A5010855100- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Carbohydrate Chemistry and Synthesis
- Allergic Rhinitis and Sensitization
- Entomological Studies and Ecology
- Galectins and Cancer Biology
- Mosquito-borne diseases and control
- Venomous Animal Envenomation and Studies
- Estrogen and related hormone effects
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Vector-borne infectious diseases
- Entomopathogenic Microorganisms in Pest Control
- Insect Resistance and Genetics
- Insect and Pesticide Research
- Immune Cell Function and Interaction
- Crystallization and Solubility Studies
- Blood groups and transfusion
- Viral Infections and Vectors
- Inhalation and Respiratory Drug Delivery
- Biochemical and Molecular Research
- X-ray Diffraction in Crystallography
- Proteoglycans and glycosaminoglycans research
- Ubiquitin and proteasome pathways
- Lanthanide and Transition Metal Complexes
University of Twente
2023-2025
Wiener Krankenanstaltenverbund
2023-2025
Universidade Federal do Rio Grande do Sul
2012-2024
Hospital de Clínicas de Porto Alegre
2017-2024
National Institute of Allergy and Infectious Diseases
2024
University of Rostock
2023-2024
Bayer (Germany)
2007-2022
Synthetic Biologics (United States)
2022
Medical University of Vienna
2022
Universidade Federal do Ceará
2021
Abstract The water footprint of a crop (WF) is common metric for assessing agricultural consumption and productivity. To provide an update methodological enhancement existing WF datasets, we apply global process-based model to quantify consumptive WFs 175 individual crops at 5 arcminute resolution over the 1990–2019 period. This simulates daily growth vertical balance considering local environmental conditions, characteristics, farm management. We partition into green (water from...
Polyunsaturated fatty acids (PUFAs) exert immunosuppressive effects, but the molecular alterations leading to T cell inhibition are not yet elucidated. Signal transduction seems involve detergent-resistant membrane domains (DRMs) acting as functional rafts within plasma bilayer with Src family protein tyrosine kinases being attached their cytoplasmic leaflet. Since DRMs include predominantly saturated acyl moieties, we investigated whether PUFAs could affect signaling by remodeling of DRMs....
Antigen-specific Abs are able to enhance or suppress immune responses depending on the receptors that they bind cells. Recent studies have shown pro- antiinflammatory effector functions of IgG also regulated through their Fc N-linked glycosylation patterns. agalactosylated (non-galactosylated) and asialylated proinflammatory induced by combination T cell-dependent (TD) protein antigens costimulation. Sialylated Abs, which immunosuppressive, Tregs produced in presence TD under tolerance...
All IgG-type antibodies are N-glycosylated in their Fc part at Asn-297. Typically, a fucose residue is attached to the first N-acetylglucosamine of these complex-type N-glycans. Antibodies lacking core fucosylation show significantly enhanced antibody-dependent cell-mediated cytotoxicity (ADCC) and an increased efficacy anti-tumor activity. In cases where clinical antibody some extent mediated by its ADCC effector function, afucosylated N-glycans could help reduce dose requirement save...
ATAD2 (ANCCA) is an epigenetic regulator and transcriptional cofactor, whose overexpression has been linked to the progress of various cancer types. Here, we report a DNA-encoded library screen leading discovery BAY-850, potent isoform selective inhibitor that specifically induces bromodomain dimerization prevents interactions with acetylated histones in vitro, as well chromatin cells. These features qualify BAY-850 chemical probe explore biology.
During the development of cancer, changes in cellular glycosylation are observed, indicating that alterations glycome occur extracellular fluids as well serum and could therefore serve tumor biomarkers. In case epithelial ovarian cancer (EOC), common markers such CA125 known to have poor specificity; therefore, better biomarkers needed. The aim this work was identify new potential glycan EOC-patients. N-Glycans were cleaved from glycoproteins 63 preoperative primary EOC-patients along with...
Cryptococcus neoformans, a pathogenic yeast, causes meningoencephalitis, especially in immunocompromised patients, leading some cases to death. Microbes biofilms can cause persistent infections, which are harder treat. Cryptococcal becoming common due the growing use of brain valves and other medical devices. Using shotgun proteomics we determine differences protein abundance between biofilm planktonic cells. Applying bioinformatic tools, also evaluated metabolic pathways involved...
Synthetic glucocorticoids (GC) are essential for the treatment of a broad range inflammatory diseases. However, their use is limited by target related adverse effects on, e.g., glucose homeostasis and bone metabolism. Starting from nonsteroidal GR ligand (4) that full agonist in reporter gene assays, we exploited key functional triggers within receptor, generating structurally diverse partial agonists. Of these, only narrow subset exhibited anti-inflammatory efficacy significantly reduced...
The aim of this report was to characterize the key physicochemical, pharmacokinetic (PK), and magnetic resonance imaging (MRI) properties gadoquatrane (BAY 1747846), a newly designed tetrameric, macrocyclic, extracellular gadolinium-based contrast agent (GBCA) with high relaxivity stability.The r1-relaxivities tetrameric at 1.41 3.0 T were determined in human plasma nuclear relaxation dispersion profiles water plasma. complex stability analyzed serum over 21 days pH 7.4 37°C compared linear...
Abstract Crop production has the largest water footprint (WF) of all economic sectors and ranks as a leading cause scarcity. Despite this, our understanding historical changes in global WFs crops remains limited. In this study, we analyse recently published dataset on green blue 175 individual 1990–2019. We explore main unit (expressed m 3 t −1 yr ) (m connect observed to various physical socio-economic drivers. find that nearly 80% reduced average (required less per tonne) crop yields...
Six novel unnatural nucleobases have been characterized that form stable base pairs in duplex DNA, relying not on hydrogen bonds, but rather interbase hydrophobic interactions. These are derivatives of the pair between 7-azaindole (7AI) and isocarbostyril (ICS). Derivatives 7AI ICS were examined increased surface area, as well polarizability. As observed with ICS, these recognized substrates by Klenow fragment Escherichia coli DNA polymerase I. The pyrrolopyrizine (PP)...
Glycosylphosphatidylinositol (GPI)-anchored proteins can deliver costimulatory signals to lymphocytes, but the exact pathway of signal transduction involved is not yet characterized. GPI-anchored are fixed cell surface solely by a phospholipid moiety and clustered in distinct membrane domains that formed an unique lipid composition requiring cholesterol. To elucidate role lipids for via proteins, we studied influence reduced cellular cholesterol content on calcium signaling CD59 CD48 Jurkat...
Effector functions and proliferation of T helper (Th) cells are influenced by cytokines in the environment.Th1 respond to a synergistic effect interleukin-12 (IL-12) interleukin-18 (IL-18) secrete interferon-gamma (IFN-γ).In contrast, Th2 interleukin-4 (IL-4) IL-4, interleukin-13 (IL-13), interleukin-5 (IL-5), interleukin-10 (IL-10).The authors were interested identifying nonpeptide inhibitors Th1 response selective for IL-12/IL-18-mediated secretion IFN-γ while leaving IL-4-mediated...
Conidial contact with an arthropod surface is the first step of fungal penetration and infection process. However, conidia Metarhizium anisopliae have associated components, like enzymes that could be involved in triggering process toxic effects not yet been well characterized. Fungi produce many also are components found bacteria animal venoms thus may considered as potential virulence factors. In this work, we report several enzymatic activities from spore protein extracts. The major...
The mangroves are among the most productive and biologically important environments. possible presence of cellulolytic enzymes microorganisms useful for biomass degradation as well taxonomic functional aspects two Brazilian were evaluated using cultivation metagenomic approaches. From a total 296 with visual differences in colony morphology growth (including bacteria, yeast filamentous fungus), 179 (60.5%) 117 (39.5%) isolated from Rio de Janeiro (RJ) Bahia (BA) samples, respectively. RJ...
Treatment of retinopathy prematurity (ROP) stage 3 plus with bevacizumab is still very controversial. We report the outcome 6 eyes 4 premature infants ROP disease treated ranibizumab monotherapy.Six threshold in zone II, were one intravitreal injection 0.03 ml ranibizumab. No prior laser or other therapy was done. Fundus examination performed to intervention and at each follow-up visit. Changes various mean vital parameters week post compared pre-intervention assessed.The gestational age...
A continuous-flow process for the synthesis of triaminophloroglucinol has been developed. The synthetic procedure is based on a sequential nitration/reduction protocol which uses phloroglucinol as an inexpensive substrate. During initial exothermic nitration step employing combination ammonium nitrate and sulfuric acid, temperature was controlled through enhanced heat transfer derived from high surface-to-volume ratio utilized capillary tubing. Clogging tubing due to precipitation...
A class of potent, nonsteroidal, selective indazole ether-based glucocorticoid receptor modulators (SGRMs) was developed for the inhaled treatment respiratory diseases. Starting from an orally available compound with demonstrated anti-inflammatory activity in rat, a soft-drug strategy implemented to ensure rapid elimination drug candidates minimize systemic GR activation. The first clinical candidate 1b (AZD5423) displayed potent inhibition lung edema rat model allergic airway inflammation...