A5000916705- Polyomavirus and related diseases
- Viral-associated cancers and disorders
- Renal Transplantation Outcomes and Treatments
- Cytomegalovirus and herpesvirus research
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 and COVID-19 Research
- Antifungal resistance and susceptibility
- Lymphoma Diagnosis and Treatment
- COVID-19 Clinical Research Studies
- Genetic Syndromes and Imprinting
- T-cell and B-cell Immunology
- Parvovirus B19 Infection Studies
- Cholinesterase and Neurodegenerative Diseases
- Pharmacological Effects and Toxicity Studies
- Cannabis and Cannabinoid Research
- Kruppel-like factors research
- Pregnancy and Medication Impact
- Complement system in diseases
- Antibiotics Pharmacokinetics and Efficacy
- Protein purification and stability
- Psoriasis: Treatment and Pathogenesis
- CNS Lymphoma Diagnosis and Treatment
- Biosimilars and Bioanalytical Methods
- SARS-CoV-2 detection and testing
- Virus-based gene therapy research
Institut de Biologie Structurale
2022-2025
Université Grenoble Alpes
2022-2025
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2022-2025
Centre National de la Recherche Scientifique
2022-2025
CEA Grenoble
2022-2025
Centre Hospitalier Universitaire de Grenoble
2017-2024
KU Leuven
2020
CHU Dijon Bourgogne
2015
SOT patients require immunosuppressors to avoid graft rejection. Therapeutic drug monitoring is insufficient find the optimal balance with immunosuppression. The evaluation of cell-mediated immunity by enzyme-linked immunospot (ELISpot) assay enumerating interferon-gamma (IFN-γ) increasingly use. ELISpot assays are performed on peripheral blood mononuclear cells (PBMC) isolated from and brought into contact specific peptides in an immunosuppressor-free environment. This study aims determine...
BackgroundBelatacept is a relevant alternative to calcineurin-inhibitors after kidney transplantation. Circulating Torque Teno Virus (TTV) DNA load correlated infectious and rejection risks post-kidney transplantation in treated patients. The aim of this study was assess the TTV profile transplant recipients converted from Belatacept explore its use as predictive biomarker.MethodsSixty-eight single-center transplanted between June-2015 December-2020 were included. Whole blood measured...
Voriconazole (VRC) overdoses are frequent and expose patients at high risk of adverse effects. This case-control study performed in hematological who benefited from VRC therapeutic drug monitoring January 2012 to December 2015 aimed identify factors overdose. Pharmacogenetic, biological, demographic parameters the time trough concentration (Cmin ) were retrospectively collected medical records. Cases (VRC overdose: defined by a Cmin ≥ 4 mg/L; n = 31) compared controls (no < using...
Abstract A 72-year-old immunocompromised man infected with severe acute respiratory syndrome coronavirus 2 received bamlanivimab monotherapy. Viral evolution was monitored in nasopharyngeal and blood samples by melting curve analysis of single-nucleotide polymorphisms whole-genome sequencing. Rapid emergence spike receptor binding domain mutations found, associated a compartmentalization viral populations.
Abstract Serological markers for Epstein–Barr virus (EBV) infection are commonly used to diagnose infectious mononucleosis and establish a serological status in pretransplant patients. This study prospectively assessed 1043 serum specimens sent the laboratory physician‐ordered EBV testing. The three markers—antiviral capsid antigen (VCA) immunoglobulin M (IgM), anti‐VCA G (IgG), anti‐Epstein–Barr nuclear (EBNA) antibodies—were tested using Elecsys Liaison immunoassays. Specimens with...
Despite the growing use of desensitization strategies, hyperimmune patients remain at high risk antibody-mediated rejection suggesting that, even when donor-specific antibodies (DSA) are effectively depleted, anti-donor specific B cells persist. We included 10 highly sensitized recipients that underwent with plasmapheresis and cell depletion prior to kidney transplantation. quantified changes in DSA (luminex), total B-cell subsets (flow cytometry), HLA (fluorospot), single-cell metabolism...
Calreticulin (CALR) mutations have recently been reported in 70–84% of JAK2V617F-negative myeloproliferative neoplasms (MPN), and this detection has become necessary to improve the diagnosis MPN. In a large single-centre cohort 298 patients suffering from Essential Thrombocythemia (ET), JAK2V617F, CALR MPL were noted 179 (60%), 56 (18.5%) 13 (4.5%) respectively. For mutations, three methods compared parallel: high-resolution melting-curve analysis (HRM), product-sizing Sanger sequencing. The...
ABSTRACT Neutralizing antibody titers and binding levels are considered correlates of protection against severe SARS-CoV-2 infection. The clinical utility serology should be reevaluated in light the emergence escape variants, as commercial antibody-binding assays have not been adapted to virus’ antigenic evolution. We compared anti-SARS-CoV-2 four quantitative serological tests based on variable ancestral spike antigens (three in-house ELISAs prototype VIDAS IgG QUANT assay) neutralization...
BK virus-associated nephropathy (PvAN) increases the risk of graft failure justifying treatment. Conversion to mammalian target rapamycin inhibitors (mTORi) and Human polyclonal immunoglobulins (IVIg) could prevent PvAN. Our retrospective study assessed efficacy mTORi associated with IVIg therapy (mTORi±IVIg group) versus standard immunosuppression reduction clear BKV DNAemia. Among forty-three kidney-transplanted patients positive DNAemia, we included twenty-six in mTORi±IVIg group reduced...
We report findings from a male fetus of 26 weeks' gestational age with severe isolated intrauterine growth restriction (IUGR). Chromosomal microarray analysis (CMA) on amniotic fluid cells revealed 1.06‐Mb duplication in 19q13.42 inherited the healthy father. This contains 34 genes including ZNF331 , gene encoding zinc‐finger protein specifically imprinted (paternally expressed) placenta. Study promoter by methylation‐specific‐multiplex ligation‐dependent probe amplification showed that...
Background: Neutralizing antibodies targeting the SARS-CoV-2 Spike protein reduce COVID-19-related risk of hospitalization, particularly in high-risk individuals. The COCOPREV-R study aimed to evaluate and compare clinical outcomes patients treated with dual monoclonal antibody therapies identify associated virological factors. Methods: retrospectively collected real-world data from receiving Bamlanivimab/Etesevimab or Casirivimab/Imdevimab (22 February 2021 15 June 2021). Results: included...
The presence of anti-HLA antibodies is an increasing challenge in kidney transplantation. Tocilizumab (TCZ), a monoclonal antibody targeting the interleukin-6 receptor (IL-6R), has been proposed to complement conventional desensitization therapy. We aimed describe TCZ plasma trough concentrations and their variability investigate link between concentration evolution antibodies. Sensitized kidney-transplant candidates treated monthly with (8 mg/kg) for were retrospectively included....