A5017855977- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- CRISPR and Genetic Engineering
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Respiratory viral infections research
- Influenza Virus Research Studies
- Nanowire Synthesis and Applications
- Blood groups and transfusion
- Viral Infectious Diseases and Gene Expression in Insects
- Viral Infections and Outbreaks Research
- Single-cell and spatial transcriptomics
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- Complement system in diseases
- Hematopoietic Stem Cell Transplantation
- Immunodeficiency and Autoimmune Disorders
- Transgenic Plants and Applications
ETH Zurich
2018-2024
Kantonsspital St. Gallen
2021
Life Science Zurich
2019
University of Zurich
2019
SUMMARY Adaptive immunity is driven by the ability of lymphocytes to undergo V(D)J recombination and generate a highly diverse set immune receptors (B cell receptors/secreted antibodies T receptors) their subsequent clonal selection expansion upon molecular recognition foreign antigens. These principles lead remarkable, unique dynamic receptor repertoires 1 . Deep sequencing provides increasing evidence for presence commonly shared (convergent) across individual organisms within one species...
Throughout the last several decades, vaccination has been key to prevent and eradicate infectious diseases. However, many pathogens (e.g., respiratory syncytial virus [RSV], influenza, dengue, others) have resisted vaccine development efforts, largely because of failure induce potent antibody responses targeting conserved epitopes. Deep profiling human B cells often reveals neutralizing antibodies that emerge from natural infection, but these specificities are generally subdominant (i.e.,...
Antibody engineering in mammalian cells offers the important advantage of expression and screening libraries their native conformation, increasing likelihood generating candidates with more favorable molecular properties. Major advances cellular enabled by CRISPR-Cas9 genome editing have made it possible to expand use biotechnological applications. Here, we describe an antibody approach where complete variable light (VL) heavy (VH) chain cassette are stably integrated into hybridoma enhanced...
Immune cell therapies based on the integration of synthetic antigen receptors comprise a powerful strategy for treatment diverse diseases, most notably T cells engineered to express chimeric (CAR) targeted cancer therapy. In addition lymphocytes, B lymphocytes may also represent valuable immune that can be therapeutic purposes such as protein replacement therapy or recombinant antibody production. this article, we report promising concept molecular design, optimization, and genomic novel...
Significance B cell clonal selection and expansion from a genetically diverse antibody repertoire guides the immune response to target antigen. It remains unclear if follow any deterministic rules or are stochastic with regards phenotypic properties such as antigen-binding, affinity, epitope specificity. We perform in-depth genotypic characterization of repertoires following immunization in mice. identify degree which is driven by binding, specificity may provide greater insight into...
Abstract Plasma cells and their secreted antibodies play a central role in the long‐term protection against chronic viral infection. However, due to experimental limitations, comprehensive description of linked genotypic, phenotypic, antibody repertoire features plasma (gene expression, clonal frequency, virus specificity, affinity) has been challenging obtain. To address this, we performed single‐cell transcriptome sequencing murine BM cell population following lymphocytic choriomeningitis...
Diverse antibody repertoires spanning multiple lymphoid organs (i.e., bone marrow, spleen, lymph nodes) form the foundation of protective humoral immunity. Changes in their composition across are a consequence B-cell selection and migration events leading to highly dynamic unique physiological landscape upon antigenic challenge (e.g., vaccination). However, what extent B cells encoding identical or similar sequences (clones) distributed how this is shaped by strength response remains largely...
Abstract Plasma cells and their secreted antibodies play a central role in the long-term protection against chronic viral infection. However, due to experimental limitations, comprehensive description of linked genotypic, phenotypic, antibody repertoire features plasma (gene expression, clonal frequency, virus specificity, affinity) has been challenging obtain. To address this, we performed single-cell transcriptome sequencing murine bone marrow cell population following lymphocytic...
ABSTRACT The capacity of humoral B cell-mediated immunity to effectively respond and protect against pathogenic infections is largely driven by the presence a diverse repertoire polyclonal antibodies in serum, which are produced plasma cells (PCs). 1,2 Recent studies have started reveal balance between deterministic mechanisms stochasticity antibody repertoires on genotypic level (i.e., clonal diversity, somatic hypermutation, germline gene usage). 3–8 However, it remains unclear if...
Abstract Diverse antibody repertoires spanning multiple lymphoid organs (e.g., bone marrow, spleen, lymph nodes) form the foundation of protective humoral immunity. Changes in their composition across are a consequence B-cell selection and migration events leading to highly dynamic unique physiological landscape upon antigenic challenge vaccination). However, what extent B cells encoding identical or similar sequences (clones) distributed how this is shaped by strength response, remains...
Abstract Immune cell therapies based on the integration of synthetic antigen receptors provide a powerful strategy for treatment diverse diseases, most notably retargeting T cells engineered to express chimeric (CAR) cancer therapy. In addition lymphocytes, B lymphocytes may also represent valuable immune that can be therapeutic purposes such as protein replacement therapy or recombinant antibody production. this article, we report promising concept molecular design, optimization and genomic...
Abstract Throughout the last decades, vaccination has been key to prevent and eradicate infectious diseases. However, many pathogens (e.g. respiratory syncytial virus (RSV), influenza, dengue others) have resisted vaccine development efforts, largely due failure induce potent antibody responses targeting conserved epitopes. Deep profiling of human B-cells often reveals neutralizing antibodies that emerge from natural infection, but these specificities are generally subdominant (i.e., present...
The Congressi Stefano Franscini (CSF) and ETH Zurich, together with support from EFIS EJI, hosted the first Synthetic Systems Immunology Conference in Ascona, Switzerland 5–8th May 2019. aim of conference was to bridge gap between synthetic systems immunology which constitute two rising progressively intertwined areas applied translational immunological research. meant provide a platform for researchers coming either gain insights into recent developments fields, exchange ideas foster...