A5024057663- Redox biology and oxidative stress
- Folate and B Vitamins Research
- Glutathione Transferases and Polymorphisms
- Arsenic contamination and mitigation
- Plant Stress Responses and Tolerance
- Nitric Oxide and Endothelin Effects
- Sulfur Compounds in Biology
- Biofuel production and bioconversion
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Agriculture, Soil, Plant Science
- Plant Gene Expression Analysis
- Phytase and its Applications
- Sulfur-Based Synthesis Techniques
- Enzyme Structure and Function
- Field-Flow Fractionation Techniques
- Escherichia coli research studies
- RNA regulation and disease
- Vitamin C and Antioxidants Research
- interferon and immune responses
- Peroxisome Proliferator-Activated Receptors
- Adsorption, diffusion, and thermodynamic properties of materials
- GABA and Rice Research
- Bacterial Genetics and Biotechnology
- Nitrogen and Sulfur Effects on Brassica
- Metalloenzymes and iron-sulfur proteins
Umeå University
2024
Ghent University
2014-2019
Vrije Universiteit Brussel
2014-2019
VIB-VUB Center for Structural Biology
2014-2019
VIB-UGent Center for Plant Systems Biology
2014-2019
Bioengineering Center
2017
Indian Institute of Technology Roorkee
2013
Significance When oxygen gets incompletely reduced, reactive species (ROS) are generated. These ROS molecules can harm the building blocks of cell but also important signaling molecules. Until now, language has not been understood and a clear view is needed on how differentiates metabolic noise from that allows signaling, regulation, protection. To address this question, we focused Arabidopsis thaliana identified proteins react with hydrogen peroxide thiol amino acid cysteine, which after...
Hydrogen peroxide (H2O2) is an important messenger molecule for diverse cellular processes. H2O2 oxidizes proteinaceous cysteinyl thiols to sulfenic acid, also known as S-sulfenylation, thereby affecting the protein conformation and functionality. Although many proteins have been identified S-sulfenylation targets in plants, site-specific mapping quantification remain largely unexplored. By means of a peptide-centric chemoproteomics approach, we mapped 1,537 S-sulfenylated sites on more than...
Identifying the sulfenylation state of stressed cells is emerging as a strategic approach for detection key reactive oxygen species signaling proteins. Here, we optimized an in vivo trapping method cysteine sulfenic acids hydrogen peroxide (H2O2) plant using dimedone based DYn-2 probe. We demonstrated that specifically detects events H2O2 dose- and time-dependent way. With mass spectrometry, identified 226 sulfenylated proteins after treatment Arabidopsis cells, residing cytoplasm (123);...
Plant malate dehydrogenase (MDH) isoforms are found in different cell compartments and function key metabolic pathways. It is well known that the chloroplastic NADP-dependent MDH activities strictly redox regulated controlled by light. However, dependence of other NAD-dependent have been less studied. Here, we show vitro biochemical characterization major cytosolic isoform (cytMDH1) sensitive to H2O2 through sulfur oxidation cysteines methionines. CytMDH1 affects kinetics, secondary...
Abstract Dehydroascorbate reductase (DHAR) catalyzes the glutathione (GSH)-dependent reduction of dehydroascorbate and plays a direct role in regenerating ascorbic acid, an essential plant antioxidant vital for defense against oxidative stress. DHAR enzymes bear close structural homology to transferase (GST) superfamily contain same active site motif, but most GSTs do not exhibit activity. The presence cysteine at is catalytic functioning DHAR, as mutation this abolishes Here we present...
Pathogenic serotypes of Vibrio cholerae, transmitted through contaminated water and food, are responsible for outbreaks cholera, an acute diarrheal disease. While the cholera toxin is primary virulence factor, V. cholerae also expresses other factors, such as tripartite MakABE that secreted via bacterial flagellum. These three proteins co-expressed with two accessory proteins, MakC MakD, whose functions remain unknown. Here, we present crystal structures revealing they similar in both...