic reticulum (ER) through conventional protein (CPS) pathway towards plasma membrane. An alternative faster bypass would be valuable. Here we present two genetically encoded orthogonal systems, which rely retention of pre-synthesized ER...