A5060463130- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- Legionella and Acanthamoeba research
- Viral gastroenteritis research and epidemiology
- Parkinson's Disease Mechanisms and Treatments
- Virus-based gene therapy research
- Mosquito-borne diseases and control
- Bacterial Genetics and Biotechnology
- Alzheimer's disease research and treatments
- Animal Virus Infections Studies
- Heme Oxygenase-1 and Carbon Monoxide
- Genomics and Phylogenetic Studies
- Viral Infections and Vectors
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Vibrio bacteria research studies
- Cancer Research and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Connexins and lens biology
- Bacterial biofilms and quorum sensing
- Virology and Viral Diseases
- HIV Research and Treatment
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Neurological diseases and metabolism
- CRISPR and Genetic Engineering
Universidade Federal do Rio de Janeiro
2014-2024
University of Connecticut
2010-2024
University of Alabama at Birmingham
2007-2023
Fundação Oswaldo Cruz
2023
Universidade Estadual de Campinas (UNICAMP)
2023
Instituto Nacional da Propriedade Industrial
2023
Czech Academy of Sciences, Institute of Microbiology
2014
Universidad de Málaga
2012
Masimo (United States)
2012
Montgomery College
2012
Fibrillization of the protein α-synuclein (α-syn) is a hallmark Parkinson's disease and other α-synucleinopathies. The well-established idea that α-syn natively disordered monomer prone to forming fibrils was recently challenged by data showing mostly exists in vitro vivo as helically folded tetramers are resistant fibrillization. These apparently conflicting findings may be reconciled equilibrium with variable amounts dynamic oligomeric species. In this context, varying approaches used for...
ABSTRACT Acanthamoeba polyphaga mimivirus (APMV) is a giant virus from the Mimiviridae family. It has many unusual features, such as pseudoicosahedral capsid that presents starfish shape in one of its vertices, through which ∼1.2-Mb double-stranded DNA released. also dense glycoprotein fibril layer covering not yet been functionally characterized. Here, we verified although these structures are essential for viral replication, they truly necessary adhesion to amoebae, natural host. In...
Since their discovery, giant viruses have expanded our understanding of the principles virology. Due to gargantuan size and complexity, little is known about life cycles these viruses. To answer outstanding questions regarding virus infection mechanisms, we set out determine biomolecular conditions that promote genome release. We generated four intermediates in Samba (Mimivirus genus, lineage A) as visualized by cryoelectron microscopy (cryo-EM), tomography (cryo-ET), scanning electron...
Since the discovery of giant viruses infecting amoebae in 2003, many dogmas virology have been revised and search for these has intensified. Over last few years, several new groups discovered various types samples environments.In this work, we describe isolation 68 amoeba obtained from environmental Brazil Antarctica. Isolated were identified by hemacolor staining, PCR assays electron microscopy (scanning and/or transmission). A total 64 belonging to Mimiviridae family isolated (26 lineage...
Acanthamoeba castellanii (Ac) are ubiquitously distributed in nature, and by contaminating medical devices such as heart valves contact lenses, they cause a broad range of clinical presentations to humans. Although several molecules have been described play role Ac pathogenesis, including parasite host-tissue invasion escaping host-defense, little information is available on their mechanisms secretion. Herein, we describe the molecular components secreted Ac, under different protein...
A novel role for scaffolding protein in portal ring formation.
Free-living amoebae (FLAs) are major reservoirs for a variety of bacteria, viruses, and fungi. The most studied mycophagic FLA, Acanthamoeba castellanii (Ac), is potential environmental host endemic fungal pathogens such as Cryptococcus spp., Histoplasma capsulatum, Blastomyces dermatitides, Sporothrix schenckii. However, the mechanisms involved in this interaction poorly understood. aim work was to characterize molecular instances that enable Ac interact with ingest pathogens, process could...
ABSTRACT Icosahedral virus assembly requires a series of concerted and highly specific protein-protein interactions to produce proper capsid. In bacteriophage P22, only coat protein (gp5) scaffolding (gp8) are needed assemble procapsid-like particle, both in vivo vitro . protein's binding domain, residue R293 is required for procapsid assembly, while K296 important but not essential. Here, we investigate the interaction with acidic residues N-arm protein, since this has been shown be...
Oxidative stress and the formation of cytotoxic aggregates presynaptic protein α-synuclein (AS) are two important events associated with pathogenesis Parkinson's disease (PD) several other neurodegenerative diseases. In this context, extensive efforts have been made to elucidate molecular basis synergy between oxidative AS aggregation. study, we demonstrate that exposure induced by UV radiation (ASUV) blocks fibrillation, leading highly toxic fibril-incompetent oligomers. addition, ASUV...
Prior to the discovery of mimivirus in 2003, viruses were thought be physically small and genetically simple. Mimivirus, with its ~750-nm particle size ~1.2-Mbp genome, shattered these notions changed what it meant a virus. Since this discovery, isolation characterization giant has exploded. One more recently discovered viruses, Samba virus, is Mimivirus that was isolated from Rio Negro Brazilian Amazon. Initial revealed some structural information, although preparation techniques used are...
The inclusion of Mimiviridae members in the putative monophyletic nucleocytoplasmic large DNA virus (NCLDV) group is based on genomic and phylogenomic patterns. This shows that, along with other viral families, they share a set genes known as core or "hallmark genes," including gene for major capsid protein (MCP). Although previous studies have suggested that maturation mimivirus MCP transcripts dependent splicing, there little information about processing this transcript isolates. Here we...
ABSTRACT Tupanviruses, members of the family Mimiviridae , infect phagocytic cells. Particle uncoating begins inside phagosome, with capsid opening via stargate. The mechanism through which this takes place is unknown. Once phagocytized, metal ion flux control and ROS are induced to inactivate foreign particles, including viruses. Here, we studied effect iron ions, copper H 2 O on Tupanvirus particles. Such treatments stargate in vitro as observed by different microscopy techniques....