A5011758788- Plant Reproductive Biology
- Cellular transport and secretion
- Plant Molecular Biology Research
- Cerebral Venous Sinus Thrombosis
- Aluminum toxicity and tolerance in plants and animals
- Intracerebral and Subarachnoid Hemorrhage Research
- Neurosurgical Procedures and Complications
- Neuroinflammation and Neurodegeneration Mechanisms
- Plant Micronutrient Interactions and Effects
- Infectious Diseases and Tuberculosis
- Posttraumatic Stress Disorder Research
- Silicon Effects in Agriculture
- Microtubule and mitosis dynamics
- Intracranial Aneurysms: Treatment and Complications
- Infectious Encephalopathies and Encephalitis
- Oropharyngeal Anatomy and Pathologies
- Cerebrospinal fluid and hydrocephalus
- Rheumatoid Arthritis Research and Therapies
- Parkinson's Disease Mechanisms and Treatments
- Teleoperation and Haptic Systems
- Pharmacological Effects of Natural Compounds
- Trigeminal Neuralgia and Treatments
- Chemokine receptors and signaling
- Otolaryngology and Infectious Diseases
- Arsenic contamination and mitigation
Leibniz Institute of Plant Genetics and Crop Plant Research
2019-2023
Klinikum Arnsberg
2019
University of Wisconsin–Madison
2008-2016
BOKU University
2011
Universität Hamburg
2009
University Medical Center Hamburg-Eppendorf
2009
Leipzig University
2008
Uppsala University
2007
Mayo Clinic
2007
Imperial College London
2007
The SNARE complex is a key regulator of vesicular traffic, executing membrane fusion between transport vesicles or organelles and target membranes. A functional consists four coiled-coil helical bundles, three which are supplied by Q-SNAREs another from an R-SNARE. Arabidopsis thaliana VAMP727 R-SNARE, with homologs only in seed plants. We have found that colocalizes SYP22/ VAM3, Q-SNARE, on subpopulation prevacuolar compartments/endosomes closely associated the vacuolar membrane. Genetic...
Abstract Plasma membrane proteins internalized by endocytosis and targeted for degradation are sorted into lumenal vesicles of multivesicular bodies (MVBs) the endosomal sorting complexes required transport (ESCRT) machinery. Here, we show that Arabidopsis thaliana ESCRT-related CHARGED MULTIVESICULAR BODY PROTEIN/CHROMATIN MODIFYING PROTEIN1A (CHMP1A) CHMP1B essential embryo seedling development. Double homozygous chmp1a chmp1b mutant embryos showed limited polar differentiation failed to...
Recently, an alternative route to the proteasomal protein-degradation pathway was discovered that specifically targets transmembrane proteins marked with a single ubiquitin endosomal multivesicular body (MVB) and,subsequently, vacuole (yeast) or lysosome (animals), where they are degraded by proteases. Vps23p/TSG101 is key component of ESCRT I-III machinery in yeast and animals recognizes mono-ubiquitylated sorts them into MVB. Here, we report Arabidopsis ELCH(ELC) gene encodes homolog,...
Endosomal Sorting Complex Required for Transport (ESCRT)-III proteins mediate membrane remodeling and the release of endosomal intraluminal vesicles into multivesicular bodies. Here, we show that ESCRT-III subunit paralogs CHARGED MULTIVESICULAR BODY PROTEIN1 (CHMP1A) CHMP1B are required autophagic degradation plastid in Arabidopsis thaliana. Similar to autophagy mutants, chmp1a chmp1b (chmp1) plants hyperaccumulated components, including involved division. The machinery directed bodies...
Nerve injury leads to the release of a number cytokines which have been shown play an important role in cellular activation after peripheral nerve injury. The members signal transducer and activator transcription (STAT) gene family are main mediators transduction pathway cytokines. After phosphorylation, STAT proteins transported into nucleus exhibit transcriptional activity. Following axotomy rat regenerating facial hypoglossal neurons, transient increase mRNA for JAK2, JAK3, STAT1, STAT3...
Summary Nodulin 26‐like intrinsic proteins (NIPs) play essential roles in transporting the nutrients silicon and boron seed plants, but evolutionary origin of this transport function co‐permeability to toxic arsenic remains enigmatic. Horizontal gene transfer a yet uncharacterised bacterial AqpN‐aquaporin group was starting‐point for plant NIP evolution. We combined intense sequence, phylogenetic genetic context analyses mutational approach with various assays oocytes plants resolve...
SKD1 is a core component of the mechanism that degrades plasma membrane proteins via Endosomal Sorting Complex Required for Transport (ESCRT) pathway. Its ATPase activity and endosomal recruitment are regulated by ESCRT components LIP5 IST1. How IST1 affect ESCRT-mediated trafficking development in plants not known. Here we use Arabidopsis mutants to demonstrate controls constitutive degradation formation intraluminal vesicles. Although lip5 were able polarize auxin efflux facilitators PIN2...
Direct injury of the brain is followed by inflammatory responses regulated cytokines and chemoattractants secreted from resident glia invading cells peripheral immune system. In contrast, after remote lesion central nervous system, exemplified here transection or crush facial hypoglossal nerve, locally observed activation most likely triggered damaged themselves, that is, injured neurons. The authors investigated expression monocyte chemoattractant protein MCP-1, regulation on normal T-cell...
Summary The sophisticated uptake and translocation regulation of the essential element boron (B) in plants is ensured by two transmembrane transporter families: Nodulin26‐like Intrinsic Protein ( NIP ) BOR family. Though agriculturally important crop Brassica napus highly sensitive to B deficiency, s have been suggested be responsible for efficiency this species, functional information these subfamilies extremely rare. Here, we molecularly characterized 1 family European winter‐type cv....
To evaluate the discriminative power of serial, simultaneous determinations serum neuron-specific enolase (NSE), prolactin (PRL) and creatine kinase (CK) in differentiating psychogenic non-epileptic seizures (PNES) from epileptic (ES).Prospective measurement three markers after 44 single (32 ES 12 PNES) during continuous video-EEG monitoring at seven different sampling points.Patients with had a significantly greater increase PRL 10, 20, 30 min, 1 6 h. The sensitivity for elevated NSE CK was...
The endosomal sorting complexes required for transport (ESCRT) guides transmembrane proteins to domains that bud away from the cytoplasm. ESCRT machinery consists of four complexes. 0-II are important cargo recognition and concentration via ubiquitin binding. Most membrane bending function is mediated by large multimeric ESCRT-III complex associated proteins. Here we present first in vivo proteome analysis a member which unique plant kingdom. We show with LC-MS/MS, yeast-two-hybrid (Y2H)...
Abstract Objective: To assess data on the epidemiology of nosocomial infection (N1) among neurologic intensive care patients. Design: Prospective periodic surveillance study. Setting: An 8-bed unit (ICU). Patients: All those admitted for more than 24 hours during five 3-month periods between January 1999 and March 2003. Methods: Standardized within German system. Results: Three hundred thirty-eight patients with a total 2,867 patient-days mean length stay 8.5 days were enrolled 15-month...
OBJECTIVE Although gemistocytic astrocytomas are graded as World Health Organization II astrocytomas, they behave more aggressively than other astrocytomas. Their proliferative potential is low, and it remains an intriguing question why these tumors so biologically “successful.” They show a high mutation rate of the P53 gene, cytological abnormalities, frequent perivascular mononuclear infiltrates. Microglial cells, feature this astrocytoma variant, increasing interest in context glioma...
Oilseed rape and other crops of Brassica napus have a high demand for boron (B). Boron deficiencies result in the inhibition root growth, eventually premature flower abortion. Understanding genetic mechanisms underlying abortion B-limiting conditions could provide basis to enhance B-efficiency prevent B-deficiency-related yield losses. In this study, we assessed transcriptomic responses B-deficiency diverse inflorescence tissues at multiple time points soil-grown plants that were...
Interruption of mature axons activates a cascade events in neuronal cell bodies which leads to various outcomes from functional regeneration the PNS failure any significant CNS. One factor seems play an important role molecular programs after axotomy is stearoyl Coenzyme A-desaturase-1 (SCD-1). This enzyme needed for conversion stearate into oleate. Beside its membrane synthesis, oleate could act as neurotrophic factor, involved signal transduction pathways via activation protein kinases...