A5075593785- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Hepatitis C virus research
- Hepatitis B Virus Studies
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
- Hepatitis Viruses Studies and Epidemiology
- Liver Disease Diagnosis and Treatment
- Viral Infections and Immunology Research
- Pneumonia and Respiratory Infections
- Cytomegalovirus and herpesvirus research
- HIV Research and Treatment
- Influenza Virus Research Studies
- Peripheral Neuropathies and Disorders
- Parvovirus B19 Infection Studies
- SARS-CoV-2 detection and testing
- Virus-based gene therapy research
- Herpesvirus Infections and Treatments
- Long-Term Effects of COVID-19
- Nosocomial Infections in ICU
- Infection Control and Ventilation
- Virology and Viral Diseases
- Hereditary Neurological Disorders
- Bacterial Infections and Vaccines
Hôpital Ambroise-Paré
2013-2025
Assistance Publique – Hôpitaux de Paris
2014-2025
Université de Versailles Saint-Quentin-en-Yvelines
2011-2025
Université Paris-Saclay
2020-2025
Inserm
2008-2024
Infection et inflammation
2014-2023
Institut thématique Immunologie, inflammation, infectiologie et microbiologie
2014-2022
CHU Ambroise Paré
2021-2022
Université de Rouen Normandie
2017
Normandie Université
2017
Infection of cells by respiratory syncytial virus induces the formation cytoplasmic inclusion bodies (IBs) where all components viral RNA polymerase complex are concentrated. However, exact organization and function these IBs remain unclear. In this study, we use conventional super-resolution imaging to dissect internal structure IBs. We observe that newly synthetized mRNA transcription anti-terminator M2-1 concentrate in IB sub-compartments, which term "IB-associated granules" (IBAGs)....
Hepatitis delta virus (HDV) can cause severe acute and chronic liver disease in patients infected by hepatitis B virus. Interferon alpha at high doses, although poorly efficient, is the only treatment reported to provide some benefit delta. Pegylated interferon (PEG-IFN) has not yet been evaluated. Treatment usually monitored qualitative detection of HDV-RNA serum. In this study, safety efficacy PEG-IFN were assessed delta, serum kinetics determined using quantitative RT-PCR. Fourteen with...
ABSTRACT Hepatitis D virus (HDV) is a satellite of hepatitis B (HBV) for transmission and propagation infects nearly 20 million people worldwide. The HDV genome compact circular single-stranded RNA with extensive intramolecular complementarity. Despite its different epidemiological pathological patterns, the variability geographical distribution are limited to three genotypes two subtypes that have been characterized date. Phylogenetic reconstructions based on delta antigen gene full-length...
Hepatitis delta virus is the only representative of Deltavirus genus, which consists 7 differentiated major clades. In this study, an eighth clade was identified from 3 distinct strains. genetic variability should be considered for diagnostic purposes. Clinical consequences diversity have yet to evaluated.
Background. In Western countries, the cause of 60% all Guillain-Barré syndrome (GBS) cases remains unidentified. The number unidentified peaks in winter, and these are commonly preceded by respiratory tract infection or influenza-like illness. We investigated triggering role influenza virus infection.
Background. Little is known about the epidemiology and prognostic factors of Guillain–Barré syndrome (GBS) following primary infection with cytomegalovirus (CMV-GBS).
Abstract Infection with SARS-CoV-2 variant Omicron is considered to be less severe than infection Delta, rarer occurrence of disease requiring intensive care. Little information available on comorbid factors, clinical conditions and specific viral mutational patterns associated the severity infection. In this multicenter prospective cohort study, patients consecutively admitted for COVID-19 in 20 care units France between December 7th 2021 May 1st 2022 were included. Among 259 patients, we...
A quantitative real-time PCR assay was developed to measure human cytomegalovirus (HCMV) DNA load in peripheral blood leukocytes (PBLs). The HCMV PBLs normalized by means of the quantification a cellular gene (albumin). results correlated with those pp65-antigenemia (P < 0.0001).
Currently, three genotypes of hepatitis delta virus (HDV) are described. The most common, genotype I, has a worldwide distribution; in contrast, II been found previously only Japan and Taiwan, while III is exclusively South America. Considering the high prevalence HDV Northern Siberia (Russia), restriction fragment length polymorphism (RFLP) was used to analyse from 29 infected patients living Yakutia. Of these isolates, 11 were characterized by partial nucleotide sequencing two isolates...
ABSTRACT A real-time PCR assay was developed to quantify human cytomegalovirus (HCMV) DNA in amniotic fluid (AF) samples collected from 30 pregnant women with primary HCMV infection as detected either HCMV-immunoglobulin G (IgG) seroconversion or by the presence of HCMV-specific IgG and IgM associated a low avidity. Clinical information available for each case included ultrasonographic examination fetal newborn outcome. fetuses newborns confirmed studied cases. AF were subdivided into three...
ABSTRACT Hepatitis delta virus (HDV), in association with hepatitis B virus, is responsible for severe acute and chronic hepatitis. Treatment of the infection relies on long-term administration high doses alpha interferon (IFN), treatment efficiency monitored by detection anti-HDV immunoglobulin M HDV genome serum. Like case other viral infections, quantification serum should be useful follow-up infected patients. The aims this study were to develop a quantitative assay any type evaluate...
Enteroviruses are the most frequent cause of acute meningitis and seen increasingly in sepsis-like disease fever without source paediatric population. Detection enterovirus cerebrospinal fluid (CSF) specimens by PCR is gold standard diagnostic test. Our aim was to assess a method detecting blood PCR.We did prospective, multicentre, observational study at 35 French emergency departments 16 hospitals. We recruited newborn babies (aged ≤28 days) infants >28 days ≤2 years) with source, disease,...
This study measured the impact of first wave COVID-19 pandemic (COVID-19) (March-April 2020) on incidence bloodstream infections (BSIs) at Assistance Publique - Hôpitaux de Paris (APHP), largest multisite public healthcare institution in France.The number patient admission blood cultures (BCs) collected, positive BCs, and antibiotic resistance consumption were analysed retrospectively for quarter 2020, also 2019 comparison, 25 APHP hospitals (ca. 14 000 beds).Up to a fourth patients admitted...
Hepatitis C virus (HCV) has been classified into six clades as a result of high genetic variability. In the Seine-Saint-Denis district north-east Paris, prevalence HCV-4, which usually infects populations from Africa or Middle East, is twice that recorded for whole continental France (10·2 versus 4·5%). Although pathogenicity HCV-4 remains unknown, resistance to therapy appears be similar observed HCV-1. order characterize epidemiology in sequences non-structural 5B gene (332 bp) were...
The minimum requirement for an active RNA-dependent RNA polymerase of respiratory syncytial virus (RSV) is a complex made two viral proteins, the large protein (L) and phosphoprotein (P). Here we have investigated domain on P that responsible this critical P-L interaction. By use recombinant proteins serial deletions, L binding site was mapped in C-terminal region P, just upstream N-RNA site. role molecular recognition element about 30 amino acid residues L-P interaction activity evaluated...
Abstract Background During the first COVID-19 pandemic wave, COVID-19-associated pulmonary aspergillosis (CAPA) has been reported in up to 11–28% of critically ill patients and associated with increased mortality. As new SARS-CoV-2 variants emerged, characteristics have evolved, particularly era Omicron. The purpose this study is investigate CAPA variants. Methods This a prospective multicenter observational cohort conducted France 36 participating intensive care units (ICU), between...
Background/aims Treatment of chronic delta hepatitis is long and difficult better monitoring needed. Methods In this study, virus (HDV) RNA, B surface antigen (HBsAg) (HBV) DNA were retrospectively quantified in 53 patients with e (HBeAg)-negative delta. Twenty-one had received 28 courses 3–5 MU interferon-α2b (IFN-α2b) thrice weekly for a median 12.6 months (interquartile range [IQR]: 7.3–31.6), five eight 100 mg lamivudine (LAM) daily 23.6 (IQR: 8.4–61.5) 27 untreated. The controls 54...
Respiratory syncytial virus (RSV) is the primary cause of severe respiratory infection in infants worldwide. Replication RSV genomic RNA occurs cytoplasmic inclusions generating viral ribonucleoprotein complexes (vRNPs). vRNPs then reach assembly and budding sites at plasma membrane. However, mechanisms ensuring transportation are unknown. We generated a recombinant harboring fluorescent RNPs allowing us to visualize moving living infected cells developed an automated imaging pipeline...
Abstract Human respiratory syncytial virus (RSV) is a globally prevalent negative-stranded RNA virus, which can cause life-threatening infections in young children, elderly people and immunocompromised patients. Its transcription termination factor M2-1 plays an essential role viral transcription, but the mechanisms underpinning its function are still unclear. We investigated cellular interactome of using green fluorescent protein (GFP)-trap immunoprecipitation on RSV infected cells coupled...