A5019386827- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Respiratory viral infections research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Bacillus and Francisella bacterial research
- Poxvirus research and outbreaks
- Animal Disease Management and Epidemiology
- Cytomegalovirus and herpesvirus research
- Influenza Virus Research Studies
- Immune Response and Inflammation
- Herpesvirus Infections and Treatments
- Parvovirus B19 Infection Studies
- Reproductive tract infections research
- Bioactive natural compounds
- Microbial infections and disease research
- Blood groups and transfusion
- COVID-19 epidemiological studies
- RNA and protein synthesis mechanisms
- Natural product bioactivities and synthesis
- SARS-CoV-2 detection and testing
- Pneumonia and Respiratory Infections
- Phytochemistry and Biological Activities
- Bacterial Infections and Vaccines
Public Health Service of Amsterdam
2022-2025
University of Amsterdam
2010-2023
Erasmus University Rotterdam
2020-2023
Erasmus MC
2020-2023
Amsterdam University Medical Centers
2019-2023
Amsterdam UMC Location University of Amsterdam
2009-2020
National Institute for Public Health and the Environment
2012-2020
TIB Molbiol (Germany)
2017
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of disease 2019 (COVID-19). Little known about interplay between preexisting immunity to endemic seasonal coronaviruses and development a SARS-CoV-2-specific IgG response. We investigated kinetics, breadth, magnitude, level cross-reactivity antibodies against SARS-CoV-2 heterologous epidemic at clonal in patients with mild or severe COVID-19 as well control patients. assessed antibody reactivity nucleocapsid spike...
(1) Background: Viral respiratory infections cause life-threatening diseases in millions of people worldwide every year. Human coronavirus and several picornaviruses are responsible for epidemic outbreaks, thus representing a heavy burden to their hosts. In the absence specific treatments human viral infections, natural products offer an alternative terms innovative drug therapies. (2) Methods: We analyzed antiviral properties leaves stem bark mulberry tree (Morus spp.). compared activity...
Human parechoviruses (HPeVs) are picornaviruses frequently infecting humans. While HPeV1 is associated with mild disease, HPeV3 the cause of neonatal sepsis and meningitis. To test whether in vitro replication kinetics could be related to pathogenicity, strains isolated from patients were cultured on cell lines gastrointestinal, respiratory neural origin, measured by real-time PCR. No relationship was found between clinical symptoms strains. In contrast, showed faster cells there a higher...
Since May 2022, over 21,000 mpox cases have been reported from 29 EU/EEA countries, predominantly among men who sex with (MSM). The Netherlands was the fourth most affected country in Europe, more than 1,200 and a crude notification rate of 70.7 per million population. first national case on 10 May, yet potential prior transmission remains unknown. Insight into prolonged undetected can help to understand current outbreak dynamics aid future public health interventions. We performed...
Human parechoviruses (HPeVs) and enteroviruses (EVs) belong to the family Picornaviridae. EVs are known cause a wide range of disease such as meningitis, encephalitis, sepsis. HPeV1 2 have been associated with mild gastrointestinal or respiratory symptoms in young children. HPeV3 is more severe neonatal infection. Little about epidemiology pathology HPeV4-6 children.We evaluated clinical children an HPeV 4, 5, 6 The patients positive stool samples were selected available plasma cerebrospinal...
Abstract The poorly studied picornavirus, human parechovirus 3 (HPeV3) causes neonatal sepsis with no therapies available. Our 4.3-Å resolution structure of HPeV3 on its own and at 15 Å in complex monoclonal antibody Fabs demonstrates the expected picornavirus capsid three distinct features. First, 25% RNA genome 60 sites is highly ordered as confirmed by asymmetric reconstruction, interacts conserved regions proteins VP1 VP3. Second, VP0 N terminus stabilizes inner surface, contrast to...
Abstract Severe acquired respiratory syndrome coronavirus-2 (SARS-CoV-2) is the cause of coronavirus disease (COVID-19). In severe COVID-19 cases, higher antibody titers against seasonal coronaviruses have been observed than in mild cases. To investigate cross-reactivity as potential explanation for disease, we determined kinetics, breadth, magnitude and level IgG SARS-CoV-2 CoV nucleocapsid spike from 17 cases at clonal level. Although patients mounted a mostly type-specific response,...
ABSTRACT Since it was first recognized in 2004 that human parechoviruses (HPeV) are a significant cause of central nervous system and neonatal sepsis, their clinical importance, primarily children, has started to emerge. Intravenous immunoglobulin treatment is the only available such life-threatening cases given moderate success. Direct inhibition parechovirus infection using monoclonal antibodies potential treatment. We have developed two neutralizing against HPeV1 HPeV2, namely, AM18 AM28,...
In May 2022, an outbreak of mpox (monkeypox) in men-who-have-sex-with-men (MSM) emerged and quickly affected over 100 countries. the early stages outbreak, overlap symptoms with sexually transmitted infections (STI) made triage for testing challenging. More information was needed on whom to screen main route transmission.We aimed identify characteristics cases further strengthen case definitions. We also compared Cycle threshold (Ct) values DNA positive samples as a proxy viral load by body...
Introduction. Mycoplasma genitalium is a sexually transmitted bacterium associated with non-gonococcal urethritis (NGU) in men. The rising macrolide and fluoroquinolone resistance M. has become public health concern, requiring close surveillance. Gap statement. MgpB/MG309 typing commonly used to study genotype distribution patterns of men who have sex (MSM); however, data for women (MSW) are limited. Aim. aim this was explore the epidemiology based on mgpB / MG309 molecular isolates from...
Human parechoviruses (HPeVs) are among the most frequently detected picornaviruses in humans. HPeVs usually associated with mild gastrointestinal and respiratory symptoms exception of HPeV3 which causes neonatal sepsis CNS infection. Previous studies showed various results culturing different HPeV genotypes, inducing only a low cytopathic effect (CPE).In vitro growth characteristics genotypes range 10 cell lines scored CPE measured supernatant by real time PCR. In optimal line for each...
Parechoviruses (PeVs) are highly prevalent viruses worldwide. Over the last decades, several studies have been published on PeV epidemiology in Europe, Asia and North America, while information other continents is lacking. The aim of this study was to describe circulation a cohort children Malawi, Africa. A total 749 stool samples obtained from Malawian aged 6 60 months were tested for presence by real-time PCR. We performed typing phylogenetic Basic Local Alignment Search Tool (BLAST)...
Abstract Understanding the coronavirus (CoV) antibody landscape in relation to disease and susceptibility is critical for modelling of steps next phase during current covid-19 pandemic. In March 2020, first month epidemic The Netherlands, we performed cross sectional studies at two time points amongst patients Erasmus Medical Centre Rotterdam, assess presence antibodies against seasonal human coronaviruses (OC43, 229E, NL63, HKU1), emerging zoonotic (SARS, MERS) SARS-CoV-2 nine different age...
The family Picornaviridae is a large and diverse group of positive-sense RNA viruses, including human enteroviruses (EVs) parechoviruses (HPeVs). immune response against EVs HPeVs thought to be mainly humoral, an insufficient neutralizing antibody (Ab) during infection risk factor can ultimately life threatening. accessibility different antigenic sites observed cross-reactivity make good target for development therapeutic monoclonal antibodies (MAbs). In this study, we generated two MAbs...
Human parechoviruses (HPeVs) are common pathogens in young children, and the Netherlands, HPeV1, HPeV3 HPeV4 most frequently detected genotypes. particular has been associated with severe disease infants below 3 months of age while other genotypes more often infect older children elicit mild symptoms. We investigated if maternal neutralizing antibodies (nAbs) against protect Dutch from related to HPeV infection.We conducted a prospective case-control study mother-infant pairs. Thirty-eight...
Enteroviruses (EVs) are among the most commonly detected viruses infecting humans worldwide. Although prevalence of EVs is widely studied, status EV in sub-Saharan Africa remains largely unknown. The objective our present study was therefore to increase knowledge on circulation Africa. We obtained 749 fecal samples from a cross-sectional conducted Malawian children aged 6 60 months. tested for presence using real time PCR, and typed positive based partial viral protein 1 (VP1) sequences. A...
Pleconaril is a capsid inhibitor used previously to treat enterovirus infections. A pleconaril-resistant echovirus 11 (E11) strain was identified before pleconaril treatment given in an immunocompromised patient. The patient also treated with intravenous Ig (IVIg) for long period but remained unresponsive. strains could not be neutralized vitro, confirming IVIg failure. To identify the basis of resistance, genetic and structural analyses were conducted. Analysis modelled viral indicated...
Background Meningococci produce a penta-acylated instead of hexa-acylated lipid A when their lpxL1 gene is inactivated. Meningococcal strains with such endotoxin variants have been found previously in adult meningitis patients, where they caused less blood coagulopathy because decreased TLR4 activation. Methods cohort 448 isolates from patients invasive meningococcal disease the Netherlands were screened for ability to induce IL-6 monocytic cell Mono Mac 6 cells. The was sequenced isolates,...
Pre-existing immunity played a significant role in protection during the latest influenza A virus H1N1 pandemic, especially older age groups. Structural similarities were found between A(H1N1)2009 and strains to which humans had already been exposed. Broadly cross-reactive antibodies capable of neutralizing have implicated this immune adults. We investigated serological profile group young children aged 9 years (n=55), from whom paired blood samples available, just prior pandemic wave (March...
SARS-CoV-2 prevention and control measures did not only impact circulation, but also the timing prevalence of other seasonal respiratory viruses. Especially in children, information on exposure infections to coronaviruses as well first year pandemic is largely lacking. Therefore, we set up a one-year serological survey large tertiary hospital Netherlands. We show that coronavirus seroprevalence significantly decreased 2021 children less than one year, most likely due COVID-19 measures. The...
Abstract Norovirus is a leading cause of epidemic acute gastroenteritis. More than 30 genotypes circulate in humans, some are common, and others only sporadically detected. Here, we investigated whether serology can be used to determine which infect children. We established multiplex protein microarray with structural non-structural norovirus antigens that allowed simultaneous antibody testing against human GI GII genotypes. Antibody responses sera obtained from 287 children aged < 1...
Abstract H7 avian influenza viruses represent a major public health concern, and worldwide outbreaks raise the risk of potential pandemic. Understanding memory B cell response to (H7) virus infection in humans could provide insights key human risks. We investigated an epizootic highly pathogenic A(H7N7) Netherlands, which 2003 led 89 persons one fatal case. Subtype-specificity antibodies were determined for confirmed H7N7 infected individuals (cases) (n = 19), contacts these cases 21)...