A5047341769- Bacterial Infections and Vaccines
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Pneumonia and Respiratory Infections
- Influenza Virus Research Studies
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Biosensors and Analytical Detection
- Respiratory viral infections research
- Peripheral Neuropathies and Disorders
- Virology and Viral Diseases
- vaccines and immunoinformatics approaches
- CAR-T cell therapy research
- Data-Driven Disease Surveillance
- Immunodeficiency and Autoimmune Disorders
- Adenosine and Purinergic Signaling
- COVID-19 Clinical Research Studies
- COVID-19 epidemiological studies
- Poxvirus research and outbreaks
- Antifungal resistance and susceptibility
- Cystic Fibrosis Research Advances
- Cell Adhesion Molecules Research
- Animal Disease Management and Epidemiology
National Institute for Public Health and the Environment
2014-2024
Health Forecasting
2024
Erasmus MC
2021
Erasmus University Rotterdam
2021
National Centre for Infectious Diseases
2015
Leiden University
2008-2012
Leiden University Medical Center
2006-2012
Radboud University Nijmegen
2008
Radboud University Medical Center
2008
Abstract Rapid detection of infection is essential for stopping the spread severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The Roche SD Biosensor rapid antigen test SARS-CoV-2 was evaluated in a nonhospitalized symptomatic population. We rapid-tested sample onsite and compared results with those from reverse transcription PCR virus culture. analyzed date onset symptoms using data clinical questionnaire. Overall sensitivity 84.9% (95% CI 79.1–89.4) specificity 99.5% 98.7–99.8)....
To assess the performance of rapid antigen tests with unsupervised nasal and combined oropharyngeal self-sampling during omicron period.Prospective cross sectional diagnostic test accuracy study.Three public health service covid-19 sites in Netherlands, 21 December 2021 to 10 February 2022.6497 people symptoms aged ≥16 years presenting for testing.Participants had a swab sample taken reverse transcription polymerase chain reaction (RT-PCR, reference test) received one perform using either...
Abstract Dendritic cells (DCs) are specialized APCs with an important role in the initiation and regulation of immune responses. Immature DCs (iDCs) reportedly mediate tolerance absence maturation/inflammatory stimuli, presumably by induction regulatory T cells. In this study, we show for first time that repetitive iDC injections trigger expansion a novel population high immunomodulatory properties, able to protect mice from collagen-induced arthritis. These characterized expression CD49b...
Abstract Dendritic cells (DCs) are professional APCs which have the unique ability to present both foreign and self-Ags T steer outcome of immune responses. Because these characteristics, DCs attractive vehicles for delivery therapeutic vaccines. Fully matured relatively well-defined even used in clinical trials cancer. also potential influence autoimmunity by modulating underlying autoimmune response. To gain a better appreciation abilities mechanisms immunomodulatory cell responses, we...
The combination of immune-stimulating strategies has the potency to improve immunotherapy cancer. Vaccination against neoepitopes derived from patient tumor material can generate tumor-specific T cell immunity, which could reinforce efficacy checkpoint inhibitor therapies such as anti-PD-1 treatment. DNA vaccination is a versatile platform that allows inclusion multiple neoantigen-coding sequences in single formulation and therefore represents an ideal for neoantigen vaccination. We...
The demand for improved pertussis vaccines is urgent due to the resurgence of whooping cough. A deeper understanding mode action required achieve this improvement. vaccine-induced effects a candidate outer membrane vesicle vaccine (omvPV) and classical protective but reactogenic whole cell (wPV) were comprehensively compared in mice. comparison revealed essential qualitative quantitative differences with respect immunogenicity adverse these vaccines. Both stimulated mixed systemic...
Mucosal immunity is often required for protection against respiratory pathogens but the underlying cellular and molecular mechanisms of induction remain poorly understood. Here, systems vaccinology was used to identify immune signatures after pulmonary or subcutaneous immunization mice with pertussis outer membrane vesicles. Pulmonary led improved protection, exclusively induced mucosal immunoglobulin A (IgA) T helper type 17 (Th17) responses, in addition evoked elevated systemic G (IgG)...
Abstract Background Rapid detection of infectious individuals is essential in stopping the further spread SARS-CoV-2. Although rapid antigen test not as sensitive gold standard RT-PCR, time to result decreased by day(s), strengthening effectiveness contact tracing. Methods The Roche/SD Biosensor lateral flow was evaluated a mild symptomatic population at large drive through testing site. A second nasopharyngeal swab directly tested with on site and results were compared RT-PCR virus culture....
Worldwide resurgence of pertussis necessitates the need for improvement vaccines and vaccination strategies. Since natural infections induce a longer-lasting immunity than vaccinations, detailed knowledge immune responses following infection can provide important clues such improvement. The purpose was to elucidate kinetics protective response evolving after experimental Bordetella (B. pertussis) in mice. Data were collected from (i) individual analyses, i.e. microarray, flow cytometry,...
Abstract Background The diagnostic accuracy of unsupervised self-testing with rapid antigen tests (Ag-RDTs) is mostly unknown. We studied the a self-performed SARS-CoV-2 saliva and nasal Ag-RDT in general population. Methods This large cross-sectional study consecutively included unselected individuals aged $$\ge$$ <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:mo>≥</mml:mo> </mml:math> 16 years presenting for testing at three public health service test sites. Participants...
To assess the performances of three commonly used antigen rapid diagnostic tests as self-tests in asymptomatic individuals Omicron period.We performed a cross-sectional test accuracy study period public health service COVID-19 sites Netherlands, including 3600 aged ≥ 16 years presenting for SARS-CoV-2 testing any reason except confirmatory after positive self-test. Participants were sampled RT-PCR (reference test) and received one self-test (either Acon Flowflex [Flowflex], MP Biomedicals...
Abstract Background During the COVID-19 pandemic participatory digital syndromic surveillance systems proved itself, as it is scalable, flexible and function independent from health care system or seeking behaviour. A limitation of inability pathogen identification. We describe our experiences regarding integrating self-swabs with centralized testing into a in Netherlands ( Infectieradar ). Methods In 2022/2023 winter season was extended to include nose- throat swabs. Participants received...
Rapid antigen diagnostic tests (Ag-RDTs) are the most widely used point-of-care for detecting SARS-CoV-2 infection. Since accuracy may have altered by changes in epidemiology, indications testing, sampling and testing procedures, roll-out of COVID-19 vaccination, we evaluated performance three prevailing Ag-RDTs.
Effective immunity against Bordetella pertussis is currently under discussion following the stacking evidence of resurgence in vaccinated population. Natural more effective than vaccine-induced indicating that knowledge on infection-induced responses may contribute to improve vaccination strategies. We applied a systems biology approach comprising microarray, flow cytometry and multiplex immunoassays unravel molecular cellular signatures unprotected mice protected with immunity, around B....
Bordetella pertussis is a Gram-negative bacterium and the causative agent of whooping cough. Despite high vaccination coverage, outbreaks are being increasingly reported worldwide. Possible explanations include adaptation this pathogen, which may interfere with recognition by innate immune system. Here, we describe responses to different B. clinical isolates. By using HEK-Blue cells transfected pattern receptors, found that 3 out 19 isolates failed activate Toll-like receptor 4 (TLR4). These...
Abstract Current acellular pertussis (aP) vaccines promote a T helper 2 (Th2)-dominated response, while Th1/Th17 cells are protective. As our previous study showed, after adding non-toxic TLR4 ligand, LpxL1, to the aP vaccine in mice, Bordetella -specific Th2 response is decreased and responses increased as measured at cytokine protein level. However, how this shift Th by LpxL1 addition regulated gene expression level remains unclear. Transcriptomics analysis was performed on purified CD4 +...
In the last decade, several mumps outbreaks were reported in various countries despite high vaccination coverage. most cases, young adults affected who have acquired immunity against solely by and not previous wild-type virus infection. To investigate mumps-specific antibody levels, functionality dynamics during a epidemic, blood samples obtained longitudinally from 23 clinical with or without prior history of vaccination, 20 healthy persons no serological evidence recent Blood cases taken...
Pertussis, caused by infection with the gram negative B. pertussis bacterium, is a serious respiratory illness that can last for months. While rates are estimated between 1–10% in general population, notifications of symptomatic only comprise 0.01–0.1% indicating most individuals clear infections without developing (severe) clinical symptoms. In this study we investigated whether genetic risk factors involved development upon infection. Single-nucleotide polymorphisms (SNPs) candidate genes,...