A5084552272- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Cell Adhesion Molecules Research
- SARS-CoV-2 and COVID-19 Research
- CAR-T cell therapy research
- Glycosylation and Glycoproteins Research
- Vector-borne infectious diseases
- Viral Infections and Vectors
- Signaling Pathways in Disease
- Protease and Inhibitor Mechanisms
- Galectins and Cancer Biology
- Lipid Membrane Structure and Behavior
- Vector-Borne Animal Diseases
- Advanced Fluorescence Microscopy Techniques
- Immune Response and Inflammation
- COVID-19 Clinical Research Studies
- Immune cells in cancer
- Force Microscopy Techniques and Applications
- Cellular transport and secretion
- Toxin Mechanisms and Immunotoxins
- Immunodeficiency and Autoimmune Disorders
- Complement system in diseases
- Cytokine Signaling Pathways and Interactions
Medical University of Vienna
2016-2025
Agroécologie
2016
Université Bourgogne Franche-Comté
2016
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2016
Institut Agro Dijon
2016
University of Vienna
1998-2015
Institut National de la Recherche Agronomique
2015
Vienna General Hospital
2011
Slovak Academy of Sciences
2011
Institute of Immunology
1990-2008
Binding of ligand or antibody to certain cell-surface proteins that are anchored the membrane by glycophosphatidylinositol (GPI) can cause activation leukocytes. However, it is not known how these molecules, which lack intracellular domains, transduce signals. The GPI-linked human molecules CD59, CD55, CD48, CD24, and CD14 as well mouse Thy-1 Ly-6 were found associate with protein tyrosine kinases, key regulators cell signal transduction. A kinase associated CD48 in T cells, cells was...
CD8+ T cell immunity to SARS-CoV-2 has been implicated in COVID-19 severity and virus control. Here, we identified nonsynonymous mutations MHC-I-restricted epitopes after deep sequencing of 747 isolates. Mutant peptides exhibited diminished or abrogated MHC-I binding a cell-free vitro assay. Reduced mutant was associated with decreased proliferation, IFN-γ production cytotoxic activity cells isolated from HLA-matched patients. Single RNA ex vivo expanded, tetramer-sorted patients further...
The glycosylphosphatidylinositol (GPI)-anchored membrane protein urokinase plasminogen activator-receptor (uPA-R; CD87) is one of the key molecules involved in migration leukocytes and tumor cells. uPA bound to uPA-R provides cell proteolytic potential used for degradation extracellular matrix. also induction adhesion chemotaxis. Here, we provide a molecular explanation these uPA-R-related cellular events. By size fractionation monocyte lysate affinity isolation on its natural ligand uPA,...
Abstract Human CD38 is a cell surface molecule involved in the regulation of lymphocyte adhesion to endothelial cells. This suggests that HUVEC bear ligand(s) for on surface. By means mAb Moon-1, which specifically inhibits CD38-mediated adhesion, we have identified trans-membrane 130-kDa acting as ligand CD38. Here, report recognized by Moon-1 CD31, member Ig superfamily. conclusion based 1) cross-inhibition assays between and reference anti-CD31 mAbs; 2) sequential immunoprecipitation...
Abstract In an effort to identify immunoregulatory molecules on dendritic cells (DC), we generated and screened for mAbs capable of modulating the T cell stimulatory function DC. A particularly interesting mAb was DF272. It recognizes monocyte-derived DC, but not blood monocytes or lymphocytes, has profound immunomodulatory effects Treatment DC with intact IgG Fab DF272 enhanced their capacity. This effect accompanied by neither up-regulation costimulatory such as B7.1 (CD80), B7.2 (CD86),...
Polyunsaturated fatty acids (PUFAs) exert immunosuppressive effects, but the molecular alterations leading to T cell inhibition are not yet elucidated. Signal transduction seems involve detergent-resistant membrane domains (DRMs) acting as functional rafts within plasma bilayer with Src family protein tyrosine kinases being attached their cytoplasmic leaflet. Since DRMs include predominantly saturated acyl moieties, we investigated whether PUFAs could affect signaling by remodeling of DRMs....
Peripheral granulocytes from rheumatoid arthritis and reactive patients were recently found to express higher levels of a newly defined Ag, termed M6, in comparison healthy subjects. We present here the molecular characterization M6 Ag show that it is novel human leukocyte activation-associated cell surface glycoprotein. lymphocytes do not significantly however, appears upon 3-day PHA-activated T blasts. On monocytes, which constitutively down-regulated on day 1 but re-induced 3...
The CD31 Ag is a surface glycoprotein of 130 kDa with broad cellular distribution. We show that among peripheral human blood cells, it expressed on monocytes, granulocytes, platelets, and subpopulation lymphocytes. Activation granulocytes leads to down-regulation molecule expression. Sequence analysis quantitative measurements the relatedness other known proteins demonstrate consists six Ig constant domains each domain bears substantial similarity Fc gamma R domains. find, however, does not...
In this paper we demonstrate that granulocyte-macrophage CSF (GM-CSF) specifically induces the expression of CD1 molecules, CD1a, CD1b and CD1c, upon human monocytes. molecules appeared monocytes on day 1 stimulation with rGM-CSF, was up-regulated until 3. Monocytes cultured in presence LPS, FMLP, PMA, recombinant granulocyte-CSF, rIFN-gamma, rTNF-alpha, rIL-1 alpha, beta, rIL-6 remained negative. The induction by rGM-CSF restricted to monocytes, since no such effect observed peripheral...
CD (cluster of differentiation) Ags are cell surface molecules expressed on leukocytes and other cells relevant for the immune system. nomenclature has been universally adopted by scientific community is officially approved International Union Immunological Societies sanctioned World Health Organization. It provides a unified designation system mAbs, as well that they recognize. This was established Human Leukocyte Differentiation Antigens Workshops. In addition to defining nomenclature,...
Intracellular pathogens have developed various strategies to escape immunity enable their survival in host cells, and many bacterial preferentially reside inside macrophages, using diverse mechanisms penetrate defenses exploit high degree of metabolic diversity plasticity. Here, we characterized the interactions intracellular pathogen Chlamydia pneumoniae with polarized human macrophages. Primary monocytes were pre-differentiated granulocyte macrophage colony-stimulating factor or for 7 days...
Legionella infections are among the most important waterborne with constantly increasing numbers of cases in industrialized countries, as a result aging populations, rising immunocompromised individuals and increased need for conditioned water due to climate change. Surveillance systems is based on microbiological culture-based techniques; however, it has been shown that high percentages populations not culturable. In past two decades, relevance such viable but non-culturable (VBNC)...
Significance The nanoscale organization of ligands and receptors is critical for cellular communication yet inherently challenging to investigate. We have here devised a DNA origami-based biointerface which allows the experimenter adjust protein distances with nanometer precision as means enhance or disturb signaling while being responsive large-scale reorganization processes during cell activation. Applying this study spatial requirements T activation, we find that smallest...
Mechanical forces acting on ligand-engaged T-cell receptors (TCRs) have previously been implicated in antigen recognition, yet their magnitude, spread, and temporal behavior are still poorly defined. We here report a FRET-based sensor equipped either with TCR-reactive single chain antibody fragment or peptide-loaded MHC, the physiological TCR-ligand. The was tethered to planar glass-supported lipid bilayers (SLBs) informed most directly magnitude kinetics of TCR-imposed at molecule level....