A5018445875- Viral Infections and Vectors
- SARS-CoV-2 and COVID-19 Research
- Mosquito-borne diseases and control
- Virology and Viral Diseases
- COVID-19 Clinical Research Studies
- Respiratory viral infections research
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Viral Infections and Outbreaks Research
- COVID-19 epidemiological studies
- SARS-CoV-2 detection and testing
- Vector-borne infectious diseases
- Long-Term Effects of COVID-19
- Vector-Borne Animal Diseases
- Influenza Virus Research Studies
- Fire effects on ecosystems
- Zoonotic diseases and public health
- Virus-based gene therapy research
- Animal Disease Management and Epidemiology
- HIV Research and Treatment
- COVID-19 Impact on Reproduction
- Complement system in diseases
- Malaria Research and Control
- Infectious Encephalopathies and Encephalitis
- Global Maternal and Child Health
Erasmus MC
2009-2025
Wageningen University & Research
2023-2024
National Institute for Public Health and the Environment
2002-2024
Erasmus University Rotterdam
2017-2024
Utrecht University
2022
University Medical Center Utrecht
2022
European Research Infrastructure on Highly Pathogenic Agents
2021-2022
European Clinical Research Infrastructure Network
2021-2022
The University of Texas Medical Branch at Galveston
2011-2019
St. Antonius Ziekenhuis
2016
Coronavirus in nonhuman primates We urgently need vaccines and drug treatments for coronavirus disease 2019 (COVID-19). Even under these extreme circumstances, we must have animal models rigorous testing of new strategies. Rockx et al. undertaken a comparative study three human coronaviruses cynomolgus macaques: severe acute respiratory syndrome–coronavirus (SARS-CoV) (2002), Middle East syndrome (MERS)–CoV (2012), SARS-CoV-2 (2019), which causes COVID-19 (see the Perspective by Lakdawala...
We investigated the natural history of human Calicivirus infection in community. Clinical information was obtained from 99 subjects infected with Norwalk-like viruses (NLV) and 40 Sapporo-like (SLV) a prospective, community-based cohort study. NLV common all age groups, whereas SLV mainly restricted to children aged <5 years. Symptoms lasted for median 5 6 days infections, respectively. Disease characterized by diarrhea during first (87% patients 95% infection) vomiting on day (74% 60% SLV)....
Abstract SARS-CoV-2, a coronavirus that emerged in late 2019, has spread rapidly worldwide, and information about the modes of transmission SARS-CoV-2 among humans is critical to apply appropriate infection control measures slow its spread. Here we show transmitted efficiently via direct contact air (via respiratory droplets and/or aerosols) between ferrets, 1 3 days 7 after exposure respectively. The pattern virus shedding indirect recipient ferrets similar inoculated infectious isolated...
ABSTRACT The viruses most commonly associated with food- and waterborne outbreaks of gastroenteritis are the noroviruses. lack a culture method for noroviruses warrants use cultivable model to gain more insight on their transmission routes inactivation methods. We studied reported enteric canine calicivirus no. 48 (CaCV) respiratory feline F9 (FeCV) correlated reduction in PCR units FeCV, CaCV, norovirus. Inactivation suspended was temperature time dependent range from 0 100°C. UV-B...
Halting transmission The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) glycoprotein binds to host cells and initiates membrane fusion cell infection. This stage in the virus life history is currently a target for drug inhibition. De Vries et al. designed highly stable lipoprotein inhibitors complementary conserved repeat C terminus of S that integrate into membranes inhibit conformational changes necessary fusion. authors tested performance lipoproteins as...
Abstract In a randomized clinical trial of 86 hospitalized COVID-19 patients comparing standard care to treatment with 300mL convalescent plasma containing high titers neutralizing SARS-CoV-2 antibodies, no overall benefit was observed. Using comprehensive translational approach, we unravel the virological and immunological responses following disentangle which may should be focus future studies. Convalescent is safe, does not improve survival, has effect on disease course, nor enhance viral...
The emergence and rapid spread of SARS-CoV-2 variants may affect vaccine efficacy substantially. Omicron variant termed BA.2, which differs substantially from BA.1 based on genetic sequence, is currently replacing in several countries, but its antigenic characteristics have not yet been assessed. Here, we used cartography to quantify visualize differences between early (614G, Alpha, Beta, Gamma, Zeta, Delta, Mu) using hamster antisera obtained after primary infection. We first verified that...
The severe acute respiratory syndrome coronavirus (SARS-CoV) caused a worldwide epidemic in late 2002/early 2003 and second outbreak the winter of 2003/2004 by an independent animal-to-human transmission. GD03 strain, which was isolated from index patient outbreak, reported to resist neutralization human monoclonal antibodies (hmAbs) 80R S3.1, can potently neutralize isolates first outbreak. Here we report that two hmAbs, m396 S230.15, neutralized representative SARS (Urbani, Tor2) palm...
Defining prospective pathways by which zoonoses evolve and emerge as human pathogens is critical for anticipating controlling both natural deliberate pandemics. However, predicting tenable of animal-to-human movement has been hindered challenges in identifying reservoir species, cultivating zoonotic organisms culture, isolating full-length genomes cloning genetic studies. The ability to design recover reconstituted from synthesized cDNAs the potential overcome these obstacles allowing...
Flaviviruses transmitted by arthropods represent a tremendous disease burden for humans, causing millions of infections annually. All vector-borne flaviviruses studied to date suppress host innate responses infection inhibiting alpha/beta interferon (IFN-alpha/beta)-mediated JAK-STAT signal transduction. The viral nonstructural protein NS5 some functions as the major IFN antagonist, associated with inhibition IFN-dependent STAT1 phosphorylation (pY-STAT1) or STAT2 degradation. West Nile...
ABSTRACT Several respiratory viruses, including influenza virus and severe acute syndrome coronavirus (SARS-CoV), produce more disease in the elderly, yet molecular mechanisms governing age-related susceptibility remain poorly studied. Advanced age was significantly associated with increased SARS-related deaths, primarily due to onset of early- late-stage distress (ARDS) pulmonary fibrosis. Infection aged, but not young, mice recombinant viruses bearing spike glycoproteins derived from early...
A neutralizing human monoclonal antibody can fully protect nonhuman primates from disease after a lethal Hendra virus challenge.
ABSTRACT Severe acute respiratory syndrome coronavirus (SARS-CoV) emerged in 2002, and detailed phylogenetic epidemiological analyses have suggested that it originated from animals. The spike (S) glycoprotein has been identified as a major component of protective immunity, 23 different amino acid changes were noted during the expanding epidemic. Using panel SARS-CoV recombinants bearing S glycoproteins isolates representing zoonotic human early, middle, late phases epidemic, we monoclonal...
In the 1990s, Hendra virus and Nipah (NiV), two closely related previously unrecognized paramyxoviruses that cause severe disease death in humans a variety of animals, were discovered Australia Malaysia, respectively. Outbreaks have occurred nearly every year since NiV was first discovered, with case fatality ranging from 10 to 100%. African green monkey (AGM), causes lethal respiratory and/or neurological essentially mirrors fatal human disease. Thus, AGM represents reliable model for...
ABSTRACT Nipah virus (NiV) and Hendra (HeV) are emerging zoonotic viruses the causative agents of severe respiratory disease encephalitis in humans. Little is known about mechanisms that govern development neurological disease. Using a hamster model lethal NiV HeV infection, we describe role route dose infection on clinical outcome determine tropism host responses following infection. Infection with high or resulted acute distress. initially replicated upper tract epithelium, whereas...
In the paramyxovirus cell entry process, receptor binding triggers conformational changes in fusion protein (F) leading to viral and cellular membrane fusion. Peptides derived from C-terminal heptad repeat (HRC) regions F have been shown inhibit by preventing formation of fusogenic six-helix bundle. We recently showed that addition a cholesterol group HRC peptides active against Nipah virus targets these where occurs, dramatically increasing their antiviral effect. this work, we report...
Abstract SARS-CoV-2, the causative agent of COVID-19 1 , features a receptor-binding domain (RBD) for binding to host cell ACE2 protein 1–6 . Neutralizing antibodies that block RBD-ACE2 interaction are candidates development targeted therapeutics 7–17 Llama-derived single-domain (nanobodies, ~15 kDa) offer advantages in bioavailability, amenability, and production storage owing their small sizes high stability. Here, we report rapid selection 99 synthetic nanobodies (sybodies) against RBD by...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of disease 2019 (COVID-19). Little known about interplay between preexisting immunity to endemic seasonal coronaviruses and development a SARS-CoV-2-specific IgG response. We investigated kinetics, breadth, magnitude, level cross-reactivity antibodies against SARS-CoV-2 heterologous epidemic at clonal in patients with mild or severe COVID-19 as well control patients. assessed antibody reactivity nucleocapsid spike...
Abstract The emergence and rapid spread of SARS-CoV-2 variants may impact vaccine efficacy significantly 1 . Omicron variant termed BA.2, which differs genetically substantially from BA.1, is currently replacing BA.1 in several countries, but its antigenic characteristics have not yet been assessed 2,3 Here, we used cartography to quantify visualize differences between using hamster sera obtained after primary infection. Whereas early are antigenically similar, clustering relatively close...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is associated with various neurological complications. Although the mechanism not fully understood, several studies have shown that neuroinflammation occurs in and post-acute phase. As these predominantly been performed isolates from 2020, it unknown if there are differences among SARS-CoV-2 variants their ability to cause neuroinflammation. Here, we compared neuroinvasiveness, neurotropism neurovirulence of ancestral...