A5002168941- Herpesvirus Infections and Treatments
- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- interferon and immune responses
- Mosquito-borne diseases and control
- Parasites and Host Interactions
- Toxoplasma gondii Research Studies
- Viral Infections and Vectors
- Influenza Virus Research Studies
- Poxvirus research and outbreaks
- Immune Response and Inflammation
- Malaria Research and Control
- Immune Cell Function and Interaction
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Systemic Lupus Erythematosus Research
- Long-Term Effects of COVID-19
- Pneumonia and Respiratory Infections
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- Infectious Diseases and Mycology
- Cytokine Signaling Pathways and Interactions
- Viral Infectious Diseases and Gene Expression in Insects
- Viral Infections and Immunology Research
Fundação Oswaldo Cruz
2016-2025
University of New Mexico
2023
University of Évora
2020
Universidade Federal de Minas Gerais
1998-2019
Instituto Butantan
2019
Companhia Energética de Minas Gerais (Brazil)
2012
Centro de Pesquisas Oncológicas
2011
Texas Medical Board
2002
University of Würzburg
1993
Abstract Glycosylphosphatidylinositol (GPI) anchors and glycoinositolphospholipids (GIPLs) from parasitic protozoa have been shown to exert a wide variety of effects on cells the host innate immune system. However, receptor(s) that are triggered by these protozoan glycolipids has not identified. Here we present evidence Trypanosoma cruzi-derived GPI GIPLs trigger CD25 expression Chinese hamster ovary-K1 transfected with CD14 Toll-like receptor-2 (TLR-2), but wild-type (TLR-2-deficient) ovary...
Abstract GPIs isolated from Toxoplasma gondii, as well a chemically synthesized GPI lacking the lipid moiety, activated reporter gene in Chinese hamster ovary cells expressing TLR4, while core glycan and moieties cleaved both TLR4- TLR2-expressing cells. MyD88, but not TLR2, or CD14, is absolutely needed to trigger TNF-α production by macrophages exposed T. gondii GPIs. Importantly, response was completely abrogated TLR2/4-double-deficient mice. MyD88−/− mice were more susceptible death than...
Abstract TLRs function as pattern recognition receptors in mammals and play an essential role the of microbial components. We found that injection glycoinositolphospholipids (GIPLs) from Trypanosoma cruzi into peritoneal cavity mice induced neutrophil recruitment a TLR4-dependent manner: GIPL TLR4-deficient strain (C57BL/10ScCr) caused no inflammatory response. In contrast, TLR2 knockout mice, chemoattraction did not differ significantly seen wild-type controls. GIPL-induced attraction MIP-2...
Abstract Studies performed in vitro suggest that activation of Toll-like receptors (TLRs) by parasite-derived molecules may initiate inflammatory responses and host innate defense mechanisms against Trypanosoma cruzi. Here, we evaluated the impact TLR2 myeloid differentiation factor 88 (MyD88) deficiencies resistance to infection with T. Our results show macrophages derived from −/− MyD88−/− mice are less responsive GPI-mucin cruzi trypomastigotes parasites. In contrast, same cells TLR2−/−...
Trypomastigote forms of Trypanosoma cruzi, the causative agent Chagas Disease, shed extracellular vesicles (EVs) enriched with glycoproteins gp85/trans-sialidase (TS) superfamily and other α-galactosyl (α-Gal)-containing glycoconjugates, such as mucins. Here, purified from T. cruzi strains (Y, Colombiana, CL-14 YuYu) were quantified according to size, intensity concentration. Qualitative analysis revealed differences in their protein contents. Later, those polymorphisms evaluated modulation...
The immunomodulatory properties of lipophosphoglycans (LPG) from New World species Leishmania have been assessed in infantum and braziliensis, the causative agents visceral cutaneous leishmaniasis, respectively. This glycoconjugate is highly polymorphic among with variation sugars that branch off conserved Gal(β1,4)Man(α1)-PO4 backbone repeat units. Here, activity LPGs amazonensis, agent diffuse was evaluated two strains Brazil. One strain (PH8) originally isolated sand fly other (Josefa) a...
Extracellular vesicles (EVs) shed by trypomastigote forms of Trypanosoma cruzi have the ability to interact with host tissues, increase invasion, and modulate innate response. In this study, EVs from T. or T.cruzi-infected macrophages were investigated as immunomodulatory agents during initial steps infection. Initially, scanning electron microscopy nanoparticle tracking analysis, we determined that cruzi-infected released higher numbers (50-300 nm) compared noninfected cells. Using...
ABSTRACT Initial host defense to bacterial infection is executed by innate immunity, and therefore the main goal of this study was examine contribution Toll-like receptors (TLRs) during Brucella abortus infection. CHO reporter cell lines transfected with CD14 TLRs showed that B. triggers both TLR2 TLR4. In contrast, lipopolysaccharide (LPS) lipid A derived from rough (R) smooth (S) strains activate cells only through Consistently, macrophages C3H/HePas mice exposed R S their LPS produced...
Abstract Background The dominant, cell surface lipophosphoglycan (LPG) of Leishmania is a multifunctional molecule involved in the interaction with vertebrate and invertebrate hosts. Although role LPG on infection has been extensively studied, it not known if interspecies variations contribute to different immunopathologies leishmaniases. To investigate issue polymorphisms, two species from New World that express structural side chains repeat units were examined. In this context, procyclic...
Abstract Background Dengue, one of the most important arboviral diseases humans, may cause severe systemic disease. Although dengue virus (DENV) has been considered to be a non-neurotropic virus, infection associated recently with series neurological syndromes, including encephalitis. In this work, we evaluated behavioral changes and inflammatory parameters in C57BL/6 mice infected non-adapted 3 (DENV-3) genotype I. Methods received 4 × 10 PFU DENV-3 by an intracranial route. We trafficking...
Herpes simplex virus type 1 (HSV-1) cause not only mild symptoms but also blindness and encephalitis. It was previously shown that the immune response against HSV-1 occurs mainly in trigeminal ganglia (TG) Toll-like receptors 2 9 (TLR2/9) are important mediating this response. demonstrated iNOS (nitric oxide synthase) interleukin beta (IL-1β) play an essential role defense infection. Importantly, present work aimed to identify primary cells responsible for IL-1β production search other...
Bovine vaccinia virus outbreaks have been occurring in different regions of Brazil. We report here the time course natural human infection by and describe important clinical epidemiological aspects this zoonotic infection. The diagnosis was based on clinical, serological, molecular procedures.
The Herpes simplex virus ‐1 (HSV‐1) is responsible for several clinical manifestations in humans, including encephalitis. To induce encephalitis, C57BL/6 mice were inoculated with 10 4 plaque‐forming cells of HSV‐1 by the intracranial route. Met‐RANTES (regulated upon activation, normal T cell expressed and presumably secreted) (10 μg/mouse), a CC chemokine family receptor (CCR)1 CCR5 antagonist, was given subcutaneously day before, immediately after, at days 1, 2, 3 after infection....
Herpes simplex 1 (HSV-1) causes various human clinical manifestations, ranging from simple cold sores to encephalitis. Innate immune cells recognize pathogens through Toll-like receptors (TLRs), thus initiating the response. Previously, we demonstrated that response against HSV-1 is dependent on TLR2 and TLR9 expression IFN gamma production in trigeminal ganglia (TG) of infected mice. In this work, further investigated cells, molecules, mechanisms infection control, especially those are...
Abstract Background Covid-19 has the respiratory tract as main target of infection, and patients present mainly dyspnea, pneumonia, dry cough, fever. Nevertheless, organs outside had been reported in recent studies, including gastrointestinal liver. The host innate immune system recognizes pathogen-associated molecular patterns (PAMPs) through their pattern recognition receptor (PRRs). Toll-like 7 (TLR-7) is a recognizing ssRNA (SARS-CoV-2 an ssRNA). Polymorphisms are characterized by two or...