A5036719423- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Respiratory viral infections research
- Animal Disease Management and Epidemiology
- Cytomegalovirus and herpesvirus research
- Animal Virus Infections Studies
- RNA and protein synthesis mechanisms
- Hepatitis C virus research
- Whipple's Disease and Interleukins
- Economic Theory and Policy
- Liver Disease Diagnosis and Treatment
- Parvovirus B19 Infection Studies
- Plant Virus Research Studies
- Herpesvirus Infections and Treatments
- RNA regulation and disease
- Virus-based gene therapy research
- Hepatitis B Virus Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Medical Device Sterilization and Disinfection
- Economic theories and models
- Political Economy and Marxism
- Hepatitis Viruses Studies and Epidemiology
- Economic Theory and Institutions
- RNA modifications and cancer
- Dermatological and COVID-19 studies
Université Clermont Auvergne
2016-2025
Laboratoire Microorganismes Génome et Environnement
2015-2025
Centre National de la Recherche Scientifique
2017-2025
Centre Hospitalier Universitaire de Clermont-Ferrand
1999-2021
Laboratoire National de Référence
2018
Hospices Civils de Lyon
2014
Centre Hospitalier Universitaire de Nantes
2014
Centre Hospitalier de Versailles
2014
Centre Hospitalier Universitaire de Rennes
2014
Centre hospitalier de Tulle
2014
BackgroundHuman enteric viruses are resistant in the environment and transmitted via faecal-oral route. Viral shedding wastewater gives opportunity to track emerging pathogens study epidemiology of infectious diseases community. Aim: The aim this was monitor circulation population Clermont-Ferrand area (France) by analysis urban wastewaters. Methods: Raw treated wastewaters were collected between October 2014 2015 concentrated a two-step protocol using tangential flow ultrafiltration...
Most circulating strains of Human enterovirus 71 (EV-A71) have been classified primarily into three genogroups (A to C) on the basis genetic divergence between 1D gene, which encodes VP1 capsid protein. The aim present study was provide further insights diversity EV-A71 following recent description highly divergent isolates, in particular those from African countries, including Madagascar. We isolates by a large comparison 3,346 nucleotidic sequences collected GenBank. Analysis distances and...
We report a rapid increase in enterovirus D68 (EV-D68) infections, with 139 cases reported from eight European countries between 31 July and 14 October 2021. This upsurge is line the seasonality of EV-D68 was presumably stimulated by widespread reopening after COVID-19 lockdown. Most were identified September, but more are to be expected coming months. Reinforcement clinical awareness, diagnostic capacities surveillance urgently needed Europe.
Abstract Enterovirus D68 (EV-D68) infections are associated with severe respiratory disease and acute flaccid myelitis (AFM). The European Non-Polio Network (ENPEN) aimed to investigate the epidemiological genetic characteristics of EV-D68 its clinical impact during fall-winter season 2021–2022. From 19 countries, 58 institutes reported 10 481 (6.8%) EV-positive samples which 1004 (9.6%) were identified as (including 852 samples). Clinical data for 969 cases; 78.9% in children (0–5 years);...
On the basis of primary sequence comparisons and secondary structure predictions, picornavirus Internal ribosome entry segments (IRESes) have been divided Into three groups (entero- rhinoviruses; cardio- aphthoviruses; hepatitis A virus). Here, we describe a detailed comparison ability IRESes from each group to direct Initiation translation in vitro using single dicistronic mRNA (the only variable being IRES Inserted into region). We studied influence various parameters on capacity six...
Abstract Enteroviruses (EV) are the main etiological agents of aseptic meningitis. Diagnosis is made by detecting genome using RT‐PCR. The aim study was to evaluate impact a positive diagnosis on management infants, children, and adults. During 2005, 442 patients were admitted hospital with suspected Clinical laboratory data initial treatment recorded for all enteroviral turnaround time tests length stay analyzed. results showed that EV‐PCR detected EV in 69 (16%), 23% (16/69) About 18% CSF...
The clinical impact of enteroviruses associated with hand, foot and mouth disease (HFMD) is unknown outside Asia, the prevalence enterovirus A71 (EV-A71) in particular might be underestimated. To investigate serotypes presentations HFMD France, we conducted prospective ambulatory clinic-based surveillance children during April 2014-March 2015. Throat or buccal swabs were collected from tested for genome. Physical examinations recorded on a standardized form. An infection was detected 523...
In a one-year (October 2014-October 2015) pilot study, we assessed wastewater monitoring with sustained sampling for analysis of global enterovirus (EV) infections in an urban community. Wastewater was analysed by ultra-deep sequencing (UDS) after PCR amplification the partial VP1 capsid protein gene. The nucleotide sequence showed unprecedented diversity 48 EV types within community, which were assigned to taxonomic species A (n = 13), B 23), and C 12). During same period, 26 types, 22...
Enteroviruses are the most frequent cause of acute meningitis and seen increasingly in sepsis-like disease fever without source paediatric population. Detection enterovirus cerebrospinal fluid (CSF) specimens by PCR is gold standard diagnostic test. Our aim was to assess a method detecting blood PCR.We did prospective, multicentre, observational study at 35 French emergency departments 16 hospitals. We recruited newborn babies (aged ≤28 days) infants >28 days ≤2 years) with source, disease,...
ABSTRACT Enterovirus infections were investigated with special emphasis on performing rapid molecular identification of enterovirus serotypes responsible for aseptic meningitis directly in cerebrospinal fluid (CSF). genotyping was carried out specimens tested the diagnostic procedure, using two seminested PCR assays designed to amplify complete and partial gene sequences encoding VP1 VP4/VP2 capsid proteins, respectively. The method used identifying involved 45 patients admitted 2005....
Enterovirus 71 (EV-71) is involved in epidemics of hand, foot, and mouth disease (HFMD) has been reported to occur with severe neurological complications eastern south-east Asia. In other geographical areas, the transmission this virus poorly understood. We used large sequence datasets (of gene encoding viral protein 1, VP1) a Bayesian phylogenetic approach compare molecular epidemiology spread patterns EV-71 subgenogroups B4, B5, C1, C2, C4 Europe relative parts world. For study, European...
Enterovirus D68 (EV-D68) was detected in 93 patients from five European countries between 1 January 2019 and 15 2020, a season with expected low circulation. Patients were primarily children (n = 67, median age: 4 years), 59 required hospitalisation had severe neurologic manifestations. Phylogenetic analysis revealed two clusters the B3 subclade A2/D. This circulation of EV-D68 associated neurological manifestations stresses importance surveillance diagnostics beyond peak years.
In 2018, an upsurge in echovirus 30 (E30) infections was reported Europe. We conducted a large-scale epidemiologic and evolutionary study of 1,329 E30 strains collected 22 countries Europe during 2016-2018. Most cases affected persons 0-4 years age (29%) 25-34 (27%). Sequences were divided into 6 genetic clades (G1-G6). (53%) sequences belonged to G1, followed by G6 (23%), G2 (17%), G4 (4%), G3 (0.3%), G5 (0.2%). Each clade encompassed unique individual recombinant forms; G1 displayed >2...
We report a large-scale outbreak of hand, foot and mouth disease (HFMD) in France. As at 28 September 2021, 3,403 cases have been reported (47% higher than 2018-19). prospectively analysed 210 clinical samples; 190 (90.5%) were enterovirus-positive. Most children presented with atypical HFMD. Coxsackievirus (CV)A6 (49.5%; 94/190) was predominant; no enterovirus A71 detected. Dermatological neurological complications HFMD justify prospective syndromic virological surveillance for early...
Acute enterovirus (EV) meningitis is a major cause of hospitalization among adults and children. It caused by multiple EV genotypes assigned to 4 species (EV-A, EV-B, EV-C, EV-D).We determined viral loads in the cerebrospinal fluid (CSF) 156 patients all ages with during 5-year observational prospective study. The virus strains were genotyped, their time origin was Bayesian phylogenetic methods.The CSF ranged between 3.4 7.5 log10 copies/mL (median, 4.9 copies/mL). They higher neonates than...
Coxsackievirus A6 (CVA6) emerged as the most common enterovirus of seasonal outbreaks hand-foot-and-mouth disease (HFMD). We investigated CVA6 genetic diversity among clinical phenotypes reported in paediatric population during sentinel surveillance France between 2010 and 2018. infection was confirmed 981 children (mean age 1.52 years [IQR 1.17–2.72]) whom 564 (58%) were males. Atypical HFMD 705 (72%) children, followed by typical 214 (22%) herpangina 57 (6%) children. Throat specimens 245...
In an earlier report, different variants of echovirus 30 (E-30), enterovirus serotype, were identified during two outbreaks in 1997 and 2000. Here, the diversity E-30 was investigated over a longer period (1991–2005) variations four genomic segments determined 52 isolates involved meningitis cases, to characterize evolutionary processes underlying emergence lineages. Phylogenetic analysis VP1 sequences showed that five phylogenetic succeeded one another. When partial 3CD segment examined,...
Human enterovirus 71 (EV-71) is a cause of seasonal epidemics hand, foot and mouth disease, less common but severe neurological manifestations. Uncertainty persists regarding the circulation virus populations in several geographical areas timescale their dissemination. We determined EV-71 sequences at loci 1D (VP1 capsid protein) 3CD (non-structural proteins) 86 strains recovered Austria, France Germany performed an evolutionary genetic study extant populations. Phylogenetic analyses...