A5031307098- Respiratory viral infections research
- Influenza Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Neonatal Respiratory Health Research
- SARS-CoV-2 and COVID-19 Research
- Bacteriophages and microbial interactions
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Congenital Diaphragmatic Hernia Studies
- Virology and Viral Diseases
- Viral Infections and Vectors
- Pneumonia and Respiratory Infections
- Bacterial Infections and Vaccines
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Vaccine Coverage and Hesitancy
- Animal Disease Management and Epidemiology
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- interferon and immune responses
- Tracheal and airway disorders
- Complement system in diseases
- Plant Virus Research Studies
- Advanced biosensing and bioanalysis techniques
- Cardiomyopathy and Myosin Studies
Hôpital Ambroise-Paré
2013-2025
Université Paris-Saclay
2016-2025
Assistance Publique – Hôpitaux de Paris
2014-2025
Inserm
2015-2025
Institut Pasteur
2007-2025
Université Paris Cité
2007-2025
Université de Versailles Saint-Quentin-en-Yvelines
2011-2024
Infection et inflammation
2014-2024
Institut thématique Immunologie, inflammation, infectiologie et microbiologie
2013-2024
Sorbonne Paris Cité
2011-2024
Infection of cells by respiratory syncytial virus induces the formation cytoplasmic inclusion bodies (IBs) where all components viral RNA polymerase complex are concentrated. However, exact organization and function these IBs remain unclear. In this study, we use conventional super-resolution imaging to dissect internal structure IBs. We observe that newly synthetized mRNA transcription anti-terminator M2-1 concentrate in IB sub-compartments, which term "IB-associated granules" (IBAGs)....
De novo protein design has been successful in expanding the natural repertoire. However, most de proteins lack biological function, presenting a major methodological challenge. In vaccinology, induction of precise antibody responses remains cornerstone for next-generation vaccines. Here, we present algorithm called TopoBuilder, with which engineered epitope-focused immunogens displaying complex structural motifs. both mice and nonhuman primates, cocktails three novo-designed induced robust...
Background Early detection and characterisation of SARS-CoV-2 variants have been continue to be essential for assessing their public health impact. In August 2023, Santé publique France implemented enhanced surveillance BA.2.86 sub-lineage JN.1 because genetic divergence from other increased prevalence. Aim To detail how combining epidemiological laboratory data sources, targeted investigations modelling enabled comprehensive JN.1. Methods Data were collected using a standardised...
ABSTRACT Following circulation of avian influenza H5 and H7 viruses in poultry, the hemagglutinin (HA) can acquire additional glycosylation sites, neuraminidase (NA) stalk becomes shorter. We investigated whether these features play a role pathogenesis infection mammalian hosts. From 1996 to 2007, H5N1 with short NA have become widespread several species. Compared long-stalk NA, short-stalk showed decreased capacity elute from red blood cells an increased virulence mice, but not chickens....
Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract illness in infants, elderly, and immunocompromised people. No vaccine or efficient antiviral treatment available against this virus. The replication transcription steps viral genome are appealing mechanisms to target for development new strategies. These activities take place within cytoplasmic inclusion bodies (IBs) that assemble during infection. Although expression both nucleoprotein (N) phosphoprotein (P)...
Abstract Respiratory syncytial virus (RSV) is a common cause of acute lower respiratory tract infection in infants, older adults and the immunocompromised. Effective directly acting antivirals are not yet available for clinical use. To address this, we screen ReFRAME drug-repurposing library consisting 12,000 small molecules against RSV. We identify 21 primary candidates including RSV F N protein inhibitors, five HSP90 four IMPDH inhibitors. select lonafarnib, licensed farnesyltransferase...
First-generation anti-SARS-CoV-2 monoclonal antibodies (mAbs) used for prophylaxis or therapeutic purposes in immunocompromised patients have been withdrawn because of the emergence resistant Omicron variants. In 2024, 2 novel mAbs, VYD222/Pemivibart and AZD3152/Sipavibart, were approved by health authorities, but their activity against contemporary JN.1 sublineages is poorly characterized.
Surveillance of the antiviral susceptibility influenza viruses in Europe revealed emergence A(H1N1) naturally resistant to anti-neuraminidase inhibitor oseltamivir (Tamiflu) [1]. Currently, are most prevalent (25%) but less Americas (16%) or Western Pacific region (4%) [2]. In Europe, prevalence varies between countries, with highest levels Norway (66.5%) and France (46.6%) [3]. These frequencies sharp contrast those observed for H1N1 during previous seasons (0 <1%) [4]–[8].
The major role of the neuraminidase (NA) protein influenza A virus is related to its sialidase activity, which disrupts interaction between envelope hemagglutinin (HA) and sialic acid receptors expressed at surface infected cells. This enzymatic activity known promote release spread progeny viral particles following their production by cells, but a potential NA in earlier steps life cycle has never been clearly demonstrated. In this study we have examined impact expression on HA-mediated...
Influenza virus sialidase has an essential role in the virus' life cycle. Two distinct groups of influenza A sialidases have been established, that differ flexibility '150-loop', providing a more open active site apo form group-1 compared to group-2 enzymes. In this study we show, through multidisciplinary approach, novel sialic acid-based derivatives can exploit structural difference and selectively inhibit activity sialidases. We also demonstrate from drug-resistant mutant viruses are...
Strong determinants of the host range influenza A viruses have been identified on polymerase complex formed by PB1, PB2, and PA subunits nucleoprotein (NP). In present study, molecular mechanisms that may involve these four core proteins contribute to restriction avian virus multiplication in human cells investigated. The efficiencies with which complexes a an isolate assemble interact viral NP cellular RNA II were compared mammalian infected cells. To this end, recombinant expressing either...
ABSTRACT The human respiratory syncytial virus (HRSV) core viral RNA polymerase comprises the large protein (L) and its cofactor, phosphoprotein (P), which associate with ribonucleoprotein complex to replicate genome and, together M2-1 protein, transcribe mRNAs. While cellular proteins have long been proposed be involved in synthesis of HRSV by associating complex, their characterization has hindered difficulty purifying from mammalian cell culture. In this study, enhanced green fluorescent...
ABSTRACT The RNA genome of respiratory syncytial virus (RSV) is constitutively encapsidated by the viral nucleoprotein N, thus forming a helical nucleocapsid. Polymerization N along genomic and antigenomic RNAs concomitant to replication requires preservation an unassembled monomeric pool. To this end, analogy with Paramyxoviridae Rhabdoviridae , it expected that phosphoprotein P acts as chaperone protein, soluble complex RNA-free form (N 0 -P complex). Here, we have engineered mutant...
Respiratory syncytial virus (RSV) RNA synthesis occurs in cytoplasmic inclusion bodies (IBs) which all the components of viral polymerase are concentrated. In this work, we show that RSV P protein recruits essential transcription factor M2-1 to IBs independently phosphorylation state M2-1. We also dephosphorylation is achieved by a complex formed between and cellular phosphatase PP1. identified PP1 binding site P, an RVxF-like motif located nearby upstream region. NMR confirmed both P-M2-1...
The current COVID-19 pandemic is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). positive-sense single-stranded RNA virus contains a single linear segment that serves as template for transcription and replication, leading to synthesis of positive negative-stranded viral (vRNA) in infected cells. Tools visualize vRNA directly cells are critical analyze replication cycle, screen therapeutic molecules, or study infections human tissue. Here, we report design,...
In autumn 2023, European vaccination campaigns predominantly administered XBB.1.5 vaccine. a multicentre study, we estimated 2023 COVID-19 vaccine effectiveness (VE) against laboratory-confirmed symptomatic infection at primary care level between September and January 2024. Using test-negative case–control design, VE in the target group for overall by time since vaccination. We included 1057 cases 4397 controls. Vaccine was 40 % (95 CI: 26–53 %) overall, 48 31–61 among those vaccinated < 6...
Geographic spread of highly pathogenic avian H5N1 influenza viruses may give rise to an pandemic. During the first months a pandemic, control measures would rely mainly on antiviral drugs, such as neuraminidase (NA) inhibitors oseltamivir and zanamivir. In this study, we compare sensitivities NAs several isolated in Asia from 1997 2005. The corresponding 50% inhibitory concentrations were determined using standard vitro NA inhibition assay. K(m) for substrate affinity inhibitor (K(i)) 2005...