A5041964332- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- HIV Research and Treatment
- Respiratory viral infections research
- vaccines and immunoinformatics approaches
- Renal Transplantation Outcomes and Treatments
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- HIV-related health complications and treatments
- COVID-19 epidemiological studies
- CAR-T cell therapy research
- Advanced biosensing and bioanalysis techniques
- Immune Cell Function and Interaction
- Microbial infections and disease research
- Biosensors and Analytical Detection
- Long-Term Effects of COVID-19
- Viral Infections and Immunology Research
- Immunotherapy and Immune Responses
- Tracheal and airway disorders
Centre National de la Recherche Scientifique
2020-2024
Université Toulouse III - Paul Sabatier
2021-2024
Inserm
2020-2024
Université de Toulouse
2021-2024
Centre Hospitalier Universitaire de Toulouse
2021-2024
Institut de Virologie
2022-2024
Hôpital Purpan
2021-2024
Institut Toulousain des Maladies Infectieuses et Inflammatoires
2021-2022
Centre de Physiopathologie de Toulouse-Purpan
2020
BackgroundThe persistence of intact replication-competent HIV-1 proviruses is responsible for the virological rebound off treatment. The gut could be a major reservoir due to high number infected target cells.MethodsWe collected blood samples and intestinal biopsies (duodenum, ileum, colon) from 42 people with receiving effective antiretroviral therapy. We used Intact Proviral DNA Assay estimate frequency in mucosa these individuals. analyzed genetic complexity by performing single-molecule...
HIV-1 resistance testing is recommended in clinical management and next-generation sequencing (NGS) methods are now available many virology laboratories.
Abstract Background The intestinal mucosa contains many cells targeted by human immunodeficiency virus type 1 (HIV-1), and high levels of HIV-1 DNA persist in this compartment under antiretroviral therapy (ART). While CD4+ T are the best-characterized reservoir HIV-1, role long-lived macrophages persistence on ART remains controversial. Methods We collected duodenal colonic biopsies from 12 people with HIV (PWH) suppressive ART, enrolled ARNS EP61 GALT study. Total, integrated, intact...
The increasing use of monoclonal antibodies (mAbs) to treat coronavirus disease 2019 raises questions about their impact on the emergence severe acute respiratory syndrome 2 (SARS-CoV-2) mAb-resistant variants. We assessed Casirivimab-Imdevimab SARS-CoV-2 mutations associated with reduced mAb activity in treated patients.We measured nasopharyngeal (NP) viral load and sequenced haplotypes spike gene 50 patients infected delta variant using single-molecule real-time sequencing.The NP decreased...
Background: Neutralizing antibodies targeting the SARS-CoV-2 Spike protein reduce COVID-19-related risk of hospitalization, particularly in high-risk individuals. The COCOPREV-R study aimed to evaluate and compare clinical outcomes patients treated with dual monoclonal antibody therapies identify associated virological factors. Methods: retrospectively collected real-world data from receiving Bamlanivimab/Etesevimab or Casirivimab/Imdevimab (22 February 2021 15 June 2021). Results: included...
Accurate HIV-1 genome sequencing is necessary to identify drug resistance mutations (DRMs) in people with (PWH). Next-generation-sequencing (NGS) allows the detection of minor variants and now available many laboratories. Our study aimed compare two NGS approaches, a "short read" protocol using DeepChek® Whole Genome Assay on Illumina, "long pol env single-molecule real-time (SMRT) Pacific Biosciences (PacBio). We analyzed 16 plasma samples 13 cellular from PWH. whole was amplified into five...