A5058682606- Hepatitis C virus research
- Hepatitis B Virus Studies
- HIV/AIDS drug development and treatment
- Liver Disease Diagnosis and Treatment
- Hepatitis Viruses Studies and Epidemiology
- Liver Disease and Transplantation
- Influenza Virus Research Studies
- Viral Infections and Immunology Research
- Parvovirus B19 Infection Studies
- SARS-CoV-2 and COVID-19 Research
- Respiratory viral infections research
- Viral-associated cancers and disorders
- Pneumonia and Respiratory Infections
- Biochemical and Molecular Research
- Cytomegalovirus and herpesvirus research
- Antifungal resistance and susceptibility
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Immunotherapy and Immune Responses
- Infection Control and Ventilation
- Organ Transplantation Techniques and Outcomes
- COVID-19 and healthcare impacts
- Viral gastroenteritis research and epidemiology
- Immune responses and vaccinations
- Thermal Regulation in Medicine
Université Grenoble Alpes
2015-2025
Centre Hospitalier Universitaire de Grenoble
2014-2025
Assistance Publique – Hôpitaux de Paris
2024
Hôpital Ambroise-Paré
2024
Institut de Biologie Structurale
2016-2023
CEA Grenoble
2016-2023
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2016-2023
Centre National de la Recherche Scientifique
2013-2023
Inserm
2012-2021
Unit of Virus Host Cell Interactions
2008-2016
Ribavirin in combination with alpha 2 interferon is the consensus treatment for chronic hepatitis C. However, recent preliminary pharmacological studies have suggested that bioavailability of ribavirin displays great interindividual variability. In order to monitor serum levels during treatment, we developed and validated a quantitative assay using an approach adaptable routine hospital laboratories. The method involved solid-phase extraction on phenyl boronic acid cartridges followed by...
MDR Candida strains are emerging. Next-generation sequencing (NGS), which enables extensive and deep genome analysis, was used to investigate echinocandin azole resistance in clinical isolates. Six genes commonly involved antifungal (ERG11, ERG3, TAC1, CgPDR1, FKS1 FKS2) were analysed using NGS 40 isolates (18 albicans, 15 glabrata 7 parapsilosis). The strategy validated with known sequences. Then, 8 displaying 23 sequential collected from 10 patients receiving therapy analysed. A total of...
Background: Hepatitis B is a liver infection caused by HBV. Infected individuals who fail to control the viral develop chronic hepatitis and are at risk of developing life-threatening diseases, such as cirrhosis or cancer. Dendritic cells (DCs) play important roles in immune response against HBV but functionally impaired patients with B. The underlying mechanisms involved HBV-induced DC dysfunctions remain be elucidated. Methods: We explored modulations HBsAg exposing blood-derived cDC1s,...
Study objective To investigate the performance of a rapid RT-PCR assay to detect influenza A/B at emergency department admission. Methods This single-center prospective study recruited adult patients attending for influenza-like illness. Triage nurses performed nasopharyngeal swab samples and ran assays using dedicated device (cobas Liat, Roche Diagnostics, Meylan, France) located triage. The same sample was also analyzed in virology conventional techniques. Patients were included 24...
Abstract The prevalence of occult hepatitis B, defined by absence HBsAg and HBV DNA, ranges widely in patients with C. This may influence the treatment C severity liver disease. Sensitive specific real‐time PCR techniques are available commercially can detect more reliably low DNA levels. aim this study was to determine B virus infection using COBAS Taqman assay (Roche Diagnostics, Meylan, France) serum negative chronic evaluate its clinical consequences on pathology impact response peg‐IFNα...
For optimal antiviral therapy, the hepatitis C virus (HCV) genotype needs to be determined, as it remains a strong predictor of sustained viral response. In this study, we assessed number HCV genotyping results that could not determined using commercially available line probe assay (LiPA) (Versant 2.0 assay) in large international panel samples from 9,874 HCV-positive patients. In-house sequencing assays targeting 5' untranslated region (UTR), core region, NS3 and NS5B genome phylogenetic...
The immune control of hepatitis B virus (HBV) infection is essential for viral clearance. Therefore, restoring functional anti-HBV immunity a promising immunotherapeutic approach to treatment chronic infection. Plasmacytoid dendritic cells (pDCs) play crucial role in triggering antiviral through their ability capture and process antigens subsequently induce adaptive responses. We investigated the potential pDCs trigger cellular against HBV. used human leukocyte antigen A (HLA-A)*0201(+) pDC...
Abstract A 72-year-old immunocompromised man infected with severe acute respiratory syndrome coronavirus 2 received bamlanivimab monotherapy. Viral evolution was monitored in nasopharyngeal and blood samples by melting curve analysis of single-nucleotide polymorphisms whole-genome sequencing. Rapid emergence spike receptor binding domain mutations found, associated a compartmentalization viral populations.
Pneumocystis jirovecii is a major threat for immunocompromised patients, and clusters of pneumocystis pneumonia (PCP) have been increasingly described in transplant units during the past decade. Exploring an outbreak transmission network requires complementary spatiotemporal strain-typing approaches. We analyzed PCP demonstrated added value next-generation sequencing (NGS) multilocus sequence typing (MLST) study P. strains. Thirty-two patients were included. Among 12 solid organ 5 shared...
The automation of DNA extraction and the use commercial quantitative real-time PCR assays could help obtain more reliable results for quantification Epstein-Barr virus loads (EBV VL). This study compared two automated platforms PCRs measurement EBV VL in 10 specimens from Quality Control Molecular Diagnostics (QCMD) 200 whole-blood (WB) transplant (n = 137) nontransplant 63) patients. WB were extracted using QIAcube or MagNA Pure instrument; quantified with R-gene kit (Argene) artus RG...
ABSTRACT A national evaluation study was performed in 14 specialized laboratories with the objective of assessing their capacities to provide (i) hepatitis B virus (HBV) viral loads (VL), (ii) HBV genotypes, and(iii) identification precore/core mutants. The panel consisted 12 DNA-positive samples VLs from 2.8 9.1 log 10 copies/ml, different genotypes (A F), and 3 mutant 9 wild-type at nucleotide 1896. coefficients variation mean ranged 2.4% 10.4% Cobas Monitor assay, 1.8% 5.5% Taq Man 48,...
ABSTRACT The pretherapeutic presence of protease inhibitor (PI) resistance-associated variants (RAVs) has not been shown to be predictive triple-therapy outcomes in treatment-naive patients. However, they may influence the outcome patients with less effective pegylated interferon (pegIFN)-ribavirin (RBV) backbones. Using hepatitis C virus (HCV) population sequence analysis, we retrospectively investigated prevalence baseline nonstructural 3 (NS3) RAVs a multicenter cohort poor IFN-RBV...
Abstract Background In healthcare facilities, nosocomial transmissions of respiratory viruses are a major issue. SARS-CoV-2 is not exempt from transmission. Our goals were to describe COVID-19 cases during the first pandemic wave among patients in French university hospital and compliance with hygiene measures. Methods We conducted prospective observational study Grenoble Alpes University Hospital 01/03/2020 11/05/2020. included all hospitalised documented diagnosis. Nosocomial case was...
Since the beginning of 2014, hepatitis C virus (HCV) recombinant forms RF2k/1b have been detected in Rhône-Alpes French region 10 patients originating from Caucasus area. Circulation this particular HCV strain is very likely to be underestimated. It also prone misgenotyped when using genotyping methods based on 5' viral genome, which may lead suboptimal treatment.
The impact of basal core promoter (BCP) and precore (PC) mutants the hepatitis B virus (HBV) on liver disease severity remains controversial. aim present study was to screen BCP PC mutations in 252 HBV surface antigen (HBsAg) positive carriers France assess relationships between these severe fibrosis.Direct sequencing precore/core gene used detect A1762T/G1764A G1757A G1896A G1899A region.The prevalences A1762T/G1764A, G1757A, G1896A, were 34.1%, 38.7%, 54.9%, 29.3% (P < 0.001),...