A5089087944- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Peptidase Inhibition and Analysis
- Ubiquitin and proteasome pathways
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Advanced Proteomics Techniques and Applications
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Glycosylation and Glycoproteins Research
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Cancer Immunotherapy and Biomarkers
- Spondyloarthritis Studies and Treatments
- Protein Kinase Regulation and GTPase Signaling
- Mass Spectrometry Techniques and Applications
- Reproductive biology and impacts on aquatic species
- Cancer Research and Treatments
- RNA and protein synthesis mechanisms
- Melanoma and MAPK Pathways
- Cell Image Analysis Techniques
- Endoplasmic Reticulum Stress and Disease
- Potato Plant Research
- Rheumatoid Arthritis Research and Therapies
- Fungal and yeast genetics research
Technion – Israel Institute of Technology
2015-2024
University Hospital Heidelberg
2019
Leiden University Medical Center
2019
Immatics Biotechnologies (Germany)
2019
BioNTech (United States)
2019
Johannes Gutenberg University Mainz
2019
Heidelberg University
2019
University of Namur
2016
Flagstaff Medical Center
2008
Kaplan (United States)
1996
Abstract HUPO initiated the Plasma Proteome Project (PPP) in 2002. Its pilot phase has (1) evaluated advantages and limitations of many depletion, fractionation, MS technology platforms; (2) compared PPP reference specimens human serum EDTA, heparin, citrate‐anti‐coagulated plasma; (3) created a publicly‐available knowledge base (www.bioinformatics.med.umich.edu/hupo/ppp; www.ebi.ac.uk/pride). Thirty‐five participating laboratories 13 countries submitted datasets. Working groups addressed...
Human AP-2 is a sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to stimulate transcription of selected genes. Here, we report the isolation characterization human cDNA clone containing entire protein-coding region AP-2. The deduced primary amino acid sequence does not contain domain resembling any previously identified DNA binding motif. However, an interesting feature clustered arrangement proline glutamine residues have been found...
Enhancer binding protein AP-4 is a transcription factor that activates both viral and cellular genes by to the symmetrical DNA sequence, CAGCTG. Here, we report molecular cloning characterization of human cDNAs. The deduced amino acid sequence reveals helix-loop-helix (HLH) protein. Like other members this family, HLH motif adjacent basic domain are necessary sufficient confer site-specific binding. However, unlike proteins, also contains two additional dimerization motifs consisting leucine...
RNA polymerase I and II transcription factors SL1 TFIID, respectively, are composed of the TATA-binding protein (TBP) a set TBP-associated (TAFs) responsible for promoter recognition. How universal factor TBP becomes committed to TFIID or complex has not been known. Complementary DNAs encoding each three TAF s that integral components have now isolated. Analysis subunit interactions indicated can bind individually specifically TBP. In addition, these interact with other form stable TBP-TAF...
Dimerization among transcription factors has become a recurrent theme in the regulation of eukaryotic gene expression. Hepatocyte nuclear factor-1α (HNF-1α) is homeodomain-containing protein that functions as dimer. A dimerization cofactor HNF-1α (DCoH) was identified displayed restricted tissue distribution and did not bind to DNA, but, rather, selectively stabilized HNF-1 alpha dimers. The formation stable tetrameric DCoH-HNF-1 complex, which required domain alpha, change DNA binding...
Phosphatidylinositol (PtdIns) 4-kinase catalyzes the first step in biosynthesis of PtdIns-4,5- bis phosphate (PtdIns[4,5]P 2 ). Hydrolysis PtdIns[4,5]P response to extracellular stimuli is thought initiate intracellular signaling cascades that modulate cell proliferation and differentiation. The PIK1 gene encoding a PtdIns from yeast Saccharomyces cerevisiae was isolated by polymerase chain reaction (PCR) with oligonucleotides based on sequence peptides derived purified enzyme. product bears...
A human apurinic/apyrimidinic endonuclease activity, called AP I, is missing from or altered specifically in cells cultured Xeroderma pigmentosum group-D individuals (XP-D cells) (Kuhnlein, U., Lee, B., Penhoet, E. E., and Linn, S.(1978) Nucleic Acids Res. 5, 951-960). We have now observed that another nuclease UV III, similarly not detected XP-D inseparable the I activity. This activity preferentially cleaves phosphodiester backbone of heavily ultraviolet-irradiated DNA at unknown lesions...
Tandem mass spectrometry (MS/MS), coupled with liquid chromatography (LC), is a powerful tool for the analysis and comparison of complex protein peptide mixtures. However, extremely large amounts data that result from process are very difficult to analyze. We show how clustering similar spectra multiple LC-MS/MS runs can help in management improve The major effect spectrum reduction huge manageable size. As result, time shorter more be stored further analysis. Furthermore, quality...
The ubiquitin-proteasome pathway plays a crucial role in many cellular processes by degrading substrates tagged polyubiquitin chains, linked mostly through lysine 48 of ubiquitin. Although polymerization ubiquitin via its six other residues exists vivo as part various physiological pathways, the molecular mechanisms that determine type chains remained largely unknown. We undertook systematic, vitro, approach to evaluate E2 enzymes determining topology polyubiquitin. Because this study was...
Significance A substrate-conjugated polyubiquitin chain is accepted as the “canonical” proteasomal degradation signal. Using a cellular (human and yeast) proteomic screen in exclusive presence of nonpolymerizable ubiquitin, we show that large group proteins degraded by proteasome following monoubiquitination. The also unraveled polyubiquitin-dependent substrates, they are stabilized this ubiquitin mutant. Notably, monoubiquitination- polyubiquitination-dependent substrates display distinct...
// Shelly Kalaora 1 , Eilon Barnea 2,* Efrat Merhavi-Shoham 3,* Nouar Qutob Jamie K. Teer 4 Nilly Shimony 3 Jacob Schachter Steven A. Rosenberg 5 Michal J. Besser 3,6,** Arie Admon 2,** and Yardena Samuels Department of Molecular Cell Biology, Weizmann Institute Science, Rehovot, Israel 2 Technion, Haifa, The Ella Lemelbaum for Melanoma, Chaim Sheba Medical Center, Tel Hashomer, Biostatistics Bioinformatics, H. Lee Moffitt Cancer Center Research Institute, Tampa, FL, USA National NIH, MD, 6...