A5022670010- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Lipid Membrane Structure and Behavior
- Spectroscopy Techniques in Biomedical and Chemical Research
- Cellular Mechanics and Interactions
- Mitochondrial Function and Pathology
- RNA Interference and Gene Delivery
- Cancer, Hypoxia, and Metabolism
- 3D Printing in Biomedical Research
- Advanced biosensing and bioanalysis techniques
- Cellular transport and secretion
- Metabolomics and Mass Spectrometry Studies
- bioluminescence and chemiluminescence research
- RNA Research and Splicing
- Optical Imaging and Spectroscopy Techniques
- DNA and Nucleic Acid Chemistry
- Nanoplatforms for cancer theranostics
- Photosynthetic Processes and Mechanisms
- Cancer Cells and Metastasis
- Genetic Neurodegenerative Diseases
- Photoacoustic and Ultrasonic Imaging
- DNA Repair Mechanisms
- Force Microscopy Techniques and Applications
- Genomics and Chromatin Dynamics
- Cancer Research and Treatments
Fluor (United States)
2016-2025
University of California, Irvine
2016-2025
Beckman Laser Institute and Medical Clinic
2024
Fluorescence Innovations (United States)
2010-2022
Duke University
2022
Italian Institute of Technology
2022
University of Pisa
2022
Universidad Complutense de Madrid
2022
University of Wisconsin–Madison
2022
University of Urbino
2022
We describe a label-free imaging method to monitor stem-cell metabolism that discriminates different states of stem cells as they differentiate in living tissues. In this we use intrinsic fluorescence biomarkers and the phasor approach lifetime microscopy conjunction with image segmentation, which introduce concept cell phasor. live tissues are able identify fluorophores, such collagen, retinol, retinoic acid, porphyrin, flavins, free bound NADH. have exploited detect trend metabolite...
Blood stream infection or sepsis is a major health problem worldwide, with extremely high mortality, which partly due to the inability rapidly detect and identify bacteria in early stages of infection. Here we present new technology termed 'Integrated Comprehensive Droplet Digital Detection' (IC 3D) that can selectively directly from milliliters diluted blood at single-cell sensitivity one-step, culture- amplification-free process within 1.5-4 h. The IC 3D integrates real-time, DNAzyme-based...
We report that PTEN-deficient prostate cancer cells use macropinocytosis to survive and proliferate under nutrient stress. PTEN loss increased only in the context of AMPK activation, revealing a general requirement for novel mechanism by which promotes survival In cells, albumin uptake did not require macropinocytosis, but necrotic cell debris proved specific macropinocytic cargo. Isotopic labeling confirmed macropinocytosed proteins fueled new protein synthesis cells. Supplementation with...
Biomaterial wound dressings, such as hydrogels, interact with host cells to regulate tissue repair. This study investigates how crosslinking of gelatin-based hydrogels influences immune and stromal cell behavior healing in female mice. We observe that softer, lightly crosslinked promote greater cellular infiltration result smaller scars compared stiffer, heavily hydrogels. Using single-cell RNA sequencing, we further show increase inflammation lead the formation a distinct macrophage...
Conventionally, the size, shape, and biomechanics of cartilages are determined by their voluminous extracellular matrix. By contrast, we found that multiple murine consist lipid-filled cells called lipochondrocytes. Despite resembling adipocytes, lipochondrocytes were molecularly distinct produced lipids exclusively through de novo lipogenesis. Consequently, grew uniform lipid droplets resisted systemic surges did not enlarge upon obesity. Lipochondrocytes also lacked mobilization factors,...
Summary Raster image correlation spectroscopy (RICS) is a new and novel technique for measuring molecular dynamics concentrations from fluorescence confocal images. The RICS extracts information about images of living cells taken on commercial systems. Here we develop guidelines performing the analysis an analogue laser scanning microscope. Guidelines typical instrument settings, acquisition settings detector characterization are presented. Using appropriate instrument/acquisition...
We describe a method to detect molecular complexes and measure their stoichiometry in living cells from simultaneous fluctuations of the fluorescence intensity two image channels, each detecting different kind protein. The number brightness (N&B) analysis, namely, use ratio between variance average obtain molecules, is extended cross-variance channels. apply determine containing paxillin vinculin or focal adhesion kinase (FAK) disassembling adhesions mouse embryo fibroblasts expressing...
Paxillin is an adaptor molecule involved in the assembly of focal adhesions. Using different fluorescence fluctuation approaches, we established that paxillin-EGFP dynamic on many timescales within cell, ranging from milliseconds to seconds. In cytoplasmic regions, far adhesions, paxillin uniformly distributed and freely diffusing as a monomer, determined by single-point correlation spectroscopy photon-counting histogram analysis. Near dynamics are reduced drastically, presumably due binding...
DNA damage signaling is critical for the maintenance of genome integrity and cell fate decision. Poly(ADP-ribose) polymerase 1 (PARP1) a sensor rapidly activated in dose- complexity-dependent manner playing role initial chromatin organization repair pathway choice at sites. However, our understanding cell-wide consequence its activation damaged cells still limited. Using phasor approach to fluorescence lifetime imaging microscopy fluorescence-based biosensors combination with laser...