A5034753400- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Sphingolipid Metabolism and Signaling
- Cancer, Hypoxia, and Metabolism
- Fungal and yeast genetics research
- PI3K/AKT/mTOR signaling in cancer
- Pancreatic function and diabetes
- Autophagy in Disease and Therapy
- Cellular transport and secretion
- Epigenetics and DNA Methylation
- Microtubule and mitosis dynamics
- Calcium signaling and nucleotide metabolism
- Cancer Immunotherapy and Biomarkers
- Tuberous Sclerosis Complex Research
- Polyamine Metabolism and Applications
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Cancer, Lipids, and Metabolism
- Erythrocyte Function and Pathophysiology
- Electron Spin Resonance Studies
- Metabolism, Diabetes, and Cancer
- Immunotherapy and Immune Responses
- Phagocytosis and Immune Regulation
- Biochemical and Molecular Research
- Lipid Membrane Structure and Behavior
University of California, Irvine
2013-2024
University of Southern California
2018-2023
University of Ulsan
2022
Asan Medical Center
2022
Ulsan College
2022
Seoul National University Hospital
2019
Daegu-Gyeongbuk Medical Innovation Foundation
2016
Brigham and Women's Hospital
1996
We report that PTEN-deficient prostate cancer cells use macropinocytosis to survive and proliferate under nutrient stress. PTEN loss increased only in the context of AMPK activation, revealing a general requirement for novel mechanism by which promotes survival In cells, albumin uptake did not require macropinocytosis, but necrotic cell debris proved specific macropinocytic cargo. Isotopic labeling confirmed macropinocytosed proteins fueled new protein synthesis cells. Supplementation with...
The molecular basis for changes in cytokine expression during T helper (Th) cell subset differentiation is not well understood. We have characterized transcriptional events related to gene populations of naive receptor-transgenic cells as they are driven vitro toward Th1 or Th2 phenotypes by interleukin (IL)-12 IL-4 treatment, respectively. Quantitative reverse transcriptase-polymerase chain reaction analysis transcripts indicates that interferon (IFN) gamma, IL-4, and IL-2 mRNA expressed...
The class III phosphoinositide 3-kinase (PI3K) Vps34 (also known as PIK3C3 in mammals) produces phosphatidylinositol 3-phosphate [PI(3)P] on both early and late endosome membranes to control membrane dynamics. We used Vps34-deficient cells delineate whether has additional roles endocytic trafficking. In Vps34-/- mouse embryonic fibroblasts (MEFs), transferrin recycling EEA1 localization were unaffected despite elevated Rab5-GTP levels. Strikingly, a large increase Rab7-GTP levels, an...
Oncogenic mutations drive anabolic metabolism, creating a dependency on nutrient influx through transporters, receptors, and macropinocytosis. While sphingolipids suppress tumor growth by downregulating macropinocytosis autophagy still provide cancer cells with fuel. Therapeutics that simultaneously disrupt these parallel access pathways have potential as powerful starvation agents. Here, we describe water-soluble, orally bioavailable synthetic sphingolipid, SH-BC-893, triggers transporter...
GSK5182 (4) is currently one of the lead compounds for development estrogen-related receptor gamma (ERRγ) inverse agonists. Here, we report design, synthesis, pharmacological and in vitro absorption, distribution, metabolism, excretion, toxicity (ADMET) properties a series related to 4. Starting from 4, analogs were structurally modified their ERRγ agonist activity was measured. A key pharmacophore feature this novel class ligands introduction heterocyclic group A-ring substitution core...
From yeast to humans, the cell cycle is tightly controlled by regulatory networks that regulate proliferation and can be monitored dynamic visual markers in living cells. We have observed S phase progression monitoring nuclear accumulation of FHA-containing DNA binding protein Tos4, which expressed G1/S transition. use Tos4 localization distinguish three classes replication mutants: those arrest with an apparent 1C content accumulate at restrictive temperature; 2C content, do not Tos4;...
CD47 is expressed in all human cancer cells, including head and neck cancer, initiates a signaling cascade to inhibit macrophage phagocytosis. However, the mechanism underlying overexpression has not been elucidated radioresistant cancer. The present study demonstrated that decreased Tristetraprolin (TTP) expression induced sustained of using reverse transcription-quantitative PCR western blotting, prevented phagocytosis assay HN31R cell line. Subsequently, TTP transfection, RNA...
DNA replication is generally limited to S-phase but stress can drive cells undergo synthesis outside of S-phase. Mitotic pathway known be activated deal with stress-induced chromosomal instability. There also growing evidence that residual occur in G2. We demonstrate fission yeast stimulate G2-phase not M-phase response alkylating agent MMS. Auxin-induced degradation helicase Mcm4 during G2, mitosis, inhibits post-replicative synthesis.
Upon replication fork arrest, the checkpoint kinase Cds1 is stimulated to preserve genome integrity. Robust activation of in response hydroxyurea prevents endonuclease Mus81 from cleaving stalled inappropriately. However, we find that different temperature-sensitive mcm4 mutants, affecting a subunit MCM replicative helicase. We show inhibition promotes genomic instability and allows mcm4-dg cells evade cell cycle arrest. regulation activity also contributes formation stress-induced DNA...
The elimination of residual microscopic cancer cells is important treatment. immunoediting theory describes the balance between immune system and cells. current study investigated changes in during evaluated influence cluster differentiation (CD)4 or CD8 depletion. A human squamous cell line (SNU1041) was injected lateral tongue immunocompetent mice CD4, CD8, CD11b, CD19, CD40 ligand (L) populations blood, lymph nodes spleen were using flow cytometry, serum cytokine levels a magnetic bead...
Abstract Upon replication stress, ssDNA, coated by the ssDNA-binding protein RPA, accumulates and generates a signal to activate stress response. Severe induced loss of minichromosome maintenance helicase subunit Mcm4 in temperature-sensitive Schizosaccharomyces pombe degron mutant (mcm4-dg) results formation large RPA focus that is translocated nuclear periphery. We show resection repair processes chromatin remodeler Swr1/Ino80 are involved foci its relocalization This concentrated...
Replication stress can induce DNA synthesis outside of replicative S-phase. We have previously demonstrated that fission yeast cells stimulate in G2-phase but not M-phase response to alkylating agent MMS. In this study, we show various repair pathways, including translesion and break-induced replication contribute post-replicative synthesis. Checkpoint kinases, resection proteins, multiple polymerases are also involved.
ABSTRACT From yeast to humans, the cell cycle is tightly controlled by regulatory networks that regulate proliferation and can be monitored dynamic visual markers in living cells. We have observed S phase progression monitoring nuclear accumulation of FHA-containing DNA binding protein Tos4, which expressed G1/S transition. use Tos4 localization distinguish three classes replication mutants: those arrest with an apparent 1C content accumulate at restrictive temperature; 2C content, do not...