A5013076258- Respiratory viral infections research
- Neonatal Respiratory Health Research
- Immunotherapy and Immune Responses
- Viral gastroenteritis research and epidemiology
- Immune Cell Function and Interaction
- Animal Virus Infections Studies
- Pediatric health and respiratory diseases
- Influenza Virus Research Studies
- Immune Response and Inflammation
- Virus-based gene therapy research
- Congenital Diaphragmatic Hernia Studies
- Animal Disease Management and Epidemiology
- T-cell and B-cell Immunology
- Microbial infections and disease research
- Pneumonia and Respiratory Infections
- interferon and immune responses
- Gut microbiota and health
- Herpesvirus Infections and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Virology and Viral Diseases
- Tracheal and airway disorders
- Immunodeficiency and Autoimmune Disorders
- Clostridium difficile and Clostridium perfringens research
- vaccines and immunoinformatics approaches
- T-cell and Retrovirus Studies
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2019-2023
Virologie et Immunologie Moléculaires
2014-2023
Université de Versailles Saint-Quentin-en-Yvelines
2020-2023
Université Paris-Saclay
2015-2023
Centre Île-de-France - Jouy-en-Josas - Antony
2017-2019
Institut National de la Recherche Agronomique
2000-2013
Guy's Hospital
2009
King's College School
2009
Swedish University of Agricultural Sciences
1996
Mucosal vaccination induces targeted CD8 + T cell homing to inhibit mucosal tumors.
De novo protein design has been successful in expanding the natural repertoire. However, most de proteins lack biological function, presenting a major methodological challenge. In vaccinology, induction of precise antibody responses remains cornerstone for next-generation vaccines. Here, we present algorithm called TopoBuilder, with which engineered epitope-focused immunogens displaying complex structural motifs. both mice and nonhuman primates, cocktails three novo-designed induced robust...
ABSTRACT We used mRNA differential display methodology to analyze the shift of transcription profile induced by fish rhabdovirus, viral hemorrhagic septicemia virus (VHSV), in rainbow trout leukocytes. identified and characterized a new gene which is directly VHSV. This VHSV-induced ( vig-1 ) encodes 348-amino-acid protein. highly expressed during experimental disease lymphoid organs infected fish. Intramuscular injection plasmid vector expressing glycoprotein results expression, showing...
Asthma is a chronic, non-curable, multifactorial disease with increasing incidence in industrial countries. This study evaluates the direct contribution of lung microbial components allergic asthma mice. Germ-Free and Specific-Pathogen-Free mice display similar susceptibilities to House Dust Mice-induced asthma, indicating that absence bacteria confers no protection or increased risk aeroallergens. In early life, changes pattern microbiota, reciprocally modulate aeroallergen responsiveness....
Abstract Protease-activated receptor-2 (PAR2), a receptor highly expressed in the respiratory tract, can influence inflammation at mucosal surfaces. Although effects of PAR2 innate immune response to bacterial infection have been documented, knowledge its role context viral is lacking. We thus investigated influenza pathogenesis vitro and vivo. In vitro, stimulation on epithelial cells inhibited virus type A (IAV) replication through production IFN-γ. vivo, using specific agonists protected...
Abstract Dendritic cells (DC) are recognized as sentinels, which capture antigens in tissue and migrate to the lymph node, where they initiate immune responses. However, when a vaccine strain of green fluorescent protein-expressing Salmonella abortusovis (SAO) was inoculated into sheep oral mucosa, it induced accumulation myeloid non-DC subcapsular sinus paracortex draining SAO mainly found associated with these (granulocytes macrophages) but rarely DC. To analyze how bacteria reached nodes,...
Host defenses, while effecting viral clearance, contribute substantially to inflammation and disease. This double action is a substantial obstacle the development of safe effective vaccines against many agents, particularly respiratory syncytial virus (RSV). RSV common cold major cause infantile bronchiolitis worldwide. The role alphabeta T cells in RSV-driven immunopathology well studied, but little known about "unconventional" cells. During primary challenge BALB/c mice, some Vgamma7+...
Abstract Conventional dendritic cells enter lymph nodes by migrating from peripheral tissues via the lymphatic route, whereas plasmacytoid (pDC), also called IFN-producing (IPC), are described to gain blood high endothelial venules. We demonstrate here that IPC/pDC migrate in afferent of two large mammals. In sheep, injection type A CpG oligodinucleotide (ODN) induced produce I IFN. Furthermore, low-density collected at steady state produced IFN after stimulation with ODN and enveloped...
In this study, subnucleocapsid nanorings formed by the recombinant nucleoprotein (N) of respiratory syncytial virus were evaluated as a platform to anchor heterologous antigens. The ectodomain influenza A matrix protein 2 (M2e) is highly conserved and elicits protective antibodies when it linked an immunogenic carrier, making promising target develop universal vaccines. context, one or three M2e copies genetically C terminus N produce N-M2e N-3M2e chimeric nanorings. Mice immunized...
Throughout the last several decades, vaccination has been key to prevent and eradicate infectious diseases. However, many pathogens (e.g., respiratory syncytial virus [RSV], influenza, dengue, others) have resisted vaccine development efforts, largely because of failure induce potent antibody responses targeting conserved epitopes. Deep profiling human B cells often reveals neutralizing antibodies that emerge from natural infection, but these specificities are generally subdominant (i.e.,...
The homologous genes vig1 and cig5 were identified by differential display PCR as virus-induced in rainbow trout humans, respectively. These are significantly related to sequences required for the biosynthesis of metal cofactors, but their function remains unknown. In this study, it is shown that mouse homologue / was induced vesicular stomatitis virus (VSV) pseudorabies (PrV) spleen cells. Among a collection cell lines from dendritic, myeloid, lymphoid or fibroblast lineages, only dendritic...
BackgroundBronchiolitis caused by the respiratory syncytial virus (RSV) in infants less than two years old is a growing public health concern worldwide, and there currently no safe effective vaccine. A major component of RSV nucleocapsid, nucleoprotein (N), has been so far poorly explored as potential vaccine antigen, even though it target protective anti-viral T cell responses remarkably conserved between human B serotypes. We recently reported method to produce recombinant N assembling...
The development of safe and effective vaccines against both bovine human respiratory syncytial viruses (BRSV, HRSV) to be used in the presence RSV-specific maternally-derived antibodies (MDA) remains a high priority veterinary medicine. Herein, we present safety efficacy results from virulent BRSV challenge calves with MDA, which were immunized one three vaccine candidates that allow serological differentiation infected vaccinated animals (DIVA): an SH gene-deleted recombinant (ΔSHrBRSV),...
Bovine respiratory syncytial virus (BRSV) is a major cause of disease in cattle worldwide. Calves are particularly affected, even with low to moderate levels BRSV-specific maternally derived antibodies (MDA). Available BRSV vaccines have suboptimal efficacy calves MDA, and published infection models this target group lacking clinical expression. Here, we refine characterize such model.In first experiment, 2 groups 3 MDA were experimentally inoculated by inhalation aerosolized BRSV, either:...
Abstract The high incidence of lung‐damaging life‐threatening respiratory infections in infants may be related to the immaturity their immune systems. To determine whether lung features differ early life compared with those adulthood, whole as well T lymphocyte and DC responses were investigated BALB/c neonates versus adults. Higher expression GATA‐3 rapid sustained production type 2 cytokines by explants after vitro exposure anti‐CD3 was hallmark neonatal period, suggestive a Th2 bias....
Human and bovine respiratory syncytial viruses (HRSV/BRSV) are major causes of severe lower tract infections in children calves, respectively. Shared epidemiological, clinical, pathological genetic characteristics these make comparative research highly relevant. To characterise the host response against BRSV infection, bronchoalveolar lavage supernatant (BAL) from i) non-vaccinated, BRSV-infected ii) vaccinated, iii) non-infected calves was analysed by tandem mass spectrometry. Proteins were...
Respiratory syncytial virus (RSV) causes severe bronchiolitis in infants worldwide. The immunological factors responsible for RSV susceptibility are poorly understood. Here, we used the BALB/c mouse model of neonatal infection to study mechanisms leading disease upon reexposure when adults. Two major deficiencies lung innate responses were found: a poor DCs mobilization, and weak engagement IFNI pathway. administration Flt3 ligand (Flt3-L), growth factor that stimulates proliferation...