A5043771285- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Allergic Rhinitis and Sensitization
- Hepatitis Viruses Studies and Epidemiology
- Food Allergy and Anaphylaxis Research
- COVID-19 Clinical Research Studies
- Hepatitis C virus research
- Viral gastroenteritis research and epidemiology
- Respiratory and Cough-Related Research
- Respiratory viral infections research
- Asthma and respiratory diseases
- Olfactory and Sensory Function Studies
- Liver Disease and Transplantation
- Hepatitis B Virus Studies
- Animal Virus Infections Studies
- Bacteriophages and microbial interactions
- Gastroesophageal reflux and treatments
- Eosinophilic Esophagitis
- Viral Infections and Immunology Research
- Contact Dermatitis and Allergies
- Biosensors and Analytical Detection
- Smoking Behavior and Cessation
- Infectious Diseases and Mycology
- Dermatology and Skin Diseases
- Liver Diseases and Immunity
Centre Hospitalier Universitaire de Toulouse
2017-2024
Inserm
2019-2023
Centre National de la Recherche Scientifique
2020-2023
Université Toulouse III - Paul Sabatier
2020-2023
Clinique Saint Jean Languedoc
2004-2023
Université de Toulouse
2022-2023
Institut Toulousain des Maladies Infectieuses et Inflammatoires
2021-2022
Hôpital Purpan
2020-2022
Institut de Virologie
2022
Centre de Physiopathologie de Toulouse-Purpan
2019-2020
Objective Hepatitis E virus (HEV), one of the most common agent acute hepatitis worldwide, is mainly transmitted enterically, via contaminated water for HEV genotypes 1 (HEV1) and HEV2, or by eating raw undercooked infected meat genotype 3 (HEV3) HEV4. However, little known about how ingested reaches liver its ability to replicate in intestinal cells. Design We developed human primary cultures small intestine epithelial cells explants obtained from bowel resections. The were also polarised...
Hepatitis E virus (HEV) genotype 3 is the most common linked to HEV infections in Europe and America. Three major clades (HEV-3.1, HEV-3.2, HEV-3.3) have been identified but overlaps between intra-subtype inter-subtype p-distances make subtype classification inconsistent. Reference sequences proposed facilitate communication researchers new putative subtypes recently. We used full or near full-length HEV-3 genome available Genbank database (April 2020; n = 503) distance analyses of HEV-3.1...
The emergence of the SARS-CoV-2 variants concern has greatly influenced immune correlates protection, and there are little data about antibody threshold concentrations to protect against infection with Omicron BA.1 or BA.2. We analyzed responses 259 vaccinated healthcare workers, some whom had been previously infected by SARS-CoV-2. median follow-up was 179 days (IQR: 171-182) after blood collection. detected 88 infections during period, 55 (62.5%) BA.1, 33 (37.5%) A neutralizing titer below...
Some patients with SLE or Gougerot-Sjögren's disease (GSD) receive long-term treatment hydroxychloroquine (HCQ), sometimes combined immunosuppressive therapy (IS). This study sought to assess whether HCQ that had been initiated long before the COVID-19 pandemic a protective adverse effect on risk, severity of infection immunity protection. prospective multicentre included 547 SLE, GSD, autoimmune hepatitis, primary biliary cholangitis cured viral hepatitis C divided into four groups...
To describe the sensitization profile of respiratory allergies in France, identifying factors influencing prescription allergen immunotherapy (AIT) [Transversal phase (T)], and assess treatment efficacy, tolerability, compliance satisfaction [Longitudinal (L)]. French allergists (600) pneumo-allergists were offered participation asked to recruit first 20 new patients with allergic rhinitis (AR) and/or asthma, consulting for a time allergy check-up skin prick-test (T), 5 sensitized pollens...
The present study aimed to determine whether current commercial immunoassays are adequate for detecting anti-Omicron antibodies. We analyzed the anti-SARS-CoV-2 antibody response of 23 unvaccinated individuals 1-2 months after an Omicron infection. All blood samples were tested with a live virus neutralization assay using clinical BA.1 strain and four SARS-CoV-2 immunoassays. assessed three anti-Spike (SARS-CoV-2 IgG II Quant [Abbott S], Wantaï ELISA [Wantaï], Elecsys Anti-SARS-CoV-2 S...
Studies comparing SARS-CoV-2 nasopharyngeal (NP) viral load (VL) according to virus variant and host vaccination status have yielded inconsistent results. We conducted a single center prospective study between July September 2021 at the drive-through testing of Toulouse University Hospital. compared NP VL 3775 patients infected by Delta (
The Omicron variant differs from earlier strains of SARS-CoV-2 in the way it enters host cells and grows vitro. We therefore reevaluated its diagnosis using saliva, nasopharyngeal swab (NPs), anterior nasal (ANs) specimens 202 individuals (64.9% symptomatic) tested at Toulouse University Hospital drive-through testing center. All tests were done with Thermo Fisher TaqPath COVID-19 reverse transcription-PCR (RT-PCR) kit. Overall, 92 subjects (45.5%) had one or more positive specimens. Global...
Abstract We report an analytical reversed-phase liquid-chromatographic procedure for quantifying nicotine and cotinine in urine, taking into account the presence of interfering caffeine frequently encountered such specimens. These analytes are extracted from alkalinized urine with chloroform. After evaporation chloroform, residue is dissolved methanol injected a chromatographic C18 column. Extraction recoveries averaged 80% to 97%. Chromatographic conditions were investigated obviate...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is the causal agent of COVID-19 pandemic that emerged in late 2019. outbreak variants with mutations region encoding spike protein S1 sub-unit can make them more resistant to neutralizing or monoclonal antibodies main point current monitoring. This study examines feasibility predicting variant lineage and monitoring appearance reported by sequencing only domain Pacific Bioscience Single Molecule Real-Time (PacBio SMRT). Using...
We used variant typing polymerase chain reaction to describe the evolution of severe acute respiratory syndrome coronavirus 2 Omicron sublineages between December 2021 and mid-March 2022. The selective advantage BA.2 over BA.1 is not due greater nasopharyngeal viral loads.
We investigated the seroprevalence and incidence of hepatitis E virus (HEV) infection in men who have sex with (MSM) been exposed to pre-exposure prophylaxis (PrEP) against HIV as sexual transmission HEV has suggested. A total 147 PrEP-using MSM blood donors matched for sex, age geographical area were tested anti-HEV IgG IgM. Among them, 135 followed 1 year, at end which serological tests performed retrospectively on stored samples. Laboratory data transmitted infections (STIs) viral...