A5088096853- Air Quality and Health Impacts
- Indoor Air Quality and Microbial Exposure
- Air Quality Monitoring and Forecasting
- Energy and Environment Impacts
- Carcinogens and Genotoxicity Assessment
- Atmospheric chemistry and aerosols
- Allergic Rhinitis and Sensitization
- Vehicle emissions and performance
- Asthma and respiratory diseases
- Toxic Organic Pollutants Impact
- Dermatology and Skin Diseases
- Effects and risks of endocrine disrupting chemicals
- Occupational exposure and asthma
- Climate Change and Health Impacts
- Maritime Transport Emissions and Efficiency
- Pharmacogenetics and Drug Metabolism
- T-cell and B-cell Immunology
- Occupational and environmental lung diseases
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Cancer Cells and Metastasis
- NF-κB Signaling Pathways
- RNA regulation and disease
- Cytokine Signaling Pathways and Interactions
- Mesenchymal stem cell research
Helmholtz Zentrum München
2011-2024
Technical University of Munich
2007-2018
CK-CARE
2015-2018
German Center for Lung Research
2015-2018
Background: Secondary organic aerosols (SOAs) formed from anthropogenic or biogenic gaseous precursors in the atmosphere substantially contribute to ambient fine particulate matter [PM ≤2.5μm aerodynamic diameter (PM2.5)] burden, which has been associated with adverse human health effects. However, there is only limited evidence on their differential toxicological impact. Objectives: We aimed discriminate effects of generated by atmospheric aging combustion soot particles (SPs) (β-pinene)...
Background Ship engine emissions are important with regard to lung and cardiovascular diseases especially in coastal regions worldwide. Known cellular responses combustion particles include oxidative stress inflammatory signalling. Objectives To provide a molecular link between the chemical physical characteristics of ship emission they elicit identify potentially harmful fractions shipping aerosols. Methods Through an air-liquid interface exposure system, we exposed human cells under...
Outdoor particulate matter (PM(10)) is associated with detrimental health effects. However, individual PM(10) exposure occurs mostly indoors. We therefore compared the toxic effects of classroom, outdoor, and residential PM(10). Indoor outdoor was collected from six schools in Munich during teaching hours homes. Particles were analyzed by scanning electron microscopy X-ray spectroscopy (EDX). Toxicity evaluated human primary keratinocytes, lung epithelial cells after metabolic activation...
Abstract Background Wood combustion emissions have been studied previously either by in vitro or vivo models using collected particles, yet most studies neglected gaseous compounds. Furthermore, a more accurate and holistic view of the toxicity aerosols can be gained with parallel direct exposure methods. Moreover, modern techniques such as air-liquid interface (ALI) exposures enable better assessment applied than, for example, previous state-of-the-art submerged cell techniques. Methods We...
The health effects of exposure to secondary organic aerosols (SOAs) are still limited. Here, we investigated and compared the toxicities soot particles (SP) coated with β-pinene SOA (SOAβPin-SP) SP naphthalene (SOANap-SP) in a human bronchial epithelial cell line (BEAS-2B) residing at air–liquid interface. SOAβPin-SP mostly contained oxygenated aliphatic compounds from photooxidation, whereas SOANap-SP significant fraction aromatic products under similar conditions. Following exposure,...
High concentrations of particulate matter (PM(10)) were measured in classrooms. This study addresses the hazard indoor particles comparison to better-studied outdoor particles. Samples taken from six schools during teaching hours. Genome-wide gene expression human BEAS-2B lung epithelial cells was analyzed and verified by quantitative PCR. Polycyclic aromatic hydrocarbons, endotoxin, cat allergen (Fel d 1) standard methods. Enhancement allergic reactivity PM(10) confirmed primary basophils....
Abstract Background Ragweed ( Ambrosia artemisiifolia ) is a strong elicitor of allergic airway inflammation with worldwide increasing prevalence. Various components ragweed pollen are thought to play role in the development responses. The aim this study was identify critical factors for allergenicity physiological model inflammation. Methods Aqueous extract, low molecular weight fraction or major allergen Amb 1 instilled intranasally on 1–11 consecutive days, and evaluated by...
Background B cells play a central role in IgE-mediated allergies. In damaged airway epithelium, they are exposed directly to aeroallergens. We aimed assess whether direct exposure of pollen constituents affects allergic sensitization. Methods from murine splenocytes and blood samples healthy donors were incubated for 8 days under Th2-like conditions with aqueous ragweed extracts (Amb-APE) or its constituents. Secreted total IgM, IgG, IgE was quantified by ELISA. Additionally, birch, grass,...
Exposure to air pollution resulting from fossil fuel combustion has been linked multiple short-term and long term health effects. In a previous study, exposure of lung epithelial cells engine exhaust heavy oil (HFO) diesel (DF), two the main fuels used in marine engines, led an increased regulation several pathways associated with adverse cellular effects, including pro-inflammatory pathways. addition, DF was shown have wider response on regulatory levels compared HFO emissions, suggesting...
Ultrafine particles (UFP) are the smallest atmospheric particulate matter linked to air pollution-related diseases. The extent which UFP's physical and chemical properties contribute its toxicity remains unclear. It is hypothesized that UFP act as carriers for chemicals drive biological responses. This study explores robust methods generating reference understand these mechanisms perform toxicological tests. Two types of combustion-related with similar elemental carbon cores but different...
Soot particles (SP) are ubiquitous components of atmospheric particulate matter and have been shown to cause various adverse health effects. In the atmosphere, freshly emitted SP can be coated by condensed low-volatility secondary organic inorganic species. addition, gas-phase oxidants may react with surface SP. Due chemical physical resemblance carbon backbone polyaromatic hydrocarbon species their potent oxidation products, we investigated biological responses BEAS-2B lung epithelial cells...
Abstract Background The function and significance of the widespread expression natural antisense transcripts (NATs) is largely unknown. ability to quantitatively assess changes in NAT for many different multiple samples would facilitate our understanding this relatively new class RNA molecules. Results Here, we demonstrate that standard analysis Affymetrix MOE430 HG-U133 GeneChips contain hundreds probe sets detect NATs. Probe carrying a "Negative Strand Matching Probes" annotation NetAffx...
The consumption of wood fuel is markedly increasing in developing and industrialized countries. Known side effects smoke inhalation manifest proinflammatory signaling, oxidative stress, DNA damage hence increased cancer risk. In this study, the composition acute biological impact emissions state-of-the-art combustion compliances: masonry heater (MH) pellet boiler (PB) were investigated. Therefore A549 cells exposed to emission aerosols an automated air-liquid interface exposure station...
The oncoprotein Cancerous Inhibitor of Protein Phosphatase 2A (CIP2A) is overexpressed in most malignancies and an obvious candidate target protein for future cancer therapies. However, the physiological importance CIP2A-mediated PP2A inhibition largely unknown. As regulates immune responses, we investigated role CIP2A normal system development during response vivo. We show that CIP2A-deficient mice (CIP2AHOZ) present a function unchallenged conditions. However when challenged with Listeria...
Pollen related allergic diseases have been increasing for decades. The reasons this increase are unknown, but environmental pollution like diesel exhaust seem to play a role. While previous studies explored the effects of pollen extracts, we studied here first time priming on native exposure using novel experimental setup. Human bronchial epithelial BEAS-2B cells were exposed birch (real life intact pollen, not extracts) at air-liquid interface (pollen-ALI). also pre-exposed in diesel-ALI...
The ubiquitous use of phthalates in various materials and the knowledge about their potential adverse effects is great concern for human health. Several studies have uncovered role carcinogenic events suggest phthalate-associated health that include pulmonary diseases. However, only limited information on toxicity available considering inhalation as route exposure. While vitro are often based submerged exposures, this study aimed to expose A549 alveolar epithelial cells at air-liquid...
Anthropogenic activities and industrialization render continuous human exposure to semi-volatile organic compounds (SVOCs) inevitable. Occupational monitoring safety implementations consider the inhalation of SVOCs as critically relevant. Due inherent properties gas/particle mixtures, risk assessment strategies should particle size-segregated SVOC association relevance released gas phase fractions. We constructed an in vitro air–liquid interface (ALI) system study distinct toxic effects...
Particularly since the wide-ranging health effects of asbestos exposure became known, great emphasis has been placed on detailed toxicity testing known but also newly developed fiber materials. Exposure to respirable pollutants like fibers can lead tissue injury causing lung diseases such as pulmonary fibrosis or cancer. In order detect toxic potential aerosols at an early stage, development suitable test systems is essential. this study, we illustrate advanced in vitro cell model closely...