A5035206605- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Immune Response and Inflammation
- Trypanosoma species research and implications
- Immunotherapy and Immune Responses
- Research on Leishmaniasis Studies
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Viral Infections and Immunology Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Asthma and respiratory diseases
- COVID-19 Clinical Research Studies
- Protein purification and stability
- Plant Pathogenic Bacteria Studies
- Systemic Lupus Erythematosus Research
- Bioactive Compounds and Antitumor Agents
- Vector-borne infectious diseases
- Diabetes and associated disorders
- Immune cells in cancer
- Plant-Microbe Interactions and Immunity
- Synthesis and Biological Evaluation
- Atherosclerosis and Cardiovascular Diseases
- Allergic Rhinitis and Sensitization
- Mosquito-borne diseases and control
Universidade Federal do Rio de Janeiro
2014-2025
Universidade Federal Fluminense
2024
AC Camargo Hospital
2013
Czech Academy of Sciences, Institute of Microbiology
2010
Universidade do Estado do Rio de Janeiro
2010
Japan Science and Technology Agency
2005
Osaka University
2005
Institut Pasteur
1993-2002
Centre National de la Recherche Scientifique
1993-1998
Cornell University
1996
Abstract TLRs function as pattern recognition receptors in mammals and play an essential role the of microbial components. We found that injection glycoinositolphospholipids (GIPLs) from Trypanosoma cruzi into peritoneal cavity mice induced neutrophil recruitment a TLR4-dependent manner: GIPL TLR4-deficient strain (C57BL/10ScCr) caused no inflammatory response. In contrast, TLR2 knockout mice, chemoattraction did not differ significantly seen wild-type controls. GIPL-induced attraction MIP-2...
Antigen-free (AGF) and germ-free (GF) mice, although essentially free of serum IgG, maintain normal levels circulating IgM. Using a quantitative immunoblot assay, we have now analyzed the repertoire IgM from AGF, GF, specific pathogen-free (SPF) BALB/c on large panels natural antigens homologous tissues bacteria. The reactivity profiles were very similar in three groups mice. Multiparametric statistic evaluation data showed that animals, SPF, AGF mice constitute an homogeneous group with...
Hemocytes are the first line of defense immune system in invertebrates, but despite their important role and enormous potential for study gene-environment relationships, research has been impeded by a lack consensus on classification. Here we used flow cytometry combined with histological procedures, histochemical reactions transmission electron microscopy to characterize hemocytes from oyster Crassostrea rhizophorae. Transmission revealed remarkable morphological characteristics, such as...
Analysis of the reactivity IgM with self-antigens in tissues by a quantitative immunoblotting technique showed striking invariance among newborns human and mouse. The self-reactive repertoire adults was also markedly conserved; it comprised most anti-self reactivities that prevailed neonates. Multivariate analysis confirmed homogeneity repertoires neonates toward self- non-self-antigens. discriminated between newborn adult for two five sources self-proteins Our observations support concept...
Abstract The serum IgM repertoires of C57BL/6, DBA/2 and BALB/c mouse strains were analyzed using a recently developed global quantitative assay that measures antibody reactivities to very large number antigens. A characteristic repertoire could be assigned each strain. different successfully classified with multivariate statistics. Many common also observed among the strains, which allows definition mouse‐specific repertoire. Analysis human sera support this notion. To investigate impact...
We investigated the role of Fas ligand in murine silicosis. Wild-type mice instilled with silica developed severe pulmonary inflammation, local production tumor necrosis factor (TNF)-α, and interstitial neutrophil macrophage infiltration lungs. Strikingly, ligand–deficient generalized lymphoproliferative disease mutant (gld) did not develop The gld had markedly reduced extravasation into bronchoalveolar space, show increased TNF-α production, nor inflammation. Bone marrow chimeras adoptive...
ABSTRACT Paenibacillus azotofixans is a nitrogen-fixing bacterium often found in soil and the rhizospheres of different grasses. In this study, two Brazilian clay soils were planted with cross-hybrid maize (BR-201) four stages plant growth analyzed to characterize P. populations present rhizoplanes, rhizospheres, non-root-associated (herein called nonrhizospheres). A total 106 strains isolated identified as an API 50CH kit, by classical biochemical tests, via use specific primers based on...
This paper describes a procedure for analysing multiple antibody reactivities that explores commercially available immunoblot system, and is based on double staining of nitrocellulose membranes, revealing both the migration position blotted proteins in membrane. Quantification stainings by densitometry allowed accurate superposition immunoreactivity total protein profiles each Line. Moreover, ofthe different lanes could be adjusted with simple‐scale transformation algorithm, correcting...
The murine model of T. cruzi infection has provided compelling evidence that development host resistance against intracellular protozoans critically depends on the activation members Toll-like receptor (TLR) family via MyD88 adaptor molecule. However, possibility TLR/MyD88 signaling pathways also control induction immunoprotective CD8+ T cell-mediated effector functions not been investigated to date. We addressed this question by measuring frequencies IFN-gamma secreting cells specific for...
Abstract IL-4 plays an essential role in the activation of mature B cells, but less is known about cell maturation and tolerance checkpoints. In this study, we analyzed effect on vitro maturation, from immature to transitional stages, its influence BCR-mediated negative selection. Starting either purified CD19+IgM− precursors, or sorted bone marrow (B220lowIgMlowCD23−) (B220intIgMhighCD23−) cells C57BL/6 mice, compared effects BAFF. We found that stimulated generation CD23+ CD23− was...
The authors have used a quantitative immunoblotting technique to analyse the antibody repertoire of IgM in cord blood and serum young children, adult males aged directed towards antigens homologous tissues utilized as sources self antigens. reactivities with exhibited striking homogeneity invariance among newborns. Self‐reactive repertoires adults were markedly conserved individuals comprised most anti‐self that prevailed neonates. Reactivities bacterial showed high degree newborns but more...
Abstract The role of TLR4 in mature B cell activation is well characterized. However, little known about development. Here, we analyzed the effects and TLR2 agonists on development using an vitro model maturation. Highly purified B220+IgM− precursors from normal C57BL/6 mouse were cultured for 72 h, maturation presence TLR was evaluated by expression IgM, IgD, CD23, AA4. addition LPS or lipid A resulted a marked increase percentage CD23+ cells, while Pam3Cys had no effect alone, but...
Parkinson's disease (PD) is a progressive degenerative of the central nervous system associated with neuroinflammation and microglial cell activation. Chemokine signaling regulates neuron-glia communication triggers inflammatory profile. Herein, we identified neuronal chemokine CCL21 as major cause imbalance through CCR7 receptor pathway therapeutic implications for PD. In humans, found that transcript expression was increased in dopaminergic neurons (DANs) substantia nigra PD patients....
Lipopolysaccharide (LPS) from gramnegative bacteria is a well-known T cell-independent B lymphocyte mitogen and macrophage/monocyte activator. While the conventional view holds that LPS ignored by cells, we report here administration of to mice activates all but also engages most CD4 CD8 as measured expression activation markers CD69 CD25 size increase. cells recruited in endotoxin-treated showed, following vitro stimulation concanavalin A, altered patterns cytokine production. In vivo,...
Journal Article Analysis of natural and disease-associated autoantibody repertoires: anti-endothelial cell IgG activity in the serum healthy individuals patients with systemic lupus erythematosus Get access Nicoletta Ronda, Ronda Search for other works by this author on: Oxford Academic PubMed Google Scholar Mattias Haury, Haury 1Unité d'lmmunobiologie, CNRS URA 359, Institut Pasteur28 rue du Dr-Roux, 75015 Paris, France Alberto Nobrega, Nobrega Srinivas V. Kaveri, Kaveri Antonio Coutinho,...
Acute graft-versus-host disease (aGVHD) is the main complication of allogeneic hematopoietic stem cell transplantation, and many efforts have been made to overcome this important limitation. We showed previously that G-CSF treatment generates low-density splenic granulocytes inhibit experimental aGVHD. In article, we show aGVHD protection relies on incoming IL-10+ neutrophils from G-CSF-treated donor spleen (G-Neutrophils). These G-Neutrophils high phagocytic capacity, peroxide production,...
Abstract The SARS-CoV-2 pandemic highlighted the urgent need for innovative methods to study humoral immune responses and identify monoclonal antibodies (mAbs) with diagnostic therapeutic potential. Memory B cells (MBCs), pivotal adaptive immunity, generate high-affinity upon antigen re-encounter. While single-cell high-throughput sequencing has revolutionized antibody repertoire studies, it critical limitations: inability simultaneously determine antigen-binding specificities immunoglobulin...
Este trabalho teve como objetivo apresentar a proposta de harmonização da bebida fermentada à base Camellia sinensis (Kombucha), em conjunto com dois bolos típicos Pernambucanos, o bolo rolo e macaxeira. Foram realizadas oficinas comunidades rurais associação pequenos agricultores, Centro Atenção Psicossocial Associação moradores do município Cabo Santo Agostinho, Região Metropolitana Recife. A produção kombucha, uma origem oriental, é obtida através fermentação chá utilizando cultura...
Abstract In the absence of intentional immunizations, normal mice produce natural antibodies that react with a variety self and foreign antigens. We have now addressed putative physiological selection such reactivities some their clonal characteristics, by analyzing produced B cells at different stages differentiation. Using an antigen‐specific spot‐enzyme‐linked immunosorbent assay (ELISA) panel antigens, we found newly formed cells, either from adult bone marrow or newborn spleen, contain...
Germline TP53 mutations predispose to multiple cancers defining Li-Fraumeni/Li-Fraumeni-like syndrome (LFS/LFL), a disease with large individual disparities in cancer profiles and age of onset. G-quadruplexes (G4s) are secondary structural motifs occurring guanine tracks, regulatory effects on DNA RNA. We analyzed 85 polymorphisms within or near five predicted G4s search modifiers penetrance LFS/LFL Brazilian families (n = 35) without 110) mutations. Statistical analyses stratified family...
Selection and physiological production of protective natural antibodies (NAbs) have been associated with exposure to endogenous antigens. The extent which this association depends on germline NAb sequence is uncertain. Here we show that alterations in D(H) can sever the between self-reactive NAbs afford protection against a pathogen. In unmanipulated hosts, availability evolutionarily conserved DFL16.1 gene segment profoundly affected serum levels bacterial phosphorylcholine but not oxidized...
Increasing attention has been directed to cytotoxic CD4 + T cells (CD4CTLs) in different pathologies, both humans and mice. The impact of CD4CTLs immunity the mechanisms controlling their generation, however, remain poorly understood. Here, we show that abundantly differentiate during mouse infection with intracellular parasite Trypanosoma cruzi . display parallel kinetics Th1 spleen, mediate specific cytotoxicity against presenting pathogen-derived antigens express immunoregulatory and/or...