A5013587431- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Respiratory viral infections research
- Animal Virus Infections Studies
- Cytomegalovirus and herpesvirus research
- Animal Disease Management and Epidemiology
- SARS-CoV-2 and COVID-19 Research
- Whipple's Disease and Interleukins
- Parvovirus B19 Infection Studies
- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- Celiac Disease Research and Management
- Hepatitis B Virus Studies
- Vaccine Coverage and Hesitancy
- SARS-CoV-2 detection and testing
- Viral Infections and Outbreaks Research
- Career Development and Diversity
- Doctoral Education Challenges and Solutions
- Pediatric health and respiratory diseases
- Plant Virus Research Studies
- Peripheral Neuropathies and Disorders
- Viral Infections and Vectors
- Bacterial Infections and Vaccines
- COVID-19 Clinical Research Studies
- Healthcare innovation and challenges
National Institute for Public Health and the Environment
2016-2025
Public Health Institute
2025
University of Otago
2023
Erasmus University Rotterdam
2021
Erasmus MC
2021
Peter Doherty Institute
2018
Sydney Children's Hospital
2018
Amsterdam UMC Location University of Amsterdam
2007-2018
Truman Medical Center
2018
Children's Mercy Hospital
2018
Human parechoviruses (HPeVs) are members of the family Picornaviridae and classified into 3 known serotypes: HPeV1, HPeV2, recently identified HPeV3. HPeV1 HPeV2 infections most commonly associated with mild respiratory or gastrointestinal symptoms occasionally severe disease conditions, such as flaccid paralysis encephalitis. HPeV3 infection has been transient neonatal until now only reported in Japan Canada.Culture isolates considered to be enterovirus on basis cell culture but that were...
Enteroviruses (EVs) belong to the family Picornaviridae and are a well-known cause of neonatal sepsis viral meningitis. Human parechoviruses (HPeVs) type 1 2, previously named echovirus 22 23, have been associated with mild gastrointestinal or respiratory symptoms in young children. Six HPeV genotypes currently known, which HPeV3 is meningitis.Cerebrospinal fluid samples from children aged <5 years tested by EV-specific polymerase chain reaction (PCR) during 2004-2006 were selected (N= 761)....
ABSTRACT Infections with human parechoviruses (HPeVs) are prevalent in young children and have been associated mild gastroenteritis and, less frequently, meningitis neonatal sepsis. To investigate the involvement of these viruses respiratory disease, a highly sensitive nested PCR was used to screen large archive specimens, collected between January December 2007. Respiratory samples had previously tested for eight viruses, including syncytial virus adenovirus, by PCR. HPeV detected 34 3,844...
Human parechoviruses (HPeV) are widespread pathogens belonging to the Picornavirus family. Six genotypes, which have predominantly been isolated from children, known. Data on prevalence of HPeV genotypes generally based cell culture, may underestimate certain strains that difficult grow. We studied 1,824 stool samples 1,379 children (<5 years old) sent Academic Medical Center, Amsterdam, The Netherlands, between 2004 and 2006. Samples were screened using specific human enterovirus (HEV)...
Abstract Rapid detection of infection is essential for stopping the spread severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The Roche SD Biosensor rapid antigen test SARS-CoV-2 was evaluated in a nonhospitalized symptomatic population. We rapid-tested sample onsite and compared results with those from reverse transcription PCR virus culture. analyzed date onset symptoms using data clinical questionnaire. Overall sensitivity 84.9% (95% CI 79.1–89.4) specificity 99.5% 98.7–99.8)....
Abstract Objective To assess the diagnostic test accuracy of two rapid antigen tests in asymptomatic and presymptomatic close contacts people with SARS-CoV-2 infection on day 5 after exposure. Design Prospective cross sectional study. Setting Four public health service covid-19 sites Netherlands. Participants 4274 consecutively included (identified through test-and-trace programme or contact tracing app) aged 16 years older for when requesting a test. Main outcome measures Sensitivity,...
We report a rapid increase in enterovirus D68 (EV-D68) infections, with 139 cases reported from eight European countries between 31 July and 14 October 2021. This upsurge is line the seasonality of EV-D68 was presumably stimulated by widespread reopening after COVID-19 lockdown. Most were identified September, but more are to be expected coming months. Reinforcement clinical awareness, diagnostic capacities surveillance urgently needed Europe.
Abstract Background Enteroviruses (EV) cause yearly outbreaks with severe infections, particularly in young children. This study investigates EV circulation, age-distribution, and clinical presentations Europe from 2018-2023. Methods Aggregated data were requested ECDC National Focal Points for Surveillance European Non-Polio Enterovirus Network. Data included detection month, specimen type, age-group, presentation the ten most commonly reported types per year. Findings Twenty-eight...
We identified a novel human parechovirus (HPeV) type (K251176-02) from neonate with fever. Analysis of the complete genome showed K251176-02 to be new HPeV genotype. Since could not neutralized antibodies against known serotypes 1-3, it should classified as fourth serotype.
Binary file ES_Abstracts_Final_ECDC.txt matches
Immunodeficient individuals who excrete vaccine-derived polioviruses threaten polio eradication. Antivirals address this threat.In a randomized, blinded, placebo-controlled study, adults were challenged with monovalent oral poliovirus type 1 vaccine (mOPV1) and subsequently treated capsid inhibitor pocapavir or placebo. The time to virus negativity in stool was determined.A total of 144 participants enrolled; 98% became infected upon OPV challenge. Pocapavir-treated subjects (n = 93) cleared...
Human parechoviruses (HPeVs) are highly prevalent pathogens among very young children. Although originally classified into two serologically distinct types, HPeV1 and -2, recent analyses of variants collected worldwide have revealed the existence 12 further types genetically by sequence comparisons complete genome sequences or capsid (VP1) gene. To investigate nature HPeV evolution, its population dynamics recombination breakpoints, this study generated 18 full-length genomic most commonly...
Human parechoviruses (HPeVs) are highly prevalent RNA viruses classified in the family Picornaviridae. Several antigenically distinct types circulate human populations worldwide, whilst recombination additionally contributes to genetic heterogeneity of virus. To investigate factors influencing likelihood and compare its dynamics among types, 154 variants collected from four widely geographically separated referral centres (UK, The Netherlands, Thailand Brazil) were typed by VP3/VP1...
Human parechoviruses (HPeVs) are picornaviruses frequently infecting humans. While HPeV1 is associated with mild disease, HPeV3 the cause of neonatal sepsis and meningitis. To test whether in vitro replication kinetics could be related to pathogenicity, strains isolated from patients were cultured on cell lines gastrointestinal, respiratory neural origin, measured by real-time PCR. No relationship was found between clinical symptoms strains. In contrast, showed faster cells there a higher...
Significance Acute hemorrhagic conjunctivitis (AHC) is a painful and highly contagious infection of the eye, with reported incidence rates up to 48%. No drugs or vaccines are available for treatment prevention AHC. Coxsackievirus A24 variant (CV-A24v) main etiological agent AHC, being responsible >10 million AHC cases worldwide during last decades. We have identified CV-A24v protein receptor determined high-resolution structure virus–receptor complex. Furthermore, we found that an...
Polioviruses (PVs) are members of the genus Enterovirus In Netherlands, exclusion PV circulation is based on clinical enterovirus (EV) surveillance (CEVS) EV-positive cases and routine environmental EV (EEVS) conducted sewage samples collected in region Netherlands where vaccination coverage low due to religious reasons. We compared EEVS data those CEVS gain insight into relevance for poliovirus nonpolio surveillance. Following polio outbreak Syria, was performed at primary refugee center...
Enterovirus D68 (EV-D68) was detected in 93 patients from five European countries between 1 January 2019 and 15 2020, a season with expected low circulation. Patients were primarily children (n = 67, median age: 4 years), 59 required hospitalisation had severe neurologic manifestations. Phylogenetic analysis revealed two clusters the B3 subclade A2/D. This circulation of EV-D68 associated neurological manifestations stresses importance surveillance diagnostics beyond peak years.
In 2018, an upsurge in echovirus 30 (E30) infections was reported Europe. We conducted a large-scale epidemiologic and evolutionary study of 1,329 E30 strains collected 22 countries Europe during 2016-2018. Most cases affected persons 0-4 years age (29%) 25-34 (27%). Sequences were divided into 6 genetic clades (G1-G6). (53%) sequences belonged to G1, followed by G6 (23%), G2 (17%), G4 (4%), G3 (0.3%), G5 (0.2%). Each clade encompassed unique individual recombinant forms; G1 displayed >2...
Human parechoviruses (HPeVs) and enteroviruses (EVs) belong to the family Picornaviridae. EVs are known cause a wide range of disease such as meningitis, encephalitis, sepsis. HPeV1 2 have been associated with mild gastrointestinal or respiratory symptoms in young children. HPeV3 is more severe neonatal infection. Little about epidemiology pathology HPeV4-6 children.We evaluated clinical children an HPeV 4, 5, 6 The patients positive stool samples were selected available plasma cerebrospinal...