A5026851625- Protein Structure and Dynamics
- Prion Diseases and Protein Misfolding
- Parkinson's Disease Mechanisms and Treatments
- Alzheimer's disease research and treatments
- Hemoglobinopathies and Related Disorders
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Cancer-related Molecular Pathways
- Supramolecular Self-Assembly in Materials
- Nerve injury and regeneration
- Parvovirus B19 Infection Studies
- Enzyme Structure and Function
- Neurological Disorders and Treatments
- Cancer, Hypoxia, and Metabolism
- Neuroscience and Neuropharmacology Research
- Cardiomyopathy and Myosin Studies
- Nanoparticle-Based Drug Delivery
- Mesenchymal stem cell research
- Iron Metabolism and Disorders
- Transgenic Plants and Applications
- Maternal and Perinatal Health Interventions
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Corrosion Behavior and Inhibition
- RNA Interference and Gene Delivery
- Neuroinflammation and Neurodegeneration Mechanisms
- Microbial Inactivation Methods
Universidade Federal do Rio de Janeiro
2007-2025
Fundo Brasil
2013
National Institute of Science and Technology for Structural Biology and Bioimaging
2012
University of Alabama at Birmingham
2007
University of California, San Francisco
1965-1988
University of L'Aquila
1988
Toronto General Hospital
1988
Hospital for Sick Children
1988
SickKids Foundation
1988
A misfolded form of the prion protein (PrP) is primary culprit in mammalian diseases. It has been shown that nucleic acids catalyze misfolding cellular PrP into a scrapie-like conformer. also observed interaction with nonspecific and complex can be toxic to cultured cells. No direct correlation yet drawn between changes structure toxicity due acid binding. Here we asked whether different aggregation, stability, effects are detected when nonrelated DNA sequences interact recombinant PrP....
Parkinson's disease (PD) is a progressive degenerative of the central nervous system associated with neuroinflammation and microglial cell activation. Chemokine signaling regulates neuron-glia communication triggers inflammatory profile. Herein, we identified neuronal chemokine CCL21 as major cause imbalance through CCR7 receptor pathway therapeutic implications for PD. In humans, found that transcript expression was increased in dopaminergic neurons (DANs) substantia nigra PD patients....
Refolding of a mutant form green fluorescent protein (eGFP), which only emits characteristic fluorescence when in the natively folded state, was accomplished under high hydrostatic pressure (HHP). Compression eGFP inclusion bodies (IB) at 2.40 kbar for 30 min dissociated most aggregates and reduced quantity IBs. However, 509 nm indicated that did not refold this condition. The refolding process evaluated various decompression conditions, following IB dissociation kbar. During stepwise...
Summary: In an unselected series of 500 umbilical cord blood samples from Southern Chinese infants 15 were found to have 3.1 9.8 (mean 6.5) per cent haemoglobin Bart's. Seven the re‐investigated 8–19 months after birth; in six, Bart's was no longer detected while remaining one amount had markedly decreased. Haematological studies, carried out also some parents, indicate that these are probably heterozygous for α‐thalassaemia. same stillborn infant with hydrops foetalis encountered.
α-Synuclein protein (α-syn) is a central player in Parkinson's disease (PD) and spectrum of neurodegenerative diseases collectively known as synucleinopathies. These are characterized by abnormal motor symptoms, such tremor at rest, slowness movement, rigidity posture, bradykinesia. Histopathological features PD include preferential loss dopaminergic neurons the substantia nigra formation fibrillar intraneuronal inclusions called Lewy bodies neurites, which composed primarily α-syn protein....
Amylin is a pancreatic hormone involved in the regulation of glucose metabolism and homeostasis. Restoration post-prandial basal levels human amylin diabetic individuals key controlling glycemia, glucagon, reducing insulin dose increasing satiety, among other physiologic functions. Human has high propensity to aggregate. We have addressed this issue by designing liposomal formulation. Nanoparticles multilamellar liposomes comprising were obtained with 53% encapsulation efficiency. The vitro...
A pandemia de COVID-19 impôs uma série desafios ao campo museal e a seus profissionais. Estes voltaram sua atenção para as tecnologias digitais em rede (TDR) continuarem manter contato com públicos. Seção Assistência Ensino do Museu Nacional (SAE/MN) vem, desde 2018, lançando mão suas redes sociais como ‘espaçostempos’ educativos popularização da ciência no contexto Educação Museal Online (XXXXX, 2021). Este artigo tem objetivo apresentar narrar duas ações educativas museais online que...
Alzheimer's disease (AD) is a neurodegenerative with high prevalence and morbidity, for which there are no effective therapies. Soluble oligomers of the amyloid-β peptide (AβOs) main neurotoxins involved in early synaptic dysfunction oxidative stress associated disease. The therapeutic potential bone marrow mesenchymal stem cells (MSCs) has been investigated several models neurological diseases mechanism action these based on paracrine signaling, through release trophic or neuroprotective...
The head of the P22 bacteriophage is interrupted by a unique dodecameric portal vertex that serves as conduit for entrance and exit DNA. Here, in vitro unfolding/refolding processes protein were investigated at different temperatures (1, 25, 37 °C) through use urea high hydrostatic pressure (HHP) combined with spectroscopic techniques. We have characterized an intermediate species, IU, which forms 25 °C during unfolding or refolding 2−4 M urea. IU readily amorphous aggregates, rendering...
<h3>Objective:</h3> This ongoing study aims to specify the most correlated factors with test's positivity. In addition, a decisive algorithm for genetic approach will be established, along patient's sample characterization. <h3>Background:</h3> NA <h3>Design/Methods:</h3> To that end, this is an observational retrospective and descriptive current related accuracy. Patients included have hereditary ND's suspicion, age over 18 years other etiologie excluded. <h3>Results:</h3> total, 54...
Departments of Radiology1 andBecause the presence extrauterine tumor in patients with gestational trophoblastic neoplasms (OTN) mandates use combination chemotherapy, prompt diagnosis is critical.In this prospective study we intended to determine (1) value MRI and staging GTN.(2) comparative utility vs US CT delineation pelvic GTN, (3) detection hepatic metastases (4) an imaging algorithm which would provide a thorough efficacious evaluation disease extent.-Twenty-five women aged 20 59 years...