A5091400097- Toxoplasma gondii Research Studies
- Herpesvirus Infections and Treatments
- Parasitic Infections and Diagnostics
- Cytomegalovirus and herpesvirus research
- Protist diversity and phylogeny
- Ubiquitin and proteasome pathways
- HIV Research and Treatment
- Histone Deacetylase Inhibitors Research
- Polyamine Metabolism and Applications
- Viral Infections and Vectors
- Antimicrobial Peptides and Activities
- Autophagy in Disease and Therapy
- Enzyme Structure and Function
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Mosquito-borne diseases and control
- Heme Oxygenase-1 and Carbon Monoxide
- Bacteriophages and microbial interactions
- Nuclear Structure and Function
- Calcium signaling and nucleotide metabolism
Université de Lille
2011-2024
Centre Hospitalier Universitaire de Lille
2018-2024
Centre National de la Recherche Scientifique
2011-2024
Center for Infection and Immunity of Lille
2011-2024
Institut Pasteur de Lille
2012-2024
Inserm
2011-2024
Université Lille Nord de France
2012-2015
Institut Pasteur
2014
Unité de Glycobiologie Structurale et Fonctionnelle
2011
Apicomplexan parasites including Toxoplasma gondii have complex life cycles within different hosts and their infectivity relies on capacity to regulate gene expression. However, little is known about the nuclear factors that expression in these pathogens. Here, we report T. enolase TgENO2 targeted nucleus of actively replicating parasites, where it specifically binds chromatin vivo. Using a ChIP-Seq technique, provide evidence for enrichment at 5′ untranslated regions containing putative...
Toxoplasma gondii virulence depends on the expression of factors packed into specific organelles such as rhoptry and microneme. Although factor is tightly regulated, molecular mechanisms controlling their regulation remain poorly understood. ApiAP2 are a family conserved transcription (TFs) that play an important role in regulating gene apicomplexan parasites. TgAP2XI-5 able to bind transcriptionally active promoters genes expressed during S/M phase cell cycle, (rhoptries micronemes genes)....
Apicomplexan parasites have evolved efficient and distinctive strategies for intracellular replication where the timing of emergence daughter cells (budding) is a decisive element. However, molecular mechanisms that provide proper parasite budding remain unknown. Using Toxoplasma gondii as model Apicomplexan, we identified master regulator controls process. We show an ApiAP2 transcription factor, TgAP2IX-5, cell cycle events downstream centrosome duplication. TgAP2IX-5 binds to promoter...
Toxoplasmosis is a neglected parasitic disease necessitating public health control. Host cell invasion by Toxoplasma occurs at different stages of the parasite's life cycle and crucial for survival establishment infection. In tachyzoites, which are responsible acute toxoplasmosis, involves formation molecular bridge between parasite host membranes, referred to as moving junction (MJ). The MJ shaped assembly AMA1 RON2, part complex involving additional RONs. While this essential process well...
In Toxoplasma gondii, cis-acting elements present in promoter sequences of genes that are stage-specifically regulated have been described. However, the nuclear factors bind to these and regulate activities not identified. study, we performed affinity purification, followed by proteomic analysis, identify a stage-specific T. gondii. This led identification several gondii including novel factor, designated herein as TgNF3. The N-terminal domain TgNF3 shares similarities with N-terminus yeast...
Toxoplasma gondii is a eukaryotic parasite that forms latent cysts in the brain of immunocompetent individuals. The infection immune-privileged central nervous system linked to most complications. With no drug currently available eliminate infected hosts, consequences neurons' long-term are unknown. It has long been known T. specifically differentiates into form (bradyzoite) neurons, but how neuron responds remains be elucidated. We have established new vitro model resulting production...
In addition to catalyzing a central step in glycolysis, enolase assumes remarkably diverse set of secondary functions different organisms, including transcription regulation as documented for the oncogene c-Myc promoter-binding protein 1. The apicomplexan parasite Toxoplasma gondii differentially expresses two nuclear-localized, plant-like enolases: 1 (TgENO1) latent bradyzoite cyst stage and 2 (TgENO2) rapidly replicative tachyzoite stage. A 2.75 Å resolution crystal structure 1, second be...
ABSTRACT Toxoplasmosis is a critical health issue for immune-deficient individuals and the offspring of newly infected mothers. It caused by unicellular intracellular parasite called Toxoplasma gondii that found worldwide. Although efficient drugs are commonly used to treat toxoplasmosis, serious adverse events common. Therefore, new compounds with potent anti- T . activity needed provide better suited treatments. We have tested designed target specifically histone deacetylase enzymes. Among...
Pathogenesis of Toxoplasma gondii in the intermediate host is based on tachyzoite ability to divide rapidly produce significant amount daughter cells a reduce time frame. The regulation cell-cycle specific expression program therefore key their proliferation. Transcriptional has crucial role establishing this and transcription factors regulates many aspects cell cycle. We explored two ApiAP2 factors, TgAP2XII-9 TgAP2III-2, during cycle form. While TgAP2III-2 only minor impact proliferation,...
Apicomplexan parasites have unique apical rhoptry and microneme secretory organelles that are crucial for host infection, although their role in protection against Toxoplasma gondii infection is not thoroughly understood. Here, we report a novel function of the endolysosomal T. sortilin-like receptor (TgSORTLR), which mediates trafficking to functional subsequent secretion virulence factors critical induction sterile immunity parasite reinfection. We further demonstrate armadillo...
Virulence of apicomplexan parasites is based on their ability to divide rapidly produce significant biomass. The regulation cell cycle therefore key pathogenesis. Phosphorylation a crucial posttranslational modification that regulates many aspects the eukaryotic cycle. phosphatase PP1 known play major role in phosphorylation balance eukaryotes. We explored TgPP1 during tachyzoite form parasite Toxoplasma gondii. Using conditional mutant strain, we show including proper assembly daughter...
ABSTRACT Virulence of apicomplexan parasites is based on their ability to divide rapidly produce significant biomass. The regulation cell-cycle therefore key pathogenesis. Phosphorylation a crucial post-transcriptional modification that regulates many aspects the eucaryotic cell cycle. phosphatase PP1 known play major role in phosphorylation balance eukaryotes. We explored TgPP1 during cycle tachyzoite form parasite Toxoplasma gondii . Using conditional mutant strain, we show including...
ABSTRACT Toxoplasma gondii is a eukaryotic parasite that forms latent cysts in the brain of immunocompetent individuals. The infection immune-privileged central nervous system linked to most complications. With no drug currently available eliminate infected hosts, consequences neurons’ long-term are unknown. It has long been known T. specifically differentiates into form (bradyzoite) neurons, but how neuron responds remains be elucidated. We have established new vitro model resulting...