A5015436060- Parkinson's Disease Mechanisms and Treatments
- Microbial Applications in Construction Materials
- Alzheimer's disease research and treatments
- Nerve injury and regeneration
- Neuroinflammation and Neurodegeneration Mechanisms
- Botulinum Toxin and Related Neurological Disorders
- Enzyme Production and Characterization
- Neurological disorders and treatments
- Enzyme Catalysis and Immobilization
- Peptidase Inhibition and Analysis
- Lipid Membrane Structure and Behavior
- Corrosion Behavior and Inhibition
- Histone Deacetylase Inhibitors Research
- Electrochemical sensors and biosensors
- Enzyme-mediated dye degradation
- Bioactive Compounds and Antitumor Agents
- Toxin Mechanisms and Immunotoxins
- biodegradable polymer synthesis and properties
- Synthesis and Biological Evaluation
- Viral Infections and Outbreaks Research
- Antimicrobial Peptides and Activities
- Biochemical and Molecular Research
- Cellular transport and secretion
- Biochemical and Structural Characterization
- Microbial metabolism and enzyme function
Universidade Federal do Rio de Janeiro
2012-2025
Cornell University
2015
Instituto de Biofísica Carlos Chagas Filho
2006
Universidade Federal do Rio Grande do Sul
2001-2004
In this work we compared two plant ureases, jackbean urease (JBU) and embryo‐specific soybean (SBU) a bacterial ( Bacillus pasteurii ) urease, for kinetic parameters other biological properties described recently ureases that are independent of the ureolytic activity. The insecticidal effect was investigated in feeding trials with cotton sucker bug, Dysdercus peruvianus (Hemiptera) as an insect model. Contrasting B. (PBU), both presented potent activity, LD 50 values 0.017% (w/w) 0.052% JBU...
Fibrillization of the protein α-synuclein (α-syn) is a hallmark Parkinson's disease and other α-synucleinopathies. The well-established idea that α-syn natively disordered monomer prone to forming fibrils was recently challenged by data showing mostly exists in vitro vivo as helically folded tetramers are resistant fibrillization. These apparently conflicting findings may be reconciled equilibrium with variable amounts dynamic oligomeric species. In this context, varying approaches used for...
Canatoxin is a toxic protein from Canavalia ensiformis seeds, lethal to mice (LD50 = 2mg/kg) and insects. Further characterization of canatoxin showed that its main native form (184kDa) non-covalently linked dimer 95kDa polypeptide containing zinc nickel. Partial sequencing internal peptides indicated homology with urease (EC 3.5.1.5) the same seed. has approx. 30% urease's activity for urea, Km 2–7mM. The proteins differ in their affinities metal ions were separated by affinity...
In the last decades, a series of compounds, including quinones and polyphenols, has been described as having anti-fibrillogenic action on α-synuclein (α-syn) whose aggregation is associated to pathogenesis Parkinson's disease (PD). Most these molecules act promiscuous anti-amyloidogenic agents, interacting with diverse amyloidogenic proteins (mostly unfolded) through non-specific hydrophobic interactions. Herein we investigated effect vitamins K (phylloquinone, menaquinone menadione), which...
Ureases are metalloenzymes involved in defense mechanisms plants. The insecticidal activity of Canavalia ensiformis (jack bean) ureases relies partially on an internal 10kDa peptide generated by enzymatic hydrolysis the protein within susceptible insects. A recombinant version this peptide, jaburetox, exhibits insecticidal, antifungal and membrane-disruptive properties. Molecular modeling jaburetox revealed a prominent β-hairpin motif consistent with either neurotoxicity or pore...
Antigen B (AgB) is the major protein secreted by Echinococcus granulosus metacestode and involved in key host-parasite interactions during infection. The full comprehension of AgB functions depends on elucidation several structural aspects that remain unknown, such as its subunit composition oligomeric states.The E. oligomers from individual bovine human cysts was assessed mass spectrometry associated with electrophoretic analysis. AgB8/1, AgB8/2, AgB8/3 AgB8/4 subunits were identified all...
Identifying the mechanisms by which presynaptic protein α-synuclein (aSyn) is associated with neurodegeneration of dopamine neurons a major priority in Parkinson's disease (PD) field. Studies indicate that DOPAL (3,4-dihydroxyphenylacetaldehyde), an aldehyde generated from enzymatic oxidation dopamine, may convert aSyn monomer into neurotoxin via formation covalently stabilized toxic oligomers. Herein we investigated role N-terminal acetylation and familial mutations (A30P, A53T, E46K, G51D,...
Canatoxin is a toxic protein from Canavalia ensiformis seeds, lethal to mice (LD50 = 2mg/kg) and insects. Further characterization of canatoxin showed that its main native form (184kDa) non-covalently linked dimer 95kDa polypeptide containing zinc nickel. Partial sequencing internal peptides indicated homology with urease (EC 3.5.1.5) the same seed. has approx. 30% urease's activity for urea, Km 2–7mM. The proteins differ in their affinities metal ions were separated by affinity...
Jaburetox-2Ec, a recombinant peptide derived from an urease isoform (JBURE-II), displays high insecticidal activity against important pests such as Spodoptera frugiperda and Dysdercus peruvianus. Although the molecular mechanism of action ureases-derived peptides remains unclear, previous ab initio data suggest presence structural motifs in Jaburetox-2Ec with characteristics similar to those found class pore-forming peptides. Here, we investigated aspects interaction between large...
Monoamine oxidase (MAO) catalyzes the oxidative deamination of biogenic and exogenous amines its inhibitors have therapeutic value for several conditions including affective disorders, stroke, neurodegenerative diseases aging. The discovery 2,3,6-trimethyl-1,4-naphthoquinone (TMN) as a nonselective reversible inhibitor MAO, has suggested 1,4-naphthoquinone (1,4-NQ) potential scaffold designing new MAO inhibitors. Combining molecular modeling tools biochemical assays we evaluate kinetic...
Parkinson's disease (PD) is a progressive degenerative of the central nervous system associated with neuroinflammation and microglial cell activation. Chemokine signaling regulates neuron-glia communication triggers inflammatory profile. Herein, we identified neuronal chemokine CCL21 as major cause imbalance through CCR7 receptor pathway therapeutic implications for PD. In humans, found that transcript expression was increased in dopaminergic neurons (DANs) substantia nigra PD patients....
This study investigates the potential of arimoclomol-loaded nanomicelles for treatment neurodegenerative diseases like Alzheimer's and Parkinson's, as well their anti-inflammatory properties. Arimoclomol, a coinducer heat shock proteins (HSPs), has shown clinical promise in mitigating protein misfolding, hallmark these diseases. In this work, arimoclomol significantly reduced aggregation β-amyloid (Aβ1–42) α-synuclein (α-syn), key pathological Parkinson's. Additionally, demonstrated potent...
Background/Objectives: Lipoxins, particularly Lipoxin A4 (LXA4), are endogenous lipid mediators with potent anti-inflammatory and pro-resolving properties, making them promising candidates for the treatment of inflammatory neurodegenerative disorders. However, their therapeutic application is limited by poor stability bioavailability. This study aimed to develop characterize nanomicelles encapsulating LXA4 (nano-lipoxin A4) improve its pharmacological efficacy against Alzheimer's disease...
Parkinson's disease (PD) is characterized by the presence of cytoplasmic inclusions composed R-synuclein (R-syn) in dopaminergic neurons.This suggests a pivotal role dopamine (DA) on PD development.Here, we show that DA modulates differently stability protofibrils (PF) and fibrils (F) wild type or variants R-syn (A30P A53T) as probed high hydrostatic pressure (HHP).While absence DA, all PF exhibited identical stability, its presence, variantcomposed acquired greater (DAPF wt < DAPF A30P )...