A5079093287- Plant Virus Research Studies
- Plant Disease Resistance and Genetics
- Plant Pathogenic Bacteria Studies
- Genetic Mapping and Diversity in Plants and Animals
- Rice Cultivation and Yield Improvement
- Plant-Microbe Interactions and Immunity
- GABA and Rice Research
- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
- Plant and Fungal Interactions Research
- Agriculture and Rural Development Research
- Plant Pathogens and Resistance
- Plant Parasitism and Resistance
- Molecular Biology Techniques and Applications
- Legume Nitrogen Fixing Symbiosis
- Agricultural Systems and Practices
- Genetics and Plant Breeding
- Wheat and Barley Genetics and Pathology
- Biofuel production and bioconversion
- Archaeological and Historical Studies
- Plant Pathogens and Fungal Diseases
- Insect-Plant Interactions and Control
- Plant tissue culture and regeneration
- Agriculture, Land Use, Rural Development
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2022-2023
Institut de Recherche pour le Développement
2007-2023
Centre de Coopération Internationale en Recherche Agronomique pour le Développement
2006-2023
Centre Occitanie-Montpellier
2021-2023
Plant Health Institute de Montpellier
2021-2023
Institut Agro Montpellier
2022-2023
L'Institut Agro
2023
Université de Montpellier
2017-2022
Diversité, adaptation et développement des plantes
2013-2022
Agropolis International
2008-2016
More than 50 leaf rust resistance ( Lr ) genes against the fungal pathogen Puccinia triticina have been identified in wheat gene pool, and a large number of them extensively used breeding. Of genes, all are known only from their phenotype and/or map position except for Lr21 , which was cloned recently. For many years, problems molecular work (1.6 × 10 bp), highly repetitive (80%), hexaploid bread Triticum aestivum L.) genome hampered map-based cloning. Here, we report isolation Lr10 by using...
Summary In plant species with large genomes such as wheat or barley, genome organization at the level of DNA sequence is largely unknown. The largest sequences that are publicly accessible so far from Triticeae two 60 kb and 66 intervals barley. Here, we report on analysis a 211 contiguous diploid ( Triticum monococcum L.). Five putative genes were identified, which show similarity to disease resistance genes. Three five clustered in 31 gene‐enriched island while others separated cluster...
We report here evidence of the role that isoform eukaryotic translation initiation factor 4G (eIF(iso)4G) plays in naturally occurring resistance plant/virus interactions. A genetic and physical mapping approach was developed to isolate Rymv1 locus controlling high recessive Rice yellow mottle virus (RYMV) rice (Oryza sativa) variety Gigante. The mapped a 160-kb interval containing gene from eIF(iso)4G family. stable transformation resistant line with cDNA this gene, derived susceptible...
Three cultivars of Oryza sativa (IR64, Azucena, and Gigante) four O. glaberrima (Tog5681, Tog5673, CG14, SG329) were evaluated for their resistance to two isolates rice yellow mottle virus (RYMV) by enzyme-linked immunosorbent assay (ELISA) symptomatology. Cultivars Tog5681 Gigante highly resistant, no symptoms observed when either isolate was inoculated at 10 or 20 days postgermination assayed ELISA 7, 14, 22, 35, 50, 64 postinoculation. Azucena showed a partial resistance, whereas the...
The adaptation of Rice yellow mottle virus (RYMV) to recessive resistance mediated by the rymv1-2 allele has been reported as a model study emergence and evolution virulent variants. virulence factors have identified eukaryotic translation initiation factor eIF(iso)4G1 viral genome-linked protein (VPg), respectively, but molecular mechanisms involved in their interaction are still unknown. In this study, we demonstrated direct between RYMV VPg central domain rice both vitro, using...
RYMV2 is a major recessive resistance gene identified in cultivated African rice (Oryza glaberrima) which confers high to the Rice yellow mottle virus (RYMV). We mapped an approximately 30-kb interval four genes have been annotated. Sequencing of candidate region resistant Tog7291 accession revealed single mutation affecting predicted gene, as compared with RYMV-susceptible O. glaberrima CG14 reference sequence. This was found be one-base deletion leading truncated and probably nonfunctional...
Summary Rice yellow mottle virus (RYMV) causes one of the most devastating rice diseases in Africa. Management RYMV is challenging. Genetic resistance provides effective and environment‐friendly control. The recessive locus rymv2 ( OsCPR5.1 ) had been identified African Oryza glaberrima ), however, introgression into sativa ssp. japonica indica remains challenging due to crossing barriers. Here, we evaluated whether CRISPR/Cas9 genome editing two nucleoporin paralogs RYMV2 OsCPR5.2 can be...
Rice yellow mottle virus (RYMV) is one of the major diseases rice in Africa. The high resistance Oryza glaberrima Tog7291 accession involves a null allele RYMV2 gene, whose ortholog Arabidopsis, CPR5, transmembrane nucleoporin involved effector-triggered immunity. To optimize field deployment gene and improve its durability, which often weak point varietal resistance, we analyzed efficiency toward RYMV isolates representing genetic diversity molecular basis breakdown. was highly variable...
Burkholderia solanacearum race 1 isolates indigenous to the French West Indies were characterized by bacteriocin typing and two genomic fingerprinting methods: pulsed-field gel electrophoresis of DNA digested rare-cutting restriction endonucleases (RC-PFGE) PCR with primers corresponding repetitive extragenic palindromic (REP), enterobacterial intergenic consensus (ERIC), BOX elements (collectively known as rep-PCR). The survey comprised 24 reference strains 65 obtained from a field trial in...
Rice yellow mottle virus (RYMV) causes high losses to rice production in Africa. Several sources of varietal resistance are available but the emergence virulent pathotypes that able overcome one or two alleles can sometimes occur. Both spectra and viral adaptability have be taken into account develop sustainable breeding strategies against RYMV. In this study, we extended previous spectrum analyses by testing rymv1-4 rymv1-5 carried accessions Tog5438 Tog5674, respectively, isolates...
Abstract Background Rice yellow mottle virus (RYMV) is a major rice pathogen in Africa. Three resistance genes, i.e. RYMV1 , RYMV2 and RYMV3, have been previously described. encodes the translation initiation factor eIF(iso)4G1 best candidate genes for RYMV3 encode homolog of an Arabidopsis nucleoporin (CPR5) nucleotide-binding domain leucine-rich repeat containing (NLR) protein, respectively. High very uncommon Asian cultivated ( Oryza sativa ), with only two highly resistant accessions...
African rice, Oryza glaberrima, is an invaluable resource for rice cultivation and the improvement of biotic abiotic resistance properties. Since its domestication in inner Niger delta ca. 2500 years BP, has colonized a variety ecologically climatically diverse regions. However, little known about genetic basis quantitative traits adaptive variation agricultural interest this species.Using reference set 163 fully re-sequenced accessions, we report results Genome Wide Association Study...
Two doubled-haploid rice populations, IR64/Azucena and IRAT177/ Apura, were used to identify markers linked yellow mottle virus (RYMV) resistance using core restriction fragment length polymorphism (RFLP) maps. Resistance was measured by mechanical inoculation of 19-day-old seedlings followed assessment content enzyme-linked immunosorbent assay tests 15 days after inoculation. IRAT177/Apura 72 43 lines, respectively, evaluated, found be polygenic. expressed as a slower multiplication, low...
Viral diseases are a major limitation for crop production, and their control is crucial sustainable food supply. We investigated by combination of functional genetics experimental evolution the resistance rice to yellow mottle virus (RYMV), which among most devastating pathogens in Africa, mechanisms underlying extremely fast adaptation its host. found that RYMV3 gene protects against codes nucleotide-binding leucine-rich repeat domain immune receptor (NLRs) from Mla-like clade NLRs. detects...
The genetic diversity of cultivated rice in farmers’ fields remains understudied West Africa despite the importance for food security this region. In study, we genotyped samples from Burkina Faso using C6AIR SNP (Single Nucleotide Polymorphism) array (IRRI), including 27 registered cultivars and 50 collected three geographical zones western Faso. Most clustered with indica group, except seven assigned to japonica one admix. All but belonged group. other field sample, which unexpectedly Aus...
Rice yellow mottle virus (RYMV), a mechanically transmitted that causes serious damage to cultivated rice plants, is endemic Africa. Varietal selection for resistance considered be the most effective and sustainable management strategy. Standardized evaluation procedures are required identification characterization of sources. This paper describes protocol mechanical inoculation seedlings with RYMV two methods - one based on symptom severity index other detection through double antibody...