Cécile A. C. M. van
A5007836143- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- vaccines and immunoinformatics approaches
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Virology and Viral Diseases
- Influenza Virus Research Studies
- Respiratory viral infections research
- COVID-19 Clinical Research Studies
- Immune Response and Inflammation
- Monoclonal and Polyclonal Antibodies Research
- Peripheral Neuropathies and Disorders
- Long-Term Effects of COVID-19
- HIV Research and Treatment
- Immunodeficiency and Autoimmune Disorders
- Pneumocystis jirovecii pneumonia detection and treatment
- Vaccine Coverage and Hesitancy
- Animal Virus Infections Studies
- Advanced Proteomics Techniques and Applications
- Cancer Immunotherapy and Biomarkers
- Hematopoietic Stem Cell Transplantation
- COVID-19 and healthcare impacts
- CAR-T cell therapy research
National Institute for Public Health and the Environment
2016-2025
Utrecht University
2021-2025
Health Forecasting
2023
Erasmus MC
2023
National Centre for Infectious Diseases
2015
Bioengineering Center
2013
National Center for Genetic Engineering and Biotechnology
2013
TuBerculosis Vaccine Initiative
2008
Institut de Recherche Vaccinale
2005
Leiden University
1986-1996
AbstractA multicentre, double blind, placebo controlled, randomised trial of nifedipine, metoprolol, and nifedipine metoprolol combined was conducted in a group 338 patients with unstable angina not pretreated beta blocker 177 blocker. The main outcome event recurrent ischaemia or myocardial infarction within 48 hours. Trial medication effects were expressed as ratios rates relative to placebo. In the rate associated 95% confidence intervals 1.15 (0.83, 1.64) for 0.76 (0.49, 1.16) 0.80...
Significance The surface presentation of peptides by human leukocyte antigen (HLA) class I molecules is essential for adaptive immune responses against pathogens and tumors. To date, the repertoire HLA remains largely unexplored, although their identity could lead to new targets vaccine development or cancer immunotherapy. Here, we demonstrate that recent developments in mass spectrometry (MS)-based sequencing technology can expand detectable peptide an unprecedented depth, revealing unique...
Mass spectrometry (MS)-based immunopeptidomics investigates the repertoire of peptides presented at cell surface by major histocompatibility complex (MHC) molecules. The broad clinical relevance MHC-associated peptides, e.g. in precision medicine, provides a strong rationale for large-scale generation immunopeptidomic datasets and recent developments MS-based peptide analysis technologies now support required data. Importantly, availability diverse has resulted an increasing need to...
Reestablishing self-tolerance in autoimmunity is thought to depend on self-reactive regulatory T cells (Tregs). Exploiting these antigen-specific regulators hampered by the obscure nature of disease-relevant autoantigens. We have uncovered potent disease-suppressive Tregs recognizing Heat Shock Protein (Hsp) 70 self-antigens, enabling selective activity inflamed tissues. Hsp70 a major contributor MHC class II ligandome. Here we show that conserved epitope (B29) present murine and upon...
Abstract During the COVID‐19 pandemic, daily life of many patients with dermatological immune‐mediated inflammatory diseases (DIMIDs), such as atopic dermatitis (AD), psoriasis, and vitiligo, was impacted by social restrictions caused (fear of) morbidity, mortality associated COVID‐19, vaccine hesitancy. This prospective observational, multicenter, multidisciplinary cohort study explored impact disease vaccination on DIMIDs, specifically AD, vitiligo. Data from DIMIDs were collected part...
We here show that anergic T cells are active mediators of cell suppression. In co-culture experiments, we found cells, derived from established rat clones and rendered via presentation the specific antigen (Ag), were inhibitors responses. Anergic inhibited not only responses with same Ag specificity as but also capable efficiently inhibiting polyclonal directed to other epitopes. This suppression required close cell-cell contact between antigen-presenting (APC), responder occurred when...
We report unexpected mass spectrometric observations of glycosylated human leukocyte antigen (HLA) class I-bound peptides. Complemented by molecular modeling, in vitro enzymatic assays, and oxonium ion patterns, we propose that the observed O-linked glycans carrying up to five monosaccharides are extended O-GlcNAc's rather than GalNAc-initiated O-glycans. A cytosolic O-GlcNAc modification is normally terminal does not extend produce a polysaccharide, but on an HLA peptide presents special...
Comprehensive analysis of the complex nature Human Leukocyte Antigen (HLA) class II ligandome is utmost importance to understand basis for CD4+ T cell mediated immunity and tolerance. Here, we implemented important improvements in repertoire HLA-DR-presented peptides, using hybrid mass spectrometry-based peptide fragmentation techniques on a sample isolated from matured human monocyte-derived dendritic cells (DC). The reported data set constitutes nearly 14 thousand unique high-confident...
Complex in vitro respiratory models, including air-liquid interface (ALI) transwell cultures and airway organoids, have emerged as promising tools for studying human virus infections. These models address several limitations of conventional two-dimensional cell line animal models. However, the lack standardized protocols application these infection studies limits possibilities comparing results across different research groups. Therefore, we applied a collaborative approach to harmonize...
After the COVID-19 pandemic, significant increases in measles cases were observed globally. Community-wide vaccination remains most effective strategy for preventing measles. However, it is crucial to understand whether prevalent genotypes, when circulating populations with suboptimal coverage, may undergo adaptive mutations that allow them escape vaccine-induced immunity. In this study, a bioinformatics-guided approach was used predict universal helper T-cell epitopes specific vaccine virus...
Human immunodeficiency virus type 1 (HIV-1)-infected H9 and blood mononuclear cells (MNCs) were studied by immunogold electron microscopy for the presence of HIV-1 gag p24 protein, env gp41 gp120 proteins, host cell molecules CD4, CD11a, CD25, CD54, CD63, HLA class I HLA-DR. Uninfected MNC membranes labelled II, and, at low density, CD11a CD54; lysosomal structures in cytoplasm CD63. The infected surface showed immunolabelling as did budding particle-like structures. Immunogold labelling...
The cytotoxic T cell (CTL) response is determined by the peptide repertoire presented HLA class I molecules of an individual. We performed in-depth analysis a broad panel common on four B lymphoblastoid cell-lines (BLCL). Peptide elution and mass spectrometry were utilised to investigate number abundance self-peptides. Altogether, 7897 unique self-peptides, derived 4344 proteins, eluted. After viral infection, self-peptides eluted significantly decreased compared uninfected cells, paralleled...
CD4+ T cells comprise multiple functionally distinct cell populations that play a key role in immunity. Despite blood monitoring of T-cell subsets is potential clinical utility, no standardized and validated approaches have been proposed so far. The aim this study was to design validate single 14-color antibody combination for sensitive reproducible flow cytometry human establish normal age-related reference values evaluate the presence potentially altered profiles three disease...
Despite high vaccination coverage, Bordetella pertussis the causative agent of whooping cough is still a health concern worldwide. A resurgence cases has been reported, particularly in countries using acellular vaccines with waning immunity and pathogen adaptation thought to be responsible. better understanding protective immune responses needed for development improved vaccines. In our study, B. strain B1917 variants presenting single gene deletion were generated analyze role vaccine...
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has led to considerable morbidity/mortality worldwide, but most infections, especially among children, have a mild course. However, it remains largely unknown whether infected children develop cellular immune memory.To determine memory T cell response is being developed, we performed longitudinal assessment of the SARS-CoV-2-specific by IFN-γ ELISPOT and activation marker analyses peripheral blood samples from unvaccinated adults...