A5051177359- Air Quality and Health Impacts
- Inhalation and Respiratory Drug Delivery
- Nanoparticles: synthesis and applications
- Toxic Organic Pollutants Impact
- Vehicle emissions and performance
- Air Quality Monitoring and Forecasting
- Energy and Environment Impacts
- Maritime Transport Emissions and Efficiency
- Aerosol Filtration and Electrostatic Precipitation
- Occupational and environmental lung diseases
- Fiber-reinforced polymer composites
- Coagulation and Flocculation Studies
- Technical Engine Diagnostics and Monitoring
- Recycling and Waste Management Techniques
- Particle Dynamics in Fluid Flows
- Carcinogens and Genotoxicity Assessment
- Atmospheric chemistry and aerosols
- Biodiesel Production and Applications
- Polymer Foaming and Composites
- Noise Effects and Management
- Genomics, phytochemicals, and oxidative stress
- Healthcare and Environmental Waste Management
- Electrohydrodynamics and Fluid Dynamics
- Protein purification and stability
- Chemical Safety and Risk Management
Karlsruhe Institute of Technology
2012-2023
FIZ Karlsruhe – Leibniz Institute for Information Infrastructure
2007-2009
FZI Research Center for Information Technology
2002-2009
Institut für Technische und Angewandte Physik (Germany)
2008
Background Ship engine emissions are important with regard to lung and cardiovascular diseases especially in coastal regions worldwide. Known cellular responses combustion particles include oxidative stress inflammatory signalling. Objectives To provide a molecular link between the chemical physical characteristics of ship emission they elicit identify potentially harmful fractions shipping aerosols. Methods Through an air-liquid interface exposure system, we exposed human cells under...
In cell culture studies, foetal calf serum (FCS) comprising numerous different proteins is added, which might coat the surface of engineered nanomaterials (ENMs) and thus could profoundly alter their biological activities. this study, a panel industrially most relevant metal oxide nanoparticles (NPs) was screened for toxic effects in A549 lung epithelial cells RAW264.7 macrophages presence absence FCS. medium without FCS amorphous SiO2-NPs were cytotoxic NPs induced significant...
Background: Investigations on adverse biological effects of nanoparticles (NPs) in the lung by vitro studies are usually performed under submerged conditions where NPs suspended cell culture media. However, behaviour such as agglomeration and sedimentation complex suspensions is difficult to control hence deposited cellular dose often remains unknown. Moreover, responses might differ from those observed at physiological settings air–liquid interface. Results: In order avoid problems because...
A central challenge for the safe design of nanomaterials (NMs) is inherent variability NM properties, both as produced and they interact with evolve in, their surroundings. This has led to uncertainty in literature regarding whether biological toxicological effects reported NMs are related specific properties themselves, or rather presence impurities physical such agglomeration particles. Thus, there a strong need systematic evaluation synthesis processing parameters that lead potential...
Reliable and predictive in vitro assays for hazard assessments of manufactured nanomaterials (MNMs) are still limited. Specifically, exposure systems which more realistically recapitulate the physiological conditions lung needed to predict pulmonary toxicity. To this end, air-liquid interface (ALI) have been developed recent years might be better suited than conventional submerged assays. However, there is a need rigorous side-by-side comparisons results obtained with two different methods...
Elevated concentrations of particulate matter in the environmental atmosphere constitute a potential risk to human health. In vitro cell-based assays are therefore necessary evaluate toxicological inhaled emissions. this study, exposure co-culture cell model at air–liquid interface was used dose-dependent biological effects test aerosol. The CULTEX ® system expose cells an environmentally-relevant aerosol, generated from fly ash collected commercial municipal waste incinerator and...
Exposure to air pollution resulting from fossil fuel combustion has been linked multiple short-term and long term health effects. In a previous study, exposure of lung epithelial cells engine exhaust heavy oil (HFO) diesel (DF), two the main fuels used in marine engines, led an increased regulation several pathways associated with adverse cellular effects, including pro-inflammatory pathways. addition, DF was shown have wider response on regulatory levels compared HFO emissions, suggesting...
Epidemiological studies show an association between the concentration of ultrafine particles in atmosphere and rate mortality or morbidity due to respiratory cardiovascular diseases. For quantitative assessment toxicity airborne nanoparticles dose–response relationship is tested vitro test systems using bioassays cell cultures as sensor.
Abstract Background Acute exposure to elevated levels of environmental particulate matter (PM) is associated with increasing morbidity and mortality rates. These adverse health effects, e.g. culminating in respiratory cardiovascular diseases, have been demonstrated by a multitude epidemiological studies. However, the underlying mechanisms relevant for toxicity are not completely understood. Especially role particle-induced reactive oxygen species (ROS), oxidative stress inflammatory...
In recent years, the use of carbon fibers (CFs) in various sectors industry has been increasing. Despite similarity CF degradation products to other toxicologically relevant materials such as asbestos and nanotubes, a detailed toxicological evaluation this class material yet be performed. work, we exposed advanced air–liquid interface cell culture models human lung CF. To simulate different stresses applied throughout their life cycle, they were either mechanically (mCF) or...
Epidemiological studies identified air pollution as one of the prime causes for human morbidity and mortality, due to harmful effects mainly on cardiovascular respiratory systems. Damage lung leads several severe diseases such fibrosis, chronic obstructive pulmonary disease cancer. Noxious environmental aerosols are comprised a gas particulate phase representing highly complex chemical mixtures composed myriads compounds. Although some critical pollutants, foremost matter (PM), could be...
The use of nanomaterials incorporated into plastic products is increasing steadily. By using nano-scaled filling materials, thermoplastics, such as polyethylene (PE), take advantage the unique properties (NM). life cycle these so-called nanocomposites (NC) usually ends with energetic recovery. However, toxicity aerosols, which may consist released NM well combustion-generated volatile compounds, not fully understood. Within this study, model consisting a PE matrix and material (TiO2, CuO,...
The occupational exposure to particles such as crystalline quartz and its impact on the respiratory tract have been studied extensively in recent years. For hazard assessment, development of physiologically more relevant in-vitro models, i.e., air-liquid interface (ALI) cell cultures, has greatly progressed. Within this study, pulmonary culture models employing A549 differentiated THP-1 cells mono-and co-cultures were investigated. different cultures exposed α-quartz (Min-U-Sil5) with doses...
The consumption of wood fuel is markedly increasing in developing and industrialized countries. Known side effects smoke inhalation manifest proinflammatory signaling, oxidative stress, DNA damage hence increased cancer risk. In this study, the composition acute biological impact emissions state-of-the-art combustion compliances: masonry heater (MH) pellet boiler (PB) were investigated. Therefore A549 cells exposed to emission aerosols an automated air-liquid interface exposure station...
In vitro lung cell models like air-liquid interface (ALI) and 3D cultures have advanced greatly in recent years, being especially valuable for testing materials (e.g., nanomaterials, fibrous substances) when considering inhalative exposure. Within this study, we established submerged ALI culture utilizing A549 cells as mono-cultures co-cultures with differentiated THP-1 (dTHP-1), well of dTHP-1. After exposures towards α-quartz particles (Min-U-Sil5), depositions ranging from 15 to 60...
Extensive production and use of nanomaterials (NMs), such as titanium dioxide (TiO
The interactions of fly ash aerosol with lung cells were tested in vitro via exposure at the air–liquid interface or suspension medium. experimental set-up for generation, dilution and under conditions that do not affect cell viability by treatment procedure was shown to work principle. First experiments showed only low effects concerning metabolic activity release pro-inflammatory mediators NR8383 alveolar macrophages BEAS-2B epithelial cells. Exposure resuspended culture medium neither...
Abstract The applied surface dose is a key parameter for the measurement of toxic effects airborne particles by air liquid interface exposure human lung cells. Besides online deposited particle mass quartz crystal microbalance frequently other metrics such as size distribution, and agglomeration state are required. These properties their spatial distribution can be determined digital processing micrographs obtained transmission electron microscopy (TEM). Here, we report development...