A5023698168- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Bone and Dental Protein Studies
- RNA Interference and Gene Delivery
- Genetic Syndromes and Imprinting
- Heterotopic Ossification and Related Conditions
- Electrospun Nanofibers in Biomedical Applications
- Alkaline Phosphatase Research Studies
- Veterinary Oncology Research
- Circular RNAs in diseases
- Gut microbiota and health
- Cell Adhesion Molecules Research
- Periodontal Regeneration and Treatments
- Muscle Physiology and Disorders
- Bone Tissue Engineering Materials
- Orthopaedic implants and arthroplasty
- RNA Research and Splicing
- Wound Healing and Treatments
- Child and Adolescent Health
- Connective tissue disorders research
- Thermal Regulation in Medicine
- Bone and Joint Diseases
- Dermatologic Treatments and Research
Loughborough University
2015-2024
Cardiff and Vale University Health Board
2024
University of Birmingham
2014-2021
Abstract Extracellular vesicles (EVs) are emerging in tissue engineering as promising acellular tools, circumventing many of the limitations associated with cell‐based therapies. Epigenetic regulation through histone deacetylase (HDAC) inhibition has been shown to increase differentiation capacity. Therefore, this study aimed investigate potential augmenting osteoblast epigenetic functionality using HDAC inhibitor Trichostatin A (TSA) enhance therapeutic efficacy osteoblast‐derived EVs for...
While extracellular vesicles (EVs) continue to gain interest for therapeutic applications, their clinical translation is limited by a lack of optimal isolation methods. We sought determine how universally applied methods impact EV purity and yield. EVs were isolated ultracentrifugation (UC), polyethylene glycol precipitation, Total Exosome Isolation Reagent, an aqueous two-phase system with without repeat washes or size exclusion chromatography (SEC). EV-like particles could be detected all...
Abstract Cell culture‐conditioned medium (CCM) is a valuable source of extracellular vesicles (EVs) for basic scientific, therapeutic and diagnostic applications. culturing parameters affect the biochemical composition, release possibly function CCM‐derived EVs (CCM‐EV). The CCM‐EV task force Rigor Standardization Subcommittee International Society Extracellular Vesicles aims to identify relevant cell parameters, describe their effects based on current knowledge, recommend reporting...
The application of extracellular vesicles (EVs) as natural delivery vehicles capable enhancing tissue regeneration could represent an exciting new phase in medicine. We sought to define the capacity EVs derived from mineralising osteoblasts (MO-EVs) induce mineralisation mesenchymal stem cell (MSC) cultures and delineate underlying biochemical mechanisms involved. Strikingly, we show that addition MO-EVs MSC significantly (P < 0.05) enhanced expression alkaline phosphatase, well rate volume...
Mesenchymal stem cells isolated from rats are frequently used for tissue engineering research. However, considerable differences have been identified between rat mesenchymal and those derived humans, no defined panel of markers currently exists the isolation these cells. The aim this study was to examine effects cell sorting CD29 + /CD90 adipose bone marrow tissues on their differentiation expression cell–associated genes. Flow cytometry showed 66% 78% positivity within passage 1 cultures,...
Extracellular vesicles comprise a heterogenous population of exosomes and microvesicles that have critical roles in intercellular signalling tissue development. These complex particles been implicated as mediators the therapeutic effects stem cells via transfer an assorted cargo proteins nucleic acids, which can modulate inflammation enhance endogenous regeneration range tissues. In addition, extracellular capacity to be loaded with molecules for targeted delivery pharmaceuticals. The...
Disseminated breast cancer cells have the capacity to metastasise bone marrow and reside in a dormant state within mesenchymal stem cell niche. Research has focussed on paracrine signalling factors, such as soluble proteins, microenvironment. However, it is now clear extracellular vesicles secreted by resident into this microenvironment also play key role initiation of dormancy. Dormancy encourages reduced proliferation migration, while upregulating adhesion, thus retaining Here, MCF7 were...
Osteoblast-derived extracellular vesicles (EV) are a collection of secreted (sEVs) and matrix-bound nanoparticles that function as foci for mineral nucleation accumulation. Due to the fact sEVs can be isolated directly from culture medium mineralising osteoblasts, there is growing interest their application regenerative medicine. However, at present therapeutic advancements hindered by lack understanding precise temporal contribution matrix mineralisation. This study advances current...
Abstract Background Skeletal muscle (SkM) regenerates following injury, replacing damaged tissue with high fidelity. However, in serious injuries, non-regenerative defects leave patients loss of function, increased re-injury risk and often chronic pain. Progress treating these has been slow, advances only occurring where a comprehensive understanding regeneration gained. Tissue engineering allowed the development bioengineered models SkM which regenerate injury to support research...
Extracellular vesicles (EVs) continue to gain interest across the scientific community for diagnostic and therapeutic applications. As EV applications diversify, it is essential that researchers are aware of challenges, in particular compatibility isolation methods with downstream their clinical translation. We report outcomes first cross-comparison study looking determine parameters (EV source, starting volume, operator experience, application implementation such as cost scalability)...
Abstract Extracellular vesicles (EVs) have the potential to provide new insights into skeletal muscle (SM) physiology and pathophysiology. However, current isolation protocols often do not eliminate co‐isolated components such as lipoproteins RNA binding proteins that could confound outcomes hinder downstream clinical translation. In this study, we validated an EV protocol combined size‐exclusion chromatography (SEC) with ultrafiltration (UF) increase sample throughput, scalability purity,...
Abstract Extracellular vesicles (EVs) are emerging as a promising alternative approach to cell‐therapies. However, realize the potential of these nanoparticles new regenerative tools, healthcare materials that address current limitations systemic administration need be developed. Here, two technologies for controlling structure alginate based microgel suspensions used develop sustained local release EVs, in vitro. Microparticles formed using shearing technique compared those manufactured...
Extracellular vesicles (EVs) are lipid enveloped nanoparticles that naturally produced by cells and function in the intercellular transfer of biological material such as proteins, RNAs metabolites. They have been shown to act an autocrine paracrine manner alter functions local distant recipient cells, with a growing body evidence highlighting their wide-ranging regenerative processes stem cell maintenance, tissue repair immune modulation. EVs offer several potential advantages over therapies...
Extracellular Vesicles (EVs) are considered promising nanoscale therapeutics for bone regeneration. To date, EVs typically procured from cells on 2D tissue culture plastic, an artificial environment that limits cell growth and does not replicate in situ biochemical or biophysical conditions. This study investigated the potential of 3D printed titanium scaffolds coated with hydroxyapatite to promote therapeutic efficacy osteoblast-derived EVs. Ti6Al4V different pore sizes (500 1000 µm) shapes...
Single particle characterization has become increasingly relevant for research into extracellular vesicles, progressing from bulk analysis techniques and first-generation to comprehensive multi-parameter measurements such as nano-flow cytometry (nFCM). nFCM is a form of flow that utilizes instrumentation specifically designed nano-particle analysis, allowing thousands EVs be characterized per minute both with without the use staining techniques. High resolution side scatter (SS) detection...
Heterotopic ossification (HO) is a debilitating condition defined by the rapid formation of bone in soft tissues. What makes HO fascinating first rate at which deposited, and second fact that this structurally compositionally similar to healthy adult. If mechanisms governing are understood, they have potential be exploited for development potent osteoinductive therapies. With aim, tissue-engineered skeletal muscle was used model better understand role inflammation on phenomenon. It shown...